Beneficial effects of Morus alba and Azadirachta indica leaf extract based combination cream in scalding type burn injury in rats
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20150379Keywords:
Morus alba, Azadirachta indica, Scalding, Cream, Burn injury, Free radicalsAbstract
Background: The present study was designed to evaluate the potential of a combination cream of aqueous extracts of leaves of Morus alba (MA) and Azadirachta indica (AI) in scalding type burn wound injury in rats.
Methods: Plant material was successively extracted and aqueous extracts were selected. Three extract based cream formulations viz. 20% w/w(MA), 20% w/w (AI), and combination cream containing 10%+10% w/w (MA+AI) were prepared. Cream base and standard anti-burn cream containing silver sulfadiazine were also used for comparison. Scalding type burn was given by pouring water at 90°C on a shaved dorsal area of 20 mm2. Deep second-degree burn injury was produced which was evaluated for next 21 days for a percentage of wound contraction and period of epithelialization. On 21st day, animals were sacrificed and histopathological slides were prepared using hematoxylin-eosin staining. Burned tissue was also screened for levels of oxidative stress using thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) estimation.
Results: There was a significant increase in the percentage of wound contraction and significant decrease in period of epithelialization in MA, AI, and MA+AI combination cream treated group as compared with control group. However, most significant results were obtained with MA+AI combination cream. Histologically, MA+AI cream treatment resulted in almost complete re-epithelialization and restructuring of the wound tissue. There was a significant rise in TBARS and decrease in GSH levels in burn injury group which was reversed to a major extent by the application of combination cream.
Conclusions: The results indicate toward the possible role of free radical scavenging potential of extracts in the Burn wound tissue healing.
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References
Gore MA, Akolekar D. Evaluation of banana leaf dressing for partial thickness burn wounds. Burns. 2003;29(5):487 92.
Evers LH, Bhavsar D, Mailänder P. The biology of burn injury. Exp Dermatol. 2010;19(9):777-83.
Youn YK, LaLonde C, Demling R. The role of mediators in the response to thermal injury. World J Surg. 1992;16(1):30 6.
Saitoh D, Okada Y, Ookawara T, Yamashita H, Takahara T, Ishihara S, et al. Prevention of ongoing lipid peroxidation by wound excision and superoxide dismutase treatment in the burned rat. Am J Emerg Med. 1994;12(2):142-6.
Hosnuter M, Gürel A, Babucçu O, Armutcu F, Kargi E, Isikdemir A. The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns. 2004;30(2):121-5.
Trombly R, Tappel A. Fractionation and analysis of fluorescent products of lipid peroxidation. Lipids. 1975;10(8):441-7.
Singer AJ, Taira BR, Lin F, Lim T, Anderson R, McClain SA, et al. Curcumin reduces injury progression in a rat comb burn model. J Burn Care Res. 2011;32(1):135-42.
Khorasani G, Hosseinimehr SJ, Zamani P, Ghasemi M, Ahmadi A. The effect of saffron (Crocus sativus) extract for healing of second-degree burn wounds in rats. Keio J Med. 2008;57(4):190-5.
Bhatia N, Verma P, Sharma P, Sharma P, Tuli A, Kumar A, et al. Evaluation of burn wound healing property of orange-peel extract based cream on rabbits. Int J Pharm Innov. 2011;1(5):31-8.
Flohe RB, Traber MG. Vitamin E function and metabolism. FASEB J. 1999;13:1145-55.
Levine M. New concepts in the biology and biochemistry of ascorbic acid. N Engl J Med. 1986;314(14):892-902.
Powell SR. The antioxidant properties of zinc. J Nutr. 2000;130 5S Suppl:1447S-54.
Pacher P, Nivorozhkin A, Szabo C. Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol. Pharmacol Rev. 2006;58(1):87 114.
Reiter RJ, Tan DX, Osuna C, Gitto E. Actions of melatonin in the reduction of oxidative stress. A review. J Biomed Sci. 2000;7(6):444-58.
Grinberg L, Fibach E, Amer J, Atlas D. N-acetylcysteine amide, a novel cell-permeating thiol, restores cellular glutathione and protects human red blood cells from oxidative stress. Free Radic Biol Med. 2005;38(1):136-45.
Latha B, Babu M. The involvement of free radicals in burn injury: a review. Burns. 2001;27(4):309-17.
Kao CC, Garner WL. Acute burns. Plast Reconstr Surg. 2000;101(7):2482-93.
Iqbal S, Younas U, Sirajuddin, Chan KW, Sarfraz RA, Uddin K. Proximate composition and antioxidant potential of leaves from three varieties of Mulberry (Morus sp.): a comparative study. Int J Mol Sci. 2012;13(6):6651-64.
Singab AN, El-Beshbishy HA, Yonekawa M, Nomura T, Fukai T. Hypoglycemic effect of Egyptian Morus alba root bark extract: effect on diabetes and lipid peroxidation of streptozotocin-induced diabetic rats. J Ethnopharmacol. 2005;100(3):333-8.
