DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20164110

Evaluation of anti-inflammatory effect of aqueous extract of Aloe vera in Albino rats

Devarsi Choudhury, Dolly Roy

Abstract


Background: The study evaluated the anti-inflammatory effect of the aqueous extract of Aloe vera [AEAV] leaves. AEAV was prepared by the Soxhlet apparatus and 500mg/kg body weight was used.

Methods: To screen for acute inflammation by carrageenan induced rat paw edema method, eighteen healthy albino rats weighing 150-200 gm each were used. They were divided in three groups with six animals in each group. At the 1st hr, 2nd hour, 3rd hour and the 4th hour, the test drug i.e. AEAV [aqueous extract of Aloe vera] showed 26.92%, 41.6%, 44.44% and 48.21 % inhibition of edema formation, whereas the standard drug Indomethacin [10 mg/kg] showed 53.85%, 61.11%, 66.67% and 71.43% inhibition respectively. Reduction of paw edema formation was highly significant in both Aloe vera and Indomethacin.

Results: In Granuloma pouch method for subacute inflammation, the chemical used was 1 ml of 20 % carrageenan suspension in sesame oil. On the 5th day pouch is opened, exudates measured. Both Aloe vera and Indomethacin showed highly significant inhibition of exudate formation.

Conclusions: In Adjuvant arthritis method for chronic inflammation, paw edema of the injected and the non-injected were measured on the 5th day and the 21st day respectively. Arthritic index was measured on the 21st day. These results were highly significant for AEAV and Indomethacin in comparison with the control. The results for the arthritic index were highly significant for both Aloe vera and Indomethacin. It is easily illustrated that the herbal plant Aloe vera at the dose of 500mg/kg demonstrated significant anti-inflammatory effect against all the experimental methods on inflammation.


Keywords


Aloe vera, AEAV, Carrageenan, Granuloma, Inflammation

Full Text:

PDF

References


Kumar V, Abbas AK, Aster JC. Inflammation and repair. Robbins and Cotran Pathologic Basis of Disease. 9th edition. New Delhi: Elsevier; 2014:69-111.

Libby P. Inflammatory Mechanisms: The Molecular Basis of Inflammation and Disease. Nutrition Reviews. 2007;65(12):S140-6.

LM Buja, Krueger GRF. Netters Illustrated Human Pathology. 2ndedition. Philadelphia: Elsevier; 2014:5-6.

Balmuth D, Ringer N. 24 Safe and Effective Herbs to Know, Grow, and Use Rosemary Gladstar’s Medicinal Herbs. United States: Storey Publishing; 2012:103-06.

Mwale M, Masika PJ. Analgesic and anti-inflammatory activities of Aloe ferox Mill. Aqueous extract. African Journal of Pharmacy and Pharmacology. 2010;4(6):291-7.

Steenkamp V, Stewart MJ. Medicinal applications and toxicological activities of Aloe products. Pharm. Biol. 2007;45:411-20.

WHO monographs on selected medicinal plants. Health Organization Geneva. 1999;1:33-42.

Vane JR, Botting RM. New insights into the mode of action of anti-inflammatory drugs. Inflammation Res. 1995;44:1-10.

Saritha V, Anilakumar KR. Toxicological evaluation of methanol extract of Aloe vera in rats. International Journal on Pharmaceutical and Biomedical Research. 2010;1(5):142-9.

Subramani V, Kamaraj M, Ramachandran B, Jeyakumar JJ. Study of trace metals concentration and antimicrobial properties of tropical Aloe vera plant from Southern India. Int J of Phy. 2014;4(3):89-91.

Gerhard Vogel H. Antiarthrotic and immunomodulatory activity. Drug discovery and evaluation. Second completely revised updated and enlarged edition. Germany: Springer publication; 2002:802-805.

Dutta S, Das S. A study of the anti-inflammatory effect of the leaves of Psidium guajava Linn. on experimental animal models. Pharmacognosy Research. 2010;2(5):313-7.

Mujumdar AM, Misar AV. Anti-inflammatory activity of Jatropha curcas roots in mice and rats. J of Eth. 2004;90:11-5.

Agus S. The Potency of Piperine as Antiinflammatory and Analgesic in rats and mice. Folia Med Indonesiana. 2005;41:190-4.

Agrawal SS, Paridhavi M. Screening methods used for herbal drugs. Herbal drug technology. Hyderabad: universities press; 2007:583-7.

Bonney RJ. Obtaining mononuclear phagocytes from granulomas. Methods for Studying Mononuclear Phagocytes. Dolph Adams. London: Academic press; 1981:112-113.

Hambleton P, Miller P. Studies on carrageenin air pouch inflammation in the rat. The British Journal of Experimental Pathology. 1989;70:425-33.

Fukuhara I, Tsurufuji S. The effect of locally injected anti-inflammatory drugs on Carrageenan induced granuloma in rats. Biochemical Pharmacology. 1969;18:475-84.

Bhattacharjee S, Paul S, Dutta S, Chaudhuri TK. Anti-inflammatory and protective properties of aloe vera leaf crude gel in carrageenan induced acute inflammatory rat models. Int J Pharm Sci. 2014;(9):368-71.

Geboes L, Klerck BD, Balen MV, Kelchtermans H, Mitera T, Boon L, et al. Freund’s Complete Adjuvant Induces Arthritis in Mice Lacking a Functional Interferon- Receptor by Triggering Tumor Necrosis Factor-Driven Osteoclastogenesis. Arthritis and Rheumatism. 2007;56(8):2595-607.

Kshirsagar AD, Panchal PV, Harle UN, Nanda RK, Shaikh HM. Anti-Inflammatory and Antiarthritic Activity of Anthraquinone Derivatives in Rodents. International Journal of Inflammation. 2014:1-12.

Egesie UG, Chima KE, Galam NZ. Anti-inflammatory and Analgesic Effects of AEAV [Aloe barbadensis] in Rats. Afr. J. Biomed. Res. 2011;14:209-12.

Derbe T, Yilma B, Badasa S. Investigation of Anti-Bacterial And Anti-Oxidant Activities Of Aloe Vera Plant At Arba Minch, Southern Ethiopia. World J of Pha and Pha Sci. 2015;4(01):185-95.

Devaraj A, Karpagam T. Evaluation of anti-inflammatory activity and analgesic effect of Aloe Vera leaf extract in rats. Int Res J of Pha. 2011;2(3):103-10.

Bhuvana KB, Hema NG. An Evaluation of Aloe Vera for its Acute Anti-inflammatory Activity in rat peritonitis model. World J of Pha Res. 2014;3(3):4508-19.