Evaluation of sesamol for anxiolytic activity in 72 hours sleep deprivation model in C57BL/6 mice

Authors

  • Richa Singh Department of Pharmacology, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, India
  • Shilpa N. Koare Department of Pharmacology, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, India
  • Ajay K. Shukla Department of Pharmacology, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20231889

Keywords:

Sesamol, C57BL/6 mice, 72 hours sleep deprivation, Anxiolytic activity

Abstract

Background: In our study, we explored anxiolytic activity of sesamol in C57BL/6 mice. Anxiety was induced in mice after 72 hours of sleep deprivation. Anxiety was assessed by three behavioural tests as Open field test, hole board test and light dark chamber test.

Methods: In our study, sesamol was evaluated in C57BL/6 mice for anxiolytic activity. Anxiety was induced by flower pot technique, in which sleep deprivation was done in mice for 72 hours. After 72 hours of sleep deprivation, the animals were subjected to three behavioural tests, as open field test, Hole board test and Light dark chamber test. In the Open field test, parameter assessed was time spent in central square, that was considered as a measure of anxiolytic activity. In hole board test, increase in number of head dips was indicative of anxiolytic activity. Anxiety assessment was also done by light dark chamber test, where more time spent in light chamber is considered as indicative of anxiolytic activity.

Results: In open field test, sesamol 20 mg/kg group, the time spent in central square was statistically significant (p<0.05) in post hoc comparison, suggesting that sesamol exhibited anxiolytic effect in animals. In hole board test, sesamol 20 mg/kg group showed significant increase (p<0.05) in number of head dips indicating its anxiolytic activity in the said model. In light dark chamber test, there is significant increase in anxiolytic activity in diazepam group as compared to control group, while not significant with sesamol in either dose in light dark chamber test.

Conclusions: So, to conclude, sesamol exhibited statistically significant anxiolytic activity in the dose of 20 mg/kg in open field test and hole board test.

References

Bandelow B. Current and Novel Psychopharmacological Drugs for Anxiety Disorders. In: Kim, YK. (eds) Anxiety Disorders. Advances in Experimental Medicine and Biology Springer, Singapore. 2020;1191.

Nash JR, Nutt DJ. Pharmacotherapy of anxiety. Handb Exp Pharmacol. 2005;(169):469-01.

Logan A, Marsden J, Freeman J, Kent B. Effectiveness of non-pharmacological interventions in treating orthostatic hypotension in the elderly and people with a neurological condition: a systematic review protocol. JBI Evid Synth. 2017;15:948-60.

Sharifi-Rad M, Lankatillake C, Dias DA, Docea AO, Mahomoodally MF, Lobine D et al. Impact of Natural Compounds on Neurodegenerative Disorders: From Preclinical to Pharmacotherapeutics. J Clin Med. 2020;9:1061.

Moradi SZ, Momtaz S, Bayrami Z, Farzaei MH, Abdollahi M. Nanoformulations of Herbal Extracts in Treatment of Neurodegenerative Disorders. Front Bioeng Biotechnol. 2020;8:238.

Katz L, Baltz RH. Natural product discovery: past, present, and future. J Ind Microbiol Biotechnol. 2016;43:155-76.

Majdaeen M, Banaei A, Abedi-Firouzjah R, Ebrahimnejad Gorji K, Ataei G, Momeni F et al. Investigating the radioprotective effect of sesamol oral consumption against gamma irradiation in mice by micronucleus and alkaline comet assays. Appl Radiat Isot. 2020;159:109091.

Siran R, Ahmad AH, Abdul Aziz CB, Ismail Z. REM sleep deprivation induces changes of down regulatory antagonist modulator (DREAM) expression in the ventrobasal thalamic nuclei of Sprague Dawley rats. J Physiol Biochem. 2014; 70:877-89.

Garg R, Kumar A. Possible role of citalopram and desipramine against sleep deprivation-induced anxiety like-behaviour alterations and oxidative damage in mice. Indian J Exp Biol. 2008;46:770-6.

Seibenhener ML, Wooten MC. Use of the Open Field Maze to measure locomotor and anxiety-like behavior in mice. J Vis Exp. 2015;(96):e52434.

Kamei J, Matsunawa Y, Miyata S, Tanaka S, Saitoh A. Effects of nociceptin on the exploratory behavior of mice in the hole-board test. Eur J Pharmacol. 2004;489:77-87.

Campos AC, Fogaça MV, Aguiar DC, Guimarães FS. Animal models of anxiety disorder stress. Braz J Psychiatry. 2013;35:S101-11.

Kumar A, Kaur G, Kalonia H, Rinwa P. Evaluation of sesamol and buspirone in stress induced anxiety in mice. Indian J Pharmacol. 2013;45:49-53.

Papp M, Litwa E, Gruca P, Mocaër E. Anxiolytic-like activity of agomelatine and melatonin in three animal models of anxiety. Behav Pharmacol. 2006;17:9-18.

Bourin M. Animal models for screening anxiolytic-like drugs: a perspective. Dialogues Clin Neurosci. 2015;17:295-303.

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Published

2023-06-27

How to Cite

Singh, R., Koare, S. N., & Shukla, A. K. (2023). Evaluation of sesamol for anxiolytic activity in 72 hours sleep deprivation model in C57BL/6 mice. International Journal of Basic & Clinical Pharmacology, 12(4), 545–550. https://doi.org/10.18203/2319-2003.ijbcp20231889

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Original Research Articles