Screening of liver protective effect of ethanolic leaf extract of Callicarpa lanata (EECL) in ethanol induced hepatotoxicity in wister rats


  • Zulfkar Latief Qadrie Department of Pharmacology and Toxicology, Pratishta Institute of Pharmaceuticals Sciences, Suryapet, Hyderabad, Telangana, India
  • Rajkapoor Balasubramanium Department of Pharmacology, Faculty of Medicine, Sebha University, Sebha, Libya
  • Shakil U. Rehman Department of Pharmacology, Govt. Medical College, Baramulla, Jammu and Kashmir, India



Callicarpa lanata, Ethanol, Hepatotoxicity, Hepatocytes, Silymarin


Background: The ethanolic extract of Callicarpa lanata (EECL), belonging to the family Verbenaceae, were studied for hepatoprotective activity in Wister rats with liver damage induced by ethanol.

Methods: Ethanol treated rats showed significant increase in the levels of serum enzyme activities, total bilirubin and reduction in total proteins reflecting the liver injury caused by ethanol. EECL, at a dose of 400 and 200mg/kg body weight exhibited hepatoprotective effect by lowering the Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Alkaline Phosphate (SALP), Gama Glutamyl Transpeptidase (GGTP), total Bilirubin to a significant extent and also significantly increased the levels of total protein in a dose dependent manner.

Results: The results were highly significant at dose level of 400mg/kg body weight (p <0.01) and significant at dose level of 200mg/kg body weight (p <0.05). The effects of EECL at both levels were comparable with standard drug silymarin. The hepatoprotective activity was also supported by histopathological studies of liver tissue.

Conclusions: In-vivo hepatoprotective activity of ethanolic leaf extract of Callicarpa lanata (EECL) against Ethanol induced acute liver injury in rats showed significant results in a dose dependent manner.


Muhammad Habib-ur-Rehman, Zafar Hussain, Fakhar Hameed. Protection against Ethanol induced Hepatotoxicity by Silymarin in Albino Rats. Ann Pak Inst Med Sci. 2009; 5(4):206-10.

Muhammad Habib-ur-Rehman, Mohammad Tahir, Khalid P. Lone and Waqas Sami. Ethanol induced Hepatotoxicity in Albino Rats. J Coll Physicians Surg Pak. 2011;21(10):642-43.

Saravanan N, Nalini N. Antioxidant effect of Hemidesmus indicus on ethanol induced hepatotoxicity in rats. J Med Food. 2007;10:675-82.

Ronis MJ, Hakkak R, Korourian S, Albano E, Yoon S, Ingelman-Sundberg M, et al. Alcoholic liver disease in rat fed ethanol as part of oral or intragastric low-carbohydrate liquid diets. Exp Biol Med. 2004; 229:351-60.

Rajesh MG, Latha MS. Hepatoprotection by Elephantopus scaber Linn. in CCl4-induced liver injury. Indian J Physiol Pharmacol. 2001 Oct;45(4):481-6.

Anandan R, Rekha RD, Devaki T. Protective effect of Picrorhiza kurroa on mitochondrial glutathione antioxidant system in D-galactosamine-induced hepatitis in rats. Curr Sci. 1999 Jun 25;76(12):1543-5.

Qadrie ZL, Anandan R, Jacob B, Ashraf H. Liver Protective Activity of Indoneesiella Echioides Against Carbon Tetrachloride (CCl4)-Induced Hepatotoxicity In Rats. Pharmacologyonline. 2011;2:416-29.

Nadkarni AK, Nadkarni KM. Indian Materia Medica. Bombay: Popular Prakashan. 1975;1:252-53.

Ghani A. Medicinal Plants of Bangladesh with Chemical Constituents and Uses. 2nd Edn. Asiatic Society of Bangladesh; 1998: 266-67.

Asolkar LV, Kakkar and Chakre. Second Supplement to Glossary of Indian Medicinal Plants with active principles. Part-I (A-K), CSIR, New Delhi. 1992:129-30.

Zulfkar Q, Balasubramanian R, Kavimani S. Acute and Subacute Toxicity of Callicarpa linata leaf Extracts in Rats. Int J Pharm Research Sci. 2013 Nov;2(11):1095-104.

Kokate CK, Purohit AP, Gokhale SB. A Text Book of Pharmacognosy. 28th Ed., Nirali Prakashan. 2004:1-19.

Leo MA, Lieber CS. Hepatic fibrosis after long-term administration of ethanol and moderate vitamin A Supplementation in the rat. Hepatol. 1983;3(1):111.