Andallu B, Varadacharyulu NC. Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats. Clin Chim Acta. 2003;338(1-2):3-10.
Sharma P, Tomar L, Bachwani M, Bansal V. Review on Neem (Azadirechta indica): thousand problem one solution. Int Res J Pharm. 2011;2(12):97-102.
Djenontin Tindo S, Amusant N, Dangou J, Wotto DV, Avlessi F, Dahouénon-Ahoussi E, et al. Screening of repellent, termiticidal and preventive activities on wood, of Azadirachta indica and Carapaprocera (Meliaceae) seeds oils. J Biol Sci. 2012;1(3):25-9.
Mondal D and Mondal T. A review on efficacy of Azadirachta indica A. Juss based biopesticides: an Indian perspective. Res J Recent Sci. 2012;1(3):94-9.
Shravan KD, Ramakrishna R, Santhosh KM, Kannappan N. In vivo antidiabetic evaluation of Neem leaf extract in alloxan induced rats. J App Pharm Sci. 2011;1(4):100-5.
Sithisarn P, Supabphol R, Gritsanapan W. Antioxidant activity of Siamese neem tree (VP1209). J Ethnopharmacol. 2005;99(1):109-12.
Aditi G, Bhandari BS, Rai N. Antimicrobial activity of medicinal plants Azadirachta indica A. Juss, Allium cepa L. and Aloe vera L. Int J PharmTech Res. 2011;3(2):1059-65.
Mbah AU, Udeinya IJ, Shu EN, Chijioke CP, Nubila T, Udeinya F, et al. Fractionated Neem leaf extract is safe and increases CD4 cell levels in HIV/AIDS patients. Am J Ther. 2007;14(4):369-74.
Charles V, Charles SX. The use and efficacy of Azadirachta indica ADR (‘Neem’) and Curcuma longa (‘Turmeric’) in scabies. A pilot study. Trop Geogr Med. 1992;44(1 2):178 81.
Bandyopadhyay U, Biswas K, Sengupta A, Moitra P, Dutta P, Sarkar D, et al. Clinical studies on the effect of Neem (Azadirachta indica) bark extract on gastric secretion and gastroduodenal ulcer. Life Sci. 2004;75(24):2867-78.
Isah AB, Ibrahim YK, Iwalewa EO. Evaluation of the antimalarial properties and standardization of tablets of Azadirachta indica (Meliaceae) in mice. Phytother Res. 2003;17(7):807-10.
Manal ME, Siddig IA, Fauziah O, Perichehr H, Ahmad BA, Adel SA. In vivo anti-tumor effect of Azadirachta indica in rat liver cancer. Res J Biol Sci. 2009;4(1):48-53.
Talwar GP, Raghuvanshi P, Misra R, Mukherjee S, Shah S. Plant immunomodulators for termination of unwanted pregnancy and for contraception and reproductive health. Immunol Cell Biol. 1997;75(2):190-2.
Vanka A, Tandon S, Rao SR, Udupa N, Ramkumar P. The effect of indigenous Neem Azadirachta indica mouth wash on Streptococcus mutans and Lactobacilli growth. Indian J Dent Res. 2001;12(3):133-44.
Chattopadhyay RR, Chattopadhyay RN, Maitra SK. Effects of neem on hepatic glycogen in rats. Indian J Pharmacol. 2000;25:174-5.
Walker HL, Mason AD Jr. A standard animal burn. J Trauma. 1968;8(6):1049-51.
Suzuki T, Hirayama T, Aihara K. Experimental studies of moderate temperature burns. Burns. 1991;17(6):443-51.
Niehaus WG Jr, Samuelsson B. Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem. 1968;6(1):126-30.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70-7.
Katsube T, Yamasaki M, Shiwaku K, Ishijima T, Matsumoto I, Abe K, et al. Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in diet-induced obese mice. J Sci Food Agric. 2010;90(14):2386-92.
Chen PN, Chu SC, Chiou HL, Kuo WH, Chiang CL, Hsieh YS. Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer Lett. 2006;235(2):248-59.
Kang TH, Hur JY, Kim HB, Ryu JH, Kim SY. Neuroprotective effects of the cyanidin-3-O-beta-d-glucopyranoside isolated from mulberry fruit against cerebral ischemia. Neurosci Lett. 2006;391(3):122-6.
Amarowicz R, Karamać M, Weidner S, Abe S, Shahidi F. Antioxidant activity of wheat caryopses and embryos extracts. J Food Lipids. 2002;9(3):201-10.
Arabshahi-Delouee S, Urooj A. Antioxidant properties of various solvent of mulberry (Morus indica L.) leaves. Food Chem. 2007;102(4):1233-40.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effect on superoxide radicals. Food Chem. 1999;64(4):555-9.
Bae SH, Suh HJ. Antioxidant activity of five different mulberry cultivars in Korea. LWT Food Sci Technol. 2007;40(6):955-62.
Raina R, Prawez S, Verma P, Pankaj N. Medicinal plants and their role in wound healing. Vet Scan. 2008;3(1):1-24.
