Beneficial effects of Morus alba and Azadirachta indica leaf extract based combination cream in scalding type burn injury in rats


  • Nitish Bhatia Department of Pharmacology, Pharmacology Research Laboratory, Khalsa College of Pharmacy, Amritsar, Punjab, India
  • Raghuveerv Sharma Department of Pharmacology, Pharmacology Research Laboratory, Khalsa College of Pharmacy, Amritsar, Punjab, India
  • Gursharan Kaur Department of Pharmacology, Pharmacology Research Laboratory, Khalsa College of Pharmacy, Amritsar, Punjab, India
  • Narinder Kaur Departmaent of Chemistry, Khalsa College of Pharmacy and Technology, Amritsar, Punjab, India
  • Gurwinder Singh Departmaent of Histopathology, Khalsa Diagnostic Laboratory, Amritsar, Punjab, India
  • Ravi Dhawan Department of Pharmacology, Pharmacology Research Laboratory, Khalsa College of Pharmacy, Amritsar, Punjab, India



Morus alba, Azadirachta indica, Scalding, Cream, Burn injury, Free radicals


Background: The present study was designed to evaluate the potential of a combination cream of aqueous extracts of leaves of Morus alba (MA) and Azadirachta indica (AI) in scalding type burn wound injury in rats.

Methods: Plant material was successively extracted and aqueous extracts were selected. Three extract based cream formulations viz. 20% w/w(MA), 20% w/w (AI), and combination cream containing 10%+10% w/w (MA+AI) were prepared. Cream base and standard anti-burn cream containing silver sulfadiazine were also used for comparison. Scalding type burn was given by pouring water at 90°C on a shaved dorsal area of 20 mm2. Deep second-degree burn injury was produced which was evaluated for next 21 days for a percentage of wound contraction and period of epithelialization. On 21st day, animals were sacrificed and histopathological slides were prepared using hematoxylin-eosin staining. Burned tissue was also screened for levels of oxidative stress using thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) estimation.

Results: There was a significant increase in the percentage of wound contraction and significant decrease in period of epithelialization in MA, AI, and MA+AI combination cream treated group as compared with control group. However, most significant results were obtained with MA+AI combination cream. Histologically, MA+AI cream treatment resulted in almost complete re-epithelialization and restructuring of the wound tissue. There was a significant rise in TBARS and decrease in GSH levels in burn injury group which was reversed to a major extent by the application of combination cream.

Conclusions: The results indicate toward the possible role of free radical scavenging potential of extracts in the Burn wound tissue healing.


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How to Cite

Bhatia, N., Sharma, R., Kaur, G., Kaur, N., Singh, G., & Dhawan, R. (2017). Beneficial effects of Morus alba and Azadirachta indica leaf extract based combination cream in scalding type burn injury in rats. International Journal of Basic & Clinical Pharmacology, 4(4), 720–728.



Original Research Articles