Evaluation of hepatoprotective activity of Boerhaavia diffusa against carbon tetrachloride induced liver toxicity in albino rats
Keywords:Carbon tetrachloride, Hepatoprotective activity, Boerhaavia diffusa, Silymarin
Background: Modern allopathic medicine has very little to offer for the treatment of liver disorders in spite of consistent effort for new drug discovery. Hence, this study was conducted to elucidate the hepatoprotective activity of aqueous extract of traditional medicinal plant Boerhaavia diffusa against carbon tetrachloride (CCl4) induced toxicity in male albino rats.
Methods: The hepatoprotective effect of the aqueous extracts of B. diffusa was evaluated by biochemical parameters such as serum alanine transferases (ALT), serum asparate transferases (AST), alkaline phosphatase (ALP), total serum bilirubin, and serum protein, and confirmed by histopathology of liver. The toxicant CCl4 was used to induce hepatotoxicity and silymarin were used as control drug. The aqueous extracts of B. diffusa were administered at the doses of 250 mg/kg/day and 500 mg/kg/day orally for 4 days. One-way analysis of variance was used for the statistical analysis of data. A probability value of p<0.05 was considered as significant.
Results: Administration of B. diffusa at doses 250 and 500 mg/kg orally demonstrated hepatoprotective activity by preventing the increase of ALT, AST, ALP, and serum bilirubin and also confirmed by histopathology of the liver. The results were comparable to that of silymarin®.
Conclusion: The results of this study confirmed the hepatoprotective activity of aqueous extracts of B. diffusa at doses of 250 mg/kg/and 500 mg/kg/against CCl4 induced hepatotoxicity in rats. However, the dose adjustments may be necessary to optimize the similar hepatoprotective efficacy in clinical settings.
Girish C, Pradhan SC. Indian herbal medicines in the treatment of liver diseases: problems and promises. Fundam Clin Pharmacol. 2012;26(2):180-9.
Chandan BK, Sharma AK, Anand KK. Boerhaavia diffusa: a study of its hepatoprotective activity. J Ethnopharmacol. 1991;31(3):299-307.
Rawat AK, Mehrotra S, Tripathi SC, Shome U. Hepatoprotective activity of Boerhaavia diffusa L. roots – a popular Indian ethnomedicine. J Ethnopharmacol. 1997;56(1):61-6.
Anantha KC, Siva RC, Manohar RA. Hepatoprotective effect of biherbal ethanolic extract against paracetamol-induced hepatic damage in albino rats. J Ayurveda Integr Med. 2012;3(4):198-203.
Parveen R, Baboota S, Ali J, Ahuja A, Vasudev SS, Ahmad S. Effects of silymarin nanoemulsion against carbon tetrachloride-induced hepatic damage. Arch Pharm Res. 2011;34(5):767-74.
Das SK, Vasudevan DM. Protective effects of silymarin, a milk thistle (Silybium marianum) derivative on ethanol-induced oxidative stress in liver. Indian J Biochem Biophys. 2006;43(5):306-11.
Gupta NK, Dixit VK. Evaluation of hepatoprotective activity of Cleome viscosa Linn. extract. Indian J Pharmacol. 2009;41(1):36-40.
Lodhi G, Singh HK, Pant KK, Hussain Z. Hepatoprotective effects of Calotropis gigantea extract against carbon tetrachloride induced liver injury in rats. Acta Pharm. 2009;59(1):89-96.
Gupta NK, Dixit VK. Hepatoprotective activity of Cleome viscosa Linn. extract against thioacetamide-induced hepatotoxicity in rats. Nat Prod Res. 2009;23(14):1289-97.
Das S, Bandyopadhyay S, Ramasamy A, Mondal S. Evaluation of hepatoprotective activity of aqueous extracts of leaves of Basella alba in albino rats. Nat Prod Res. 2014:1-6.
Gomes A, Alam MA, Datta P, Bhattacharya S, Gomes A. Hepatoprotective activity of the edible snail (Bellamia bengalensis) flesh extract in carbon tetrachloride induced hepatotoxicity in rats. J Ethnopharmacol. 2011;138(1):228-32.
Nema AK, Agarwal A, Kashaw V. Hepatoprotective activity of Leptadenia reticulata stems against carbon tetrachloride-induced hepatotoxicity in rats. Indian J Pharmacol. 2011;43(3):254-7.
Ghosh N, Ghosh R, Mandal V, Mandal SC. Recent advances in herbal medicine for treatment of liver diseases. Pharm Biol. 2011;49(9):970-88.
Breikaa RM, Algandaby MM, El-Demerdash E, Abdel-Naim AB. Biochanin A protects against acute carbon tetrachloride-induced hepatotoxicity in rats. Biosci Biotechnol Biochem. 2013;77(5):909-16.
Ashok Kumar BS, Lakshman K, Arun Kumar PA, Viswantha GL, Veerapur VP, Thippeswamy BS, et al. Hepatoprotective activity of methanol extract of Amaranthus caudatus Linn. against paracetamol-induced hepatic injury in rats. Zhong Xi Yi Jie He Xue Bao. 2011;9(2):194-200.
Srirama R, Deepak HB, Senthilkumar U, Ravikanth G, Gurumurthy BR, Shivanna MB, et al. Hepatoprotective activity of Indian Phyllanthus. Pharm Biol. 2012;50(8):948-53.
Saraswat B, Visen PK, Patnaik GK, Dhawan BN. Protective effect of picroliv, active constituent of Picrorhiza kurrooa, against oxytetracycline induced hepatic damage. Indian J Exp Biol. 1997;35(12):1302-5.
Tatiya AU, Surana SJ, Sutar MP, Gamit NH. Hepatoprotective effect of poly herbal formulation against various hepatotoxic agents in rats. Pharmacognosy Res. 2012;4(1):50-6.
El-Sayed el-SM, Mansour AM, Nady ME. Protective Effects of Pterostilbene against Acetaminophen-Induced Hepatotoxicity in Rats. J Biochem Mol Toxicol. 2015;29(1):35-42.
Manu KA, Leyon PV, Kuttan G. Studies on the protective effects of Boerhaavia diffusa L. against gamma radiation induced damage in mice. Integr Cancer Ther. 2007;6(4):381-8.