Anti-tumor effect of fruit rind of Myristica malabarica in an Ehrlich ascites carcinoma model
Keywords:Anti-tumor, Ehrlich ascites carcinoma, Malabaricones, Myristica malabarica, Rampatri
Background: Among the various modalities of anti-cancer treatment, cancer chemotherapy plays a very vital role. The alarming side effects being its main drawback leads to relentless research for newer agents. A new natural agent with promising anti-cancer properties from in-vitro studies leads to this study. Here we have evaluated the anti-tumor activity of a crude extract of fruit rind of Myristica malabarica in an Ehrlich ascites carcinoma model in mice.
Methods: A murine model of cancer was established with i.p. inoculation of Ehrlich Ascites carcinoma (EAC) cells; animals were divided into five groups (including normal control) to observe the inhibitory effect of a crude extract of the fruit rind of Myristica malabarica/rampatri (0-100mg/kg b.w. i.p.) as compared with methotrexate (0.4mg/kg bw., i.p.). Blood and ascitic fluid were collected on the 10th day for analysis.
Results: In the EAC model, there was an increase in tumor volume, tumor weight, and tumor packed cell volume, which was decreased by rampatri (50 and 100mg/kg bw) along with an increase in the mean survival time (MST). Rampatri caused minimal alterations in hematological parameters, renal functions remained unchanged but an increase in hepatic SGOT was demonstrated.
Conclusions: The crude extract of rampatri (containing Malabaricones) exhibited significant anti-tumor activity with minimal effect on hematological and renal functions.
Morita T, Jinno K, Kawagishi H, Arimoto Y, Suganuma H, Inakuma T, et al. Hepatoprotective effect of Myristica from nutmeg (Myristica fragrant) on lipopolysaccharide/d-galactosamine-induced liver injury. J Agric Food Chem. 2003;51:1560-5.
Banerjee D, Bandyopadhyay SK, Bauri AK, Chattopadhyay S. Healing properties of malabaricone B and malabaricone C, against indomethacin-induced gastric ulceration and mechanism of action. Eur J Pharmacol. 2008;578:300-12.
Sen R, Bauri AK, Chattopadhyay S, Chatterjee M. Antipromastigote activity of the malabaricones of Myristica malabarica(Rampatri) Phytother Res. 2007;21:592-5.
Manna A, Saha P, Sarkar A, Mukhopadhyay D, Bauri AK, Kumar D, et al. Malabaricone-A induces a redox imbalance that mediates apoptosis in U937 cell line. PLoS One. 2012;7:e36938.
Patro BS, Bauri AK, Mishra S, Chattopadhyay S. Antioxidant activity of Myristica malabarica extracts and their constituents. J Agric Food Chem. 2005;53:6912-8.
Tyagi M, Bhattacharyya R, Bauri AK, Patro BS, Chattopadhyay S. DNA damage dependent activation of checkpoint kinase-1 and mitogen-activated protein kinase-p38 are required in malabaricone C-induced mitochondrial cell death. Biochim Biophys Acta. 2014;1840:1014-27.
Bala A, Kar B, Haldar PK, Mazumder UK, Bera S. Evaluation of anticancer activity of Cleome gynandraon Ehrlich's Carcinoma treated mice. J Ethnopharm. 2010;129:131-4.
Manna A, Bauri AK, Chattopadhyay S, Chatterjee M. Effectiveness of Malabaricone-A in a multidrug resistant cell line is mediated via generation of redox imbalance. Anticancer Agents Med Chem. 2015;15:1156-63.
Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M. The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance. Phytomed. 2015;22:713-23.
Manna A, Chattopadhyay S, Chatterjee M. Plant derived pro-oxidants as potential anti-cancer therapeutics. 2016; Studies in Natural Products Chemistry; Atta-ur- Rahmaned (Bioactive Natural Products), Elsevier, Amsterdam; 2016;81-92.
Haldar PK, Kar B, Bala A, Bhattacharya S, Mazumder UK. Antitumor activity of Sansevieria roxburghiana rhizome against Ehrlich ascites carcinoma in mice. Pharm Biol. 2010;48:1337.
KunduSen S, Gupta M, Mazumder UK, Haldar PK, Saha P, Bala A et al. Antitumor activity of Citrus maxima (Burm.) Merr. Leaves in Ehrlich’s Ascites Carcinoma Cell-treated mice. ISRN Pharmacol. 2011;138737.
Ozaslan M, Karagoz ID, Kilic IH, Guldur ME. Ehrlich ascites carcinoma. J Home. 2011;10:13.
Manjunatha BK, Mankani KL, Mukunda SK, Divakara R, Sridar BK and Paul K. Antioxidant and hepatoprotective effect of Myristica malabarica seed aril extracts on carbon tetrachloride induced hepatic damage. Global J Biotechnol Biochem. 2011;6:25-30.