A clinical study of patients with central venous catheter associated bloodstream infections in a tertiary care hospital


  • R. Abisha Rezia Department of Pharmacology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India
  • R. Vijendra Department of Pharmacology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India
  • Anjana Gopi Department of Microbiology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India




Central venous catheter, Bacteriological profile, Antimicrobial drug resistance


Background: Central venous access puts the patients at risk of iatrogenic complications and is associated with bloodstream infections. Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus and Methicillin Resistant Staphylococcus aureus (MRSA) are responsible for at least two-thirds of the infections followed by Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Enterococcus spp and Acinetobacter spp. Due to the scarcity of Central Venous Catheter associated Blood Stream Infections (CVC-BSI) data, this study was taken up in our tertiary care hospital.

Aims: This study is aimed to study the profile of organisms causing CVC-BSI, assess their antimicrobial susceptibility, the clinical course and outcome.

Methods: All subjects whose central venous catheter samples (n=84) were sent for culture and sensitivity during the study period were included in this prospective observational study. The study was done in the Department of Microbiology from July 2019 to December 2019. The catheter tips were streaked onto blood agar plate using Roll plate technique. After biochemical identification of the organisms, antimicrobial susceptibility testing was performed by modified Kirby-Bauer disc diffusion method as per the Clinical Laboratory Standard Institute (CLSI) guidelines.

Results: Growth of pathogens was seen in 64.3% (n=54). The common organisms were Coagulase Negative Staphylococcus aureus (CONS) in 27.78% (n=15), Enterococcus spp, Klebsiella pneumoniae in 14.8% each (n=8) and Acinetobacter spp in 11.1% (n=6). Resistance was seen with amoxicillin + clavulanic acid, cefepime, ciprofloxacin and cefoperazone. The organisms were sensitive to levofloxacin, tetracycline and vancomycin.

Conclusion: Aseptic precautions taken by the healthcare personnel will bring down the infections and curb the spread of multi-drug resistant hospital acquired infections.


Horan TC, Andrus M, Dudeck MA. CDC/NHSN surveillance definition of health care-associated infection and criteria for specific types of infections in the acute care setting. Am J Infect Control 2008; 36: 309-332.

Yazan Haddadin; Hariharan Regunath. Central Line Associated Blood Stream Infections (CLABSI). Statpearls; 2017, 28th March.

Gahlot R, Nigam C, Kumar V, Gupta M. Catheter related bloodstream infections in ICU: a study from North India. Int J Infect Control 2013;v9i2;1-3

Chopdekar K, Chande C, Chavan S, et al. Central venous catheter-related blood stream infection rate in critical care units in a tertiary care, teaching hospital in Mumbai. Indian J Med Microbiology 2011; 29:169-171.

Pal N, Sujatha R. Extreme drug resistant A.baumannii associated with CLA-BSI in Kanpur. Int J Res Med Sci 2017; 5: 3620-5.

Abirami E et al. A study on Catheter related bloodstream infections (CRBSI) in Intensive care unit patients in a tertiary care hospital. Indian J Microbiol Res 2017; 4(2):138-143.

Raad I, Costerton W, Sabharwal U, Sacilowski M, Anaissie E, Bodey GP. Ultrastructural analysis of indwelling vascular catheters: a quantitative relationship between luminal colonization and duration of placement. The Journal of infectious diseases. 1993; 168(2):400-7.

Cobb DK, High KP, Sawyer RG, Sable CA, Adams RB, Lindley DA, et al. A controlled trial of scheduled replacement of central venous and pulmonary-artery catheters. The New England journal of medicine. 1992; 327(15):1062-8.

Apostolopoulou E. Surviellance of Device associated infection rate and Mortality in 3 greek Intensive care units. Am J crit care. 2013; 22(3);e12-e20.

Asmita A. Mehta V, Kumar A, Indira K, Suresh G. Nair, Dinesh KR, Singh SK. Risk factors for mortality in Acinetobacter calcoaceticus–baumannii Bacteraemia. Asian Pacific Journal of Tropical Biomedicine. 2012:S1852-S7.

Barsanti MC, Woetje KF. Infection prevention in the intensive care unit. Infect DisClin N Am.2009; 23: 703–25. [PubMed] [Google Scholar]

Sharif A, Emami A, Mazouchi T, Mosavi S, Sharif M, Verdi J, et al. Venous catheter infection of patients admitted to ICU and its related factors. KAUMS Journal (FEYZ) 2002; 6:53–8.

Ali J, Kebede Y: Frequency of isolation and antimicrobial susceptibility pattern of bacterial isolation from blood culture in Gondar University Hospital. Ethio Med J. 2008, 46(2):155-161.

Arora U, Devi P. Bacterial profile of blood stream infections and antibiotic resistance pattern of isolates. J K Sci 2007; 9: 186-190.

Sharma M, Goel N, Chaudhary U, Aggarwal R, Arora DR. Bacteraemia in children. Indian J Pediatr 2002; 69:1029-32.

Grothe C, da Silva Belasco AG, de CássiaBittencourt AR, Vianna LA, de Castro CintraSesso R, Barbosa DA. Incidence of bloodstream infectionamong patients on hemodialysis by central venous catheter. Rev Latino-am Enfermagem. 2010; 18: 73–80.

Ninama A, Golia S, Bohra S, Central line blood stream infection in intensive care unit: Importance of the care bundle, Int J Med Microbiol Trop Dis 2019;5(1):1-3

Wasihun, A.G., Wlekidan, L.N., Gebremariam, S.A., et al. (2015) Bacteriological Profile and Antimicrobial Susceptibility Patterns of Blood Culture Isolates among Febrile Patients in Mekelle Hospital, Northern Ethiopia. Springer Plus, 4, 314.

Soriano A, Marco F, Martinez JA, Pisos E, Almela M, Dimova VP et al (2008) Influence of vancomycin minimum inhibitory concentration on the treatment of methicillin-resistant S. aureus bacteremia. Clin Infect Dis 46:193–200.

Gill MK, Sharma S. Bacteriological profile and antibiotic resistance pattern in blood stream infection in critical care units of a tertiary care hospital in North India. Indian J Microbiol Res 2016; 3(3):270-274.

Jia L, Yu H, Lu J, Zhang Y, Cai Y, Liu Y, et al. [Epidemiological characteristics and risk factors for patients with catheter-related bloodstream infections in intensive care unit]. Zhonghua yi xue za zhi. 2015; 95(9):654-8.

Deliberato RO, Marra AR, Correa TD, Martino MD, Correa L, Dos Santos OF, et al. Catheter related bloodstream infection (CR-BSI) in ICU patients: making the decision to remove or not to remove the central venous catheter. PLoS One. 2012; 7(3):e32687.

Mansur FJ, Barai L, Karim MM, Haq JA, Fatema K, Faruq MO. Intravascular catheter related infections and antimicrobial susceptibility pattern of isolated bacteria in a tertiary care hospital of Bangladesh. Indian J Med Microbiol 2014; 32:68-71.

McGee DC, Gould MK. Preventing complications of central venous catheterization. N Engl J Med 2003; 348:1123-1133.




How to Cite

Rezia, R. A., Vijendra, R., & Gopi, A. (2020). A clinical study of patients with central venous catheter associated bloodstream infections in a tertiary care hospital. International Journal of Basic & Clinical Pharmacology, 9(11), 1665–1670. https://doi.org/10.18203/2319-2003.ijbcp20204491



Original Research Articles