Arulkumaran KS, Rajasekaran A, Ramasamy R, Jegadeesan M, Kavimani S, Somasundaram A. Cassia roxburghii seeds protect liver against toxic effects of ethanol and carbontetrachloride in rats. Int J Pharm Tech Res. 2009;1(2):273-6.

Dahiru D, Obidoa O. Evaluation of the antioxidant effects of Ziziphus mauritiana lam. leaf extracts against chronic ethanol-induced hepatotoxicity in rat liver. African J TraditionalComplementary Alternative Med. 2008;5(1):39-45.

Saka WA, Akhigbe RE, Ishola OS, Ashamu EA, Olayemi OT, et al. Hepato therapeutic effect of Aloevera in alcohol induced Hepatic damage. Pak J Bio Sci. 2011;14(14):742-46.

Vetriselvan S, Subasini U, Victor Rajamanickam C, Thirumurugu S. Hepatoprotective activity of Andrographis paniculata in ethanol induced hepatotoxicity in albino wistar rats. Pharma Globale. 2011;2(2):1-4.

Wilber KM. Bernheim Fshapiro OW. Determination of lipid peroxidation. Arch Biochem. 1949;24:305-10.

Kono Y. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophysics. 1978 Feb 1;186(1):189-95.

Hugo EB. Oxidoreductase activity on groups other than CHOH: Catalase. In:Colowick SP, Kaplan NO, Packer L, eds. Methods in Enzymology. London: Academic Press. 1984:121-125.

Rani P, Unni KM, Karthikeyan J. Evaluation of antioxidant properties of berries. Indian J Clin Biochem. 2004 Jul 1;19(2):103-10.

Alquasoumi SI, Al-Dosari MS, Al-Sheikh AM, Abdel Kader MS. Evaluation of the hepatoprotective effect of Fumaric parviflora and Momordica balsamine from Saudi flock medicine against experimentally induced liver injury in rats. Res J Med Plant. 2009;3:9-15.

Srilakshimi SV, Vijayan P, Vsantha Raj P, Dhanaraj SA, et al. Hepatoprotective properties of Caesalpinia sappan Linn. heartwood on carbon tetrachloride induced toxicity. Ind J Exp Biol. 2010;48:905-10.

Leo MA, Arai M, Sato M, Lieber CS. Hepatotoxicity of vitamin A and ethanol in the rat. Gastroenterol. 1982 Feb 1;82(2):194-205.

Zakhari S, Li TK. Determinants of alcohol use and abuse: impact of quantity and frequency patterns on liver disease. Hepatol. 2007 Dec;46(6):2032-9.

Zhou Z, Wang L, Song Z, Lambert JC, McClain CJ, Kang YJ. A critical involvement of oxidative stress in acute alcohol-induced hepatic TNF-α production. Am J Pathol. 2003 Sep 1;163(3):1137-46.

Thurman RG. II. Alcoholic liver injury involves activation of Kupffer cells by endotoxin. Am J Physiol Gastrointestinal Liver Physiol. 1998 Oct 1;275(4):G605-11.

Tsukamoto H, Takei Y, McClain CJ, Joshi‐Barve S, Hill D, Schmidt J, et al. How is the liver primed or sensitized for alcoholic liver disease?. Alcoholism Clin Experiment Res. 2001 May 1;25:171S-81S.

Baraona E, Leo MA, Borowsky SA, Lieber CS. Alcoholic hepatomegaly: accumulation of protein in the liver. Science. 1975 Nov 21;190(4216):794-5.

Baraona E, Leo MA, Borowsky SA, Lieber CS. Pathogenesis of alcohol-induced accumulation of protein in the liver. J Clin Investigation. 1977 Sep 1;60(3):546-54.

Yue M, Ni Q, Yu CH, Ren KM, Chen WX, Li YM. Transient elevation of hepatic enzymes in volunteers after intake of alcohol. HBPD INT. 2006 Feb;5(1):52-5.

Mukherjee PK. Quality control of herbal drugs: an approach to evaluation of botanicals. Business Horizons. 2002:531.




How to Cite

Qadrie, Z. L., Balasubramanium, R., & Rehman, S. U. (2019). Screening of liver protective effect of ethanolic leaf extract of Callicarpa lanata (EECL) in ethanol induced hepatotoxicity in wister rats. International Journal of Basic & Clinical Pharmacology, 8(3), 595–600.



Original Research Articles