DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20150379

Beneficial effects of Morus alba and Azadirachta indica leaf extract based combination cream in scalding type burn injury in rats

Nitish Bhatia, Raghuveerv Sharma, Gursharan Kaur, Narinder Kaur, Gurwinder Singh, Ravi Dhawan

Abstract


Background: The present study was designed to evaluate the potential of a combination cream of aqueous extracts of leaves of Morus alba (MA) and Azadirachta indica (AI) in scalding type burn wound injury in rats.

Methods: Plant material was successively extracted and aqueous extracts were selected. Three extract based cream formulations viz. 20% w/w(MA), 20% w/w (AI), and combination cream containing 10%+10% w/w (MA+AI) were prepared. Cream base and standard anti-burn cream containing silver sulfadiazine were also used for comparison. Scalding type burn was given by pouring water at 90°C on a shaved dorsal area of 20 mm2. Deep second-degree burn injury was produced which was evaluated for next 21 days for a percentage of wound contraction and period of epithelialization. On 21st day, animals were sacrificed and histopathological slides were prepared using hematoxylin-eosin staining. Burned tissue was also screened for levels of oxidative stress using thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) estimation.

Results: There was a significant increase in the percentage of wound contraction and significant decrease in period of epithelialization in MA, AI, and MA+AI combination cream treated group as compared with control group. However, most significant results were obtained with MA+AI combination cream. Histologically, MA+AI cream treatment resulted in almost complete re-epithelialization and restructuring of the wound tissue. There was a significant rise in TBARS and decrease in GSH levels in burn injury group which was reversed to a major extent by the application of combination cream.

Conclusions: The results indicate toward the possible role of free radical scavenging potential of extracts in the Burn wound tissue healing.


Keywords


Morus alba, Azadirachta indica, Scalding, Cream, Burn injury, Free radicals

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