Effect of ethyl acetate extract of Melothria perpusilla on dexamethasone induced hyperglycemia in albino rats
Keywords:Diabetes mellitus, Dexamethasone, Glibenclamide, Melothria perpusilla
Background: Diabetes mellitus is a group of heterogeneous disorders in which carbohydrate metabolism is reduced while that of proteins and lipids is increased. Safety and tolerability factors limit the clinical use of anti diabetic drugs. The objectives of the study were to evaluate the effect of ethyl acetate extract of Melothria perpusilla (EAEMP) on dexamethasone induced hyperglycemia in albino rats.
Methods: A set of six animals each weighing 110-150g were used for the experimental study. Successive tests were conducted on the same set of animals after a period of 10 days in between the drug administration. Blood was collected from the orbital sinus and fasting blood glucose levels were measured; 2% gum acacia suspension was administered in all the six animals followed by dexamethasone (0.5ml/100g) intraperitoneal injection. Blood glucose concentrations were estimated in the blood samples collected at 1h and 2h after the administration of dexamethasone administration. With the same set of animals, similar tests were repeated with the test dose of 250 mg/kg and 500 mg/kg of the ethyl acetate extract of Melothria perpusilla and glibenclamide [0.5mg/kg per oral (p.o.)].
Results: Scientific data were analysed by Kruskal Wallis test. Ethyl acetate extract of Melothria perpusilla produced a significant reduction of blood glucose level when compared with control and standard.
Conclusions: Treatment with Melothria perpusilla improves hyperglycaemia probably by inhibiting gluconeogenesis.
Sharma HL, Sharma KK. Principles of Pharmacology. 2nd ed. Delhi: Paras Medical Publishers; 2011:630.
Carla J, Greenbaum, Leonard C, Harrison. Diabetes: translating research into practice. Informa health care; 2008:1-2.
Mukesh R, Namita P. Medicinal plants with antidiabetic potential-A review. American-Eurasian J Agric Environ Sci. 2013;13(1):81-94.
Dey L, Attele AS, Yuan CS. Alternative therapies for type 2 diabetes. Altern Med Rev. 2002;7:45-58.
Yaheya M, Ismail M. World Appl Sci J. 2009;7(10):1231-4.
Kiritikan KR, Basu BD. Melothria perpusilla Cogn. In: Blatter E, Caius JF, Mlaskar KS, editors. Indian Medicinal Plant. 2nd ed. Dehradun: International Book Distributors; 1987:161-2.
Leisangthem S, Sharma LD. Study of some important medicinal plants found in Imphal-East District, Manipur, India. Int J Scien Res Pub. 2014;4(9):3.
Sinha SC. Medicinal plants of Manipur, Imphal (Manipur): Manipur Association for Science and Society (MASS); 1996.
Langoljam RD, Kongbrailatpam BD, Loitongjam WS. Sterols and flavonol glycosides from Melothria perpusilla Cogn. Indian J Chem. 2005;44(B):1291-4.
Beckett AH, Stenlake JB. Solvent extraction methods practical pharmaceutical chemistry. 3rd ed. London: The Anthlone Press; 1975.
Chattopadhyay RR, Sarkar SK, Ganguly S, Banerjee RN, Basu TK. Indian J Physiol Pharmacol. 1991;35(3):45-51.
Brahmachari HD, Augusti KT. Isolation of orally effective hypoglycaemic compounds from Ficus bengalensis Linn. Indian J PhysiolPharmacol. 1964;8:60-4.
Knevel AM, Digangi FE. Jenkin’s Quantitative Pharmaceutical Chemistry. 7th ed. New York: Mc Graw Hill Book Co; 1977.
OECD Test Guideline 423: Acute oral toxicity- Acute toxic class method. Available from: http://iccvam.niehs.nih.gov/SuppDocs/FedDocs/OECD/OECD_GL423.pdf. Accessed on Dec 21, 2016.
Md. Shalam, Harish MS, Farhana SA. Prevention of dexamethasone and fructose- induced insulin resistance in rats by SH-01 D, a herbal preparation. Indian J Pharmacol. 2006;38:419-22.
Sharma HL, Sharma KK. Principles of Pharmacology. 2nd ed. Delhi: Paras Medical Publishers; 2011:565.
Bourne HR, Zastro MV. Drug receptors and Pharmacodynamics, In: Katzung BG, editor. Basic and Clinical pharmacology. 8th ed. New York: McGraw Hill; 2001:9-34.
Schimmer BP, Parker KL. Adrenocorticotropic hormone; Adrenocorticol steroids and their synthetic analogs; Inhibitors of the synthesis and actions of Adrenocortical hormones. In: Hardman JG, Limbard LE, Gilman AG, editors. Goodman and Gillman’s the Pharmacological Basis of Therapeutics. 10th ed. New York: Mc Graw Hill; 2001:1649-1677.