Published: 2017-01-19

Investigation of Convaren’s stimulating effect on immune organs of mice

Komila Porsokhonova, Rustam Agzamov


Background: Investigation of Convaren’s immunostimulating action on white mice used to prove its effectiveness as a new immunomodulatory drug that then may be used by humans for treatment of infectious, tumor, systemic, and other diseases followed by decrease of immune system function.

Methods: In this investigation were observed immunomodulating effects of Convaren on immune organs of mice (spleen, thymus, mesenteric lymph nodes, bone medulla). All effects were estimated due to the amount of antibody producing cells (APC) in their spleen, amount of APC calculated on whole organ and on 106 spleen cells, amount of nucleus containing spleen cells, common amount of nucleus containing cells in thymus, mesenteric lymph nodes, and bone medulla.

Results: Convaren in doses 50 and 100 mg/kg significantly increases amount of cells in thymus and bone medulla, but significant increase of cell amount in lymph nodes was observed just with dose 50 mg/kg. It shows that dose 50 mg/kg is optimum, with which the cell proliferation processes in central and peripheral immune organs are expressed maximally. Smaller dose of Convaren has a little better stimulating effect than the higher one.

Conclusions: New drug Convaren has a high immunostimulating activity in mice’s organisms. Due to its low toxicity, availability of raw materials and not hard production and transportation Convaren may be a cost-effective drug for the Asian region.


Convaren, Immunomodulator, Convolvulus arvensis L., Proliferation of immune cells

Full Text:



Khan MM. Immunopharmacology. New York: Springer Science+Business Media, LLC; 2008: 266-28.

Little M, editor. Recombinant Antibodies for Immunotherapy. Cambridge: Cambridge University Press; 2009: 434-419.

Murad W, Ahmad A, Gilani SA, Khan MA. Indigenous knowledge and folk use of medicinal plants by the tribal communities of Hazar Nao Forest, Malakand District, North Pakistan. J Med Plants Res. 2011;5(7):1072-1086.

Bowait ME. Albokhadaim IF, Homeida AM. Immunostimulant effects of bindweed (Convolvulus arvensis) extract in rabbits. Res J Pharmacol. 2010;4(2):51-4.

Mahmoudi M, Zamani Taghizadeh Rabe S, Zamani Taghizadeh Rabe S, Emami SA. A study to investigate the biological activity of proteoglycan mixture extract from Convolvulus arvensis. J Complement Integr Med. 2014;11:265-72.

Evans WC, Somanabhandhu A. Cuscohygrine: a constituent of the roots of some British Convolvulaceae. Phytochemistry. 1974;13:519.

Jerne NK, Nordin AA. Plaque formation in agar by single antibody-producing cells. Science. 1963;140(3565):405.

Cunningham AJ, Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968;14(4):599-600.

Thakral J, Borar S, Kalia JA. Antioxidant potential fractionation from methanol extract of aerial parts of Convolvulus arvensis Linn. (Convolvulaceae). Int J Pharm Sci Drug Res. 2010;2(3):219-23.

Kaur M, Kalia AN. Convolvulus arvensis – A useful weed. I.S.F. College of pharmaceutical sciences and research, Moga Punjab. Int J Pharm Pharm Sci 2012;4(1):38-41.

Elzaawely AA, Tawata S. Antioxident activity of phenolic rich fraction obtained from Convolvulus arvensis L. leaves grown in Egypt. Asian J Crop Sci. 2012;4(1):32-40.

Khakimova ZZ, Mustanov TB, Mirzokhidov KH. Investigation of anti-inflammatory activity of Convaren in local usage. Med J Uzbekistan. 2013;4(2):99-102.

Riordan NH, Menh X, Taylor P, Riordan HD. Anti-angiogenic, antitumor and immunostimulatory effects of a nontoxic plant extract (PMG). Allergy Research Group Focus Newsletter, March; 2001.

Calvino N. Anti-angiogenesis properties of a common weed, Convolvulus arevensis. J Chiropr Med. 2002;1:116.

Meng XL, Riordan NH, Casciari JJ, Zhu Y, Zhong J, González MJ, et al. Effects of a high molecular mass Convolvulus arvensis extract on tumor growth and angiogenesis. P R Health Sci J. 2002;21:323-8.

Khan MU, Ghori NH, Hayat MQ. Phytochemical analyses for antibacterial activity and therapeutic compounds of Convolvulus arvensis L., Collected from the salt range of Pakistan. Adv Life Sci. 2015;2(2):83-90.

Schultheiss PC, Knight AP, Traub-Dargatz JL, Todd FG, Stermitz FR. Toxicity of field bindweed (Convolvulus arvensis) to mice. Vet Hum Toxicol. 1995;37(5):452-4.