DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20170457

Efficcacy of Lantana camara Linn. leaf extracts ointment on dermal wound healing were infected with Staphylococcus epidermidis

Mauritius Lambertus Edy Parwanto

Abstract


Background: Dermal infections by Staphylococcus epidermidis can cause problems for patients. Antibiotic resistance have been reported to treat the bacteria, therefore need to develop new drugs, among others sourced from herbs. For this purpose, study is needed to find an efficacy of Lantana camara Linn. leaf extract ointment on dermal wound healing were infected with S. epidermidis.

Methods: Wistar rats divided into 4 groups randomly. Group I=dermal wounded, infected with S. epidermidis, untreated. Group II=dermal wounded, infected with S. epidermidis, treated with L. camara Linn. leaf extract ointment 5%. Group III=dermal wounded, infected with S. epidermidis, treated with L. camara Linn. leaf extract ointment 10%. Group IV=dermal wounded, infected with S. epidermidis, treated with sodium fusidate 2%. Bacterial colonies number observed on 6 days, while the quality of wound healing, measurement of DNA and protein levels on 6, 14 and 18 days. Mean ± SD between groups were analyzed by ANOVA.

Results: Wound healing in group II qualitatively better than group I, III and IV. Bacterial colonies number in group II was lower than groups I, III and IV (p<0.05), whereas DNA and protein levels was higher in group II compare to groups I, III and IV (p<0.05). On 6, 14 and 18 days observations there are trend similarly ie DNA levels decreased, conversely protein levels increased.

Conclusions: L. camara Linn. leaf extract ointment 5% more effective than 10% on dermal wound healing.


Keywords


DNA, Lantana camara Linn., Protein, Staphylococcus epidermidis, Wound healing

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References


Martin P, Nunan R. Cellular and molecular mechanisms of repair in acute and chronic wound healing. Br J Dermatol. 2015;173(2):370-8.

Clark RA. Fibrin and wound healing. Annals of the New York Acad of Sci. 2001;936:355-67.

Bhat RS, Shankrappa J, Shivkumar HG. Formulation and evaluation of polyherbal wound treatments. Asia J Pharma Sci. 2007;2(1):11-7.

Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008;453:314-21.

Wall SJ, Sampson MJ, Levell N, Murphy G. Elevated Matrix metallo proteinase 2 and 3- production from human diabetic dermal fibroblast. Brit J Dermatol. 2003;149(1):13-6.

von Eiff C, Peters G, Heilmann C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infect Dis. 2002;2:677-85.

Gandelman G, Frishman WH, Wiese C, Green-Gastwirth V, Hong S, Aronow WS, et al. Intravascular device infections: Epidemiology, diagnosis, and management. Cardiol Rev. 2007;15(1):13-23.

Joo SS, Jang SK, Kim SG. Anti-acne activity of Selagineela involvens extract and its non antibiotic anti-microbial potential on Propionibacterium acnes. Phytother Res. 2008;22:335-9.

McCann MT, Gilmore BF, Gorman SP. Staphylococcus epidermidis device-related infections: pathogenesis and clinical management. J of Pharm and Pharmacol. 2008;60(12):1551-71.

Arrecubieta C, Toba FA, von Bayern M, Akashi H, Deng MC, Naka Y, et al. A Staphylococcus epidermidis Surface Protein, Contributes to the Initiation of Ventricular Assist Device Driveline-Related Infections. PLoS Pathog. 2009;5(5):1-13.

Suetens C, Morales I, Savey A. European surveillance of ICU-acquired infections (HELICS-ICU): methods and main results. J of Hospital Infect. 2007;65(2):171-3.

Schoenfelder SMK, Lange C, Eckart M, Hennig S, Kozytska S, Ziebuhr W. Success through diversity- how Staphylococcus epidermidis establishes as a nosocomial pathogen. Int J of Med Microbiol. 2010;300(6):380-6.

Kalita S, Kumar G, Karthik L, Rao KVB. A Review on Medicinal Properties of Lantana camara Linn. Research J Pharm and Tech. 2012;5(6):711-5.

Bhakta D, Ganjewala D. Effect of leaf positions on total phenolics, flavonoids and proantho-cyanidins content and antioxidant activities in Lantana camara (L). J of Scient Res. 2009;1(2):363-9.

Venkatachalam T, Kumar VK, Selvi PK, Maske AO, Kumar NS. Physicochemical and preliminary phytochemical studies on the Lantana Camara (L.) fruits. Int J of Pharm and Pharmaceut Scienc. 2011;3(1):52-4.

Kensa VM. Studies on phytochemical screening and antibacterial activities of Lantana camara Linn. Plant Sciences Feed. 2011;1(5):74-9.

Ganjewala D, Sam S, Khan KH. Biochemical compositions and antibacterial activities of Lantana camara plants with yellow, lavender, red and white flowers. EurAsian J of BioSci. 2009;3:69-77.

Barreto FS, Sousa EO, Campos AR, Costa JGM, Rodrigues FFG. Antibacterial activity of Lantana camara Linn and Lantana montevidensis brig extracts from Cariri-Ceará, Brazil. J of Young Pharm. 2010;2(1):42-4.

Badakhshan MP, Sasidharan S, Rameshwar NJ, Ramanathan S. A comparative study: antimicrobial activity of methanol extracts of Lantana camara various parts. Pharmacog Res. 2009;1(6):348-51.

Srivastava D, Singh P. Antifungal potential of two common weeds against plant pathogenic fungi- Alternaria sps. Asian J of Experiment Biol Scienc. 2011;2(3):525-8.

