Adequacy of phosphodiesterase inhibitor in prevention and treatment of LPS induced organ failure in BALB/c mice

Ammara Khan; Ayesha Afzal; Hammad A. Butt; Akbar Waheed

doi:10.18203/2319-2003.ijbcp20190664

Authors

Ammara Khan Department of Pharmacology, Therapeutics, Nawaz Sharif Medical College, University of Gujrat. Hafiz Hayat Campus. Gujrat, Pakistan
Ayesha Afzal Department of Pharmacology, Therapeutics, Wah Medical College, Wah Cantt, Wah, Pakistan
Hammad A. Butt Department of Pharmacology and Therapeutics, Combined Military Hospital Kharian, Pakistan
Akbar Waheed Department of Pharmacology and Therapeutics, Islamic International Medical College, Riphah International University, Islamabad, Pakistan

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20190664

Keywords:

Endotoxin, Gram negative sepsis, Hepatotoxicity, LPS, Pentoxifylline, Phosphodiesterase inhibitor

Abstract

Background: Even though with immense improvement and extensive understanding of pathophysiology of sepsis induced organ failure and affected population, it continues to put hundreds of people worldwide to eternal sleep due to lack of targeted therapy. Newer treatment modalities is the dire need of time. The present study was aimed to ascertain the adequacy of phosphodiesterases inhibitor - pentoxifylline (75mg/kg i.p) in endotoxin/LPS induced hepatotoxicity in BALB/c mice.

Methods: The number of animals in each group was six. Endotoxin/lipopolysaccharides induced hepatotoxicity was reproduced in mice by giving lipopolysaccharide of serotype E. coli intraperitoneally. To ascertain the Preventive role, pentoxifylline was administered forehand LPS injection whereas therapeutic potential adjuged via post LPS delivering. The extent of liver damage was evaluated through serum alanine aminotransferases (ALT) and aspartate aminotransferase (AST) estimation along with histopathological examination of liver tissue.

Results: Results set forth that serum ALT, AST levels and histological alteration abated considerably (p ≤0.05) both in animals subjected to pentoxifylline pre and post-treatment.

Conclusions: Pentoxifylline set up promising results in endotoxin induced hepatotoxicity and can be used therapeutic adjuncts to conventional treatment strategies in sepsis induced liver failure.

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