DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20170473

Anti-inflammatory activity of hydroalcoholic extract of mimosa pudica whole plant in rats

Parvathy Velayudhan Nair, Bindu Latha R. Nair

Abstract


Background: Mimosa pudica is a traditionally used folk medicine to treat various disorders like infections, anxiety, depression, bleeding disorders, convulsions, rheumatoid arthritis, muscular pain, asthma, snake bite etc. We evaluated the anti-inflammatory activity of hydroalcoholic extract of Mimosa pudica whole plant (HAEMPWP) in rats.

Methods: HAEMPWP was prepared using Soxhlets apparatus. Acute toxicity tests were done with HAEMPWP given orally to albino rats in increasing doses up to 3200 mg/ kg body weight. The anti-inflammatory action was evaluated by Carrageenan induced paw edema method. Thirty albino rats were grouped into five groups and each contained six rats. Group I (control group) received distilled water orally. Group II (standard) received Aspirin orally dissolved in distilled water. Groups III, IV and V received HAEMPWP in doses of 200 mg/kg, 400 mg/kg and 800mg/kg orally dissolved in distilled water. Data analysis was done by one way ANOVA and unpaired t test using SPSS 16 for windows.

Results: HAEMPWP showed a significant anti-inflammatory activity as compared to control. There was no statistically significant dose dependent increase in the anti-inflammatory activity.

Conclusions: HAEMPWP possesses significant anti-inflammatory activity and could be an effective treatment option for various inflammatory conditions.

Keywords


Albino rats, Anti-inflammatory, Carrageenan, Mimosa pudica, Soxhlet apparatus

Full Text:

PDF

References


Katzung BG. Basic and clinical Pharmacology. 10th ed. New York: Mc Graw Hill; 2007:314.

Mitchell RN, Cotran RS. In: Kumar V, Cotran RS, Robbins SL. (Eds). Basic Pathology. 6th ed. Bangalore: Prism Books Pvt Ltd; 1997:25-46.

Rang HP, Dale MM, Ritter JM, Flower RJ. Pharmacolgy. 6th ed. Elseiver: Churchill Livingstone; 2007:218-20.

Satoskar RS, Rege NN, Bhandarkar SD. Pharmacology and Pharmacotherapeutics. 24th ed. Mumbai: Popular Prakasan; 2015:168.

Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in hind paw of the rat as an aasay for anti-inflammatory drugs. Proc Soc Exp Biol Med. 1962;3:544-7.

Nadkarni KM. Indian Materia Medica. 3rd ed. Vol 1. Bombay: Popular Prakashan pvt Ltd; 1976:799-800.

Howard RA. Flora of the Lesser Antilles, Leewards and Windward Islands in Dicotyledonea, Part 1, Jamaica Plain MA: Arnold Arboretum, Harvard University; 1988;4:673.

Satyavati G, Gupta AK, Tandon N. Medicinal plants of India.3rd ed.Vol 2. Bombay: Popular Prakashan pvt Ltd; 1987:254-6.

Kirtikar KR, Basu BD. Indian medicinal plants. 2nd ed. Vol 2. Dehradun: Bishen Singh Mahendrapal Singh; 1935:915-17.

Warrier PK, Nambiar VPK, Ramankutty C. Indian Medicinal Plants, a compendium of 500 species. 1st ed. Vol5. Madras: Orient Longman Ltd; 1997:263.

Tamilarasi T, Ananthi T. Phytochemical analysis and antimicrobial activity of Mimosa pudica Linn. Res J Chem Sci. 2012;2:72-4.

Hafsa A, Sakshi S, Anurag M, Rajiv G. Mimosa pudica L. (Laajvanthi): An overview. Pharmacogn Rev. 2012;6:115-24.

Sengupta KNN. The Ayurvedic system of Medicine. 2nd ed. Vol1. New Delhi: Sri Satguru Publications; 1987:374-5.

Tirtha SSS. The Ayurvedic Encyclopedia: Natural secrets for healing, prevention and longevity. 1st ed. New Delhi: Sri Satguru Publications; 1976:214.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi: CSIR; 1988:167.

Bahekar PC, Shaikh HY, Nigade PB, Ghaisas MM. Antihistaminic activity of aqueous extract of Mimosa pudica Linn. J Pharm Research. 2007;63:134-8.

Nair PV, Nair B. Muscle relaxant activity of hydroalcoholic extract of Mimosa pudica whole plant in mice. Natl J Physiol Pharm Pharmacol. 2017;7 (online first).

Vejayan J, Ibrahim H, Othman I. The potential of Mimosa pudica against snake envenomation. J Trop Forest Sci. 2007;19:189-97.

Rosenthaler L. The chemical investigations of plants. London: G Bell and sons Ltd; 1930:67.

Ghosh MN. Fundamentals of experimental Pharmacology. 2nd ed. Calcutta: Hilton and Company. 2005:190-4.

Singh H, Ghosh MN. Modified plethysmometer for measuring foot volume of unanaesthetised rats. J Pharm Pharmacol. 1968;20:316-7.

Vikram PK, Malavi R, Jain DK. Evaluation of analgesic and anti-inflammatory potential of Mimosa pudica Linn. Int J Curr Pharm Res. 2012;4:47-50.

Vogel HG, Vogel WH. Drug Discovery and Evaluation: Pharmacological assays. 2nded. New York: Springer - Verlag Berlin Heidelberg. 2002:759-61.

Garaa MD, Fernandez MA, Alvaraz A, Saenz MT. Antinociceptive and anti-inflammatory effect of the aqueous extract from leaves of Pimenta racemosa var ozua (Myrtaceae). J Ethn Pharm. 2004;91:69-73.

Brooks PM, Day RO. Nonsteroidal antiinflammatory drugs- differences and similarities. N Engl J Med. 1991;324:1716-25.

Mistry S, Patidar R, Vyas V, Jena J, Dutt KR. Anti-inflammatory activity of Mimosa pudica Linn (Mimosaceae) leaves: An ethnopharmacological study. J Pharm Sci Res. 2012;4:1789-91.

Fowzy AA, Vishwanath BS, Frason RC. Inhibition of human non-pancreatic phospholipase A2 by retinoids and flavonoids. Mechanism of Action. Agents Action. 1988;25:394.

Ferrandiz ML, Alcaraz MJ. Anti-inflammatory activity and inhibition of arachidonic acid metabolism by flavonoids. Agents Action. 1991;32:283-8.

Aitchdrfoun M, Mounnier C, Heymons F, Bon C, Binistic C, Godfroid JJ. 4-Alkoxybenzamidines as new potent phospholipase A2 inhibitors. Biochem Pharmacol. 1996;51:737.

Goli V, Bhaskar KV, Macharla SP, Bhaskar J, Devi PS, Ramchander T. Effects of anti-inflammatory activity of Mimosa pudica. Asian J Pharm Res. 2011;1:69-71.