Thamotharan G, Sekar G, Ganesh T, Sen S, Chakraborty R, Kumar S. Antiulcerogenic effects of Lantana camara Linn. leaves On in vivo test models in rats. Asian J of Pharm and Clin Res. 2010;3(3):57-60.

Nayak BS, Raju SS, Eversley M, Ramsubha A. Evaluation of wound healing activity of Lantana camara L. - a preclinical study. Phytother Res. 2009;23(2):241-5.

Hosea JE. Antibacterial opaque soap formula of Lantana camara L. leaf extract on Staphylococcus epidermidis (in Indonesia language). Progdi Farmasi-FMIPA, Universitas Sam Ratulangi, Manado, Sulawesi Utara, Indonesia; 2012.

Parwanto E, Senjaya H, Jaya Edy H. Antibacterial ointment formulation of Lantana camara L. leaf ethanol extract (in Indonesia language). Pharmacon. 2013;2(03):104-8.

Hirsch T, Spielmann M, Zuhaili B, Koehler T, Fossum M, Steinau HU, et al. Enhanced susceptibility to infections in a diabetic wound healing model. BMC Surg. 2008;8(5):1-8.

O'Meara S, Nelson EA, Golder S, Dalton JE, Craig D, Iglesias C. Systematic review of methods to diagnose infection in foot ulcers in diabetes. Diabetic Med. 2006;23:341-7.

Somboonwong J, Kankaisre M, Tantisira B,Tantisira MH. Wound healing activities of different extracts of Centella asiatica in incision and burn wound models: an experimental animal study. BMC Compl Altern Med. 2012;12(103):1-7.

Sasidharan S, Logeswaran S, Latha LY. Wound healing activity of Elaeis guineensis leaf extract ointment. Int J Mol Sci. 2012;13(1):336-47.

Chaushu L, Weinreb M, Beitlitum I, Moses O, Nemcovsky CE. Evaluation of a topical herbal patch for soft tissue wound healing: an animal study. J of Clin Periodontol. 2015;42:288-93.

Perini JA, Angeli-Gamba T, Alessandra-Perini J, Ferreira LC, Nasciutti LE, Machado DE. Topical application of Acheflan on rat skin injury accelerates wound healing: a histopathological, immunohistochemical and biochemical study. BMC Compl and Altern Med. 2015;15(203):1-8.

Büttner H, Mack D, Rohde H. Structural basis of Staphylococcus epidermidis biofilm formation: mechanisms and molecular interactions. Frontiers in Cell and Infect Microbiol. 2015;5(14):1-15.

Otto M. Staphylococcal biofilms. Curr Top Microbiol Immunol. 2008;322:207-28.

Zhao G, Usui ML, Lippman SI, James GA, Stewart PS, Fleckman P, et al. Biofilms and Inflammation in Chronic Wounds. Adv in wound care, 2013;2(7):389-99.

Siddiqui AR, Bernstein JM. Chronic wound infection: facts and controversies. Clin Dermatol. 2010;28:519-26.

Parsek MR, Singh PK. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 2003;57:677-701.

Schierle CF, De la Garza M, Mustoe TA, Galiano RD. Staphylococcal biofilms impair wound healing by delaying reepithelialization in a murine cutaneous wound model. Wound Repair Regen. 2009;17(3):354-9.

Grice EA, Segre JA. Interaction of the microbiome with the innate immune response in chronic wounds. Adv Exp Med Biol. 2012;946:55-68.

Lin MH, Chang FR, Hua MY, Wu YC, Liu ST. Inhibitory effects of 1, 2, 3, 4, 6-penta-O-galloyl- β-D-glucopyranose on biofilm formation by Staphylococcus aureus. Antimicrob Agents and Chemother. 2011;55(3):1021-7.

Escaich S. Antivirulence as a new antibacterial approach for chemotherapy. Current Op in Chem Biol. 2008;12(4):400-8.

Chusri S, Sompetch K, Mukdee S, Jansrisewangwong S, Srichai T, Maneenoon K, et al. Inhibition of Staphylococcus epidermidis Biofilm Formation by Traditional Thai Herbal Recipes Used for Wound Treatment. Evidence-Based Compl and Altern Med. 2012:1-8.

Fujii T, Wakaizumi M, Ikami T, Saito M. Amla (Emblica officinalis Gaertn.) extract promotes procollagen production and inhibits matrix metalloproteinase-1 in human skin fibroblast. J Ethnopharmacol. 2008;119(1):53-7.

Sumitra M, Manikandana P, Suguna L. Efficacy of Butea monosperma on dermal wound healing in rats. www.aseanbiodiversity.info/.../51003935.pdf. 2004; asseced on January 12; 2013;11.42.

Cooper L, Johnson C, Burslem F, Martin P. Wound healing and inflammation genes revealed by array analysis of ‘macrophageless’ PU.1 null mice. Genome Biol. 2004;6(1)R5:1-17.

Lewis CJ, Mardaryev AN, Sharov AA, Fessing MY, Botchkarev VA. The Epigenetic Regulation of Wound Healing. Adv in wound care. 2014;3(7):468-75.

Rosique RG, Rosique MJ, Jayme AFJ. Curbing Inflammation in Skin Wound Healing: A Review. Int J Inflam. 2015;316235:1-9.

Buemi M, Galeano M, Sturiale A. Recombinant human erythropoietin stimulates angiogenesis and healing of ischemic skin wounds, Shock. 2004;22(2):169-73.

Abdulla MA, Hassandarvish P, Ali HM, Noor SM, Mahmoud FH, Bashah NSA, et al. Acceleration of Wound Healing Potential by Lantana camara Leaf Extract in Experimental Rats. Res J of Med Scienc. 2009;3(2):75-9.