Activity of Tulsi leaves (Ocimum sanctum linn) in protecting gastric ulcer in rats by cold restrain method

Ayesha Vaseem, Mazher Ali, Khuteja Afshan


Background: Tulsi (Ocimum sanctum L.) common household plant, a potent adaptogen has been used for its medicinal property for many years. It is known in Ayurveda as Incomparable one, Mother of medicine of Nature and The Queen of Herbs. The present study evaluated the stress induced anti-ulcer effect of tulsi leaves in albino rats.

Methods: Albino rats were randomly, allocated to different experimental groups and aqueous leaf extract of Tulsi (Ocimum sanctum) is given for 7 days. Gastric ulcers were induced by cold restrain methods and results were compared with control using distilled water and standard drug Ranitidine.

Results: The Anti-ulcer effect of aqueous extract of Tulsi was significant in a dose dependent manner with cold restraint model. The percentage of ulcer protection of Tulsi leaf extract at dose 100mg/kg is 68.85% and at dose of 200mg/kg showed 65.66% and the standard drug Ranitidine showed 78.23% ulcer protection which is significant (p <0.05).

Conclusions: The study showed that when Tulsi (Ocimum sanctum) is given at dose 100-200mg/kg for 7 days i.e., pre-treatment in rats for anti-ulcer effect, it caused a significant reduction in the ulcer scores and ulcer index when compared with control group, as evidenced by the gross appearance and histological findings of gastric mucosa in rats. There were few signs of mucosal injury and the percentage of damage was less compared to control group. Serosal surface revealed very few dilated blood vessels and petechial hemorrhages and mucosal surface had few ulcers of varying sizes.


Cold restrain ulcers, Peptic ulcer, Tulsi

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Hoogerwerf WA, Pasricha PJ. Agents used for control of gastric acidity and treatment of peptic ulcers and gastroesophageal reflux disease. In: Hardman JG, Limbird LE, Goodman Gilaman A, editors. Goodman and Gilman the Pharmacological Basis of Therapeutics. 10th Ed. New York: Mc Graw-Hill; 2001:1005-1019.

Falcao HS, Mariath IR, Diniz MFFM, Batista LM, Barbosa JM. Filho, Phytomed. 2008;15:132-46.

Bijlani RL, Vempati RP, Yadav RK, Ray RB, Gupta V, Sharma R, et al. A brief but comprehensive lifestyle education program based on yoga reduces risk factors for cardiovascular disease and diabetes mellitus. Journal of Alternative & Complementary Medicine. 2005 Apr 1;11(2):267-74.

Qiu BS, Cho CH, Ogle CW. The influence of chronic nicotine treatment on stress-induced gastric ulceration and emptying rate in rats. Experientia. 1992;48:389-91.

Perry MA, Wadhwa S, Parks DA, Pickard W, Granger DN. Role of oxygen radicals in ischemia-induced lesions in the cat stomach. Gastroenterology. 1986 Feb 28;90(2):362-7.

Jana U, Bhattacharyya D, Bandopadhyay S, Pandit S, Debnath PK, Sur TK. Antiulcer activity of digitrall: A polyherbal drug in rats. 2005;37:406-7.

Pihan G, Regillo C, Szabo S. Free radicals and lipid peroxidation in ethanol and aspirin-induced gastric mucosal injury. Dig Dis Sci. 1987;32:1395-401.

Atal CK, Kapoor BM. Cultivation and utilization of medicinal plants (Eds. PID CSIR), 1989.

Vincent G, Glavin G, Rutkowski J, Pare W. Body orientation, food deprivation and potentiation of restraint induced gastric lesions. Gastroenterologie clinique et biologique. 1977;1(6-7):539.

Nagaraja HS, Jeganathan PS. Forced Swimming stress induced alterations in ingestive behavior in rats. Indain journal of Physiology Pharmacology. 2003;41(1):94-100.

Sahakari SD, Abraham ME, Mascarenhas JF. Effect of stress on maternal behaviour. Indian journal of physiology and pharmacology. 1989 Apr 1;33(2):93-6.

Kelm MA, Nair MG, Strasburg GM, DeWitt DL. Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum Linn. Phytomedicine. 2000;7:7-13.

Shishodia S, Majumdar S, Banerjee S, Aggarwal BB. Urosolic acidinhibits nuclear factor-kappaB activation induced by carcinogenic agents through suppression of IkappaBalpha kinase and p65 phosphorylation: Correlation with down-regulation of cyclooxygenase 2, matrix metalloproteinase 9, and cyclin D1. Cancer Res. 2003;63:4375-83.

Jaggi RK, Madaan R, Singh B. Anticonvulsant potential of holy basil, Ocimum sanctum Linn., and its cultures. Indian J Exp Biol. 2003;41;1329-33.

Dharmani P, Kuchibhotla VK, Maurya R, Srivastava S, Sharma S, Patil G. Evaluation of anti-ulcerogenic and ulcer-healing properties of Ocimum sanctum Linn. J Ethnophamacol. 2004;93:197-206.

Pandey G, Madhuri S. Pharmacological activities of Ocimum sanctum (tulsi): a review. Int J Pharm Sci Rev Res. 2010;5:61-6.

Cohen MM. Tulsi - Ocimum sanctum: A herb for all reasons. Journal of Ayurveda and Integrative Medicine. 2014;5(4):251-9.