DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20151354

Evaluation of wound healing activity of ethanolic extract of Azadirachta Indica leaves on incision and excision wound models in Wister albino rats

H. N. Nagesh, P. L. Basavanna, M. S. Kishore

Abstract


Background: Wound healing is complex cellular and biochemical cascade that lead to restitution of integrity and function. Recently, the traditional use of plants for wound healing has received attention by the scientific community, as traditional medicine is a source of less expensive, comprehensive medical care, especially in developing countries. Azadirachta indica (Neem) is well-known in India, as one of the most versatile medicinal plants having a wide spectrum of biological activity. Hence, this study was undertaken to evaluate the wound healing activity of the ethanolic extract of A. indica leaf in the experimentally-induced wound in rats.

Methods: The healing effect produced by A. indica extract was assessed by the rate of wound contraction histopathology and skin breaking strength by using excision wound model and incision wound model in Wister albino rats. This was compared with control (soft white paraffin) and standard (1% w/w framycetin sulfate ointment). The results have been analyzed by calculating the mean values, standard deviation and compared by using student t-test.

Results: The ethanol extract of leaves of A. indica significantly promoted the wound healing activity in both excision and incision wound models.

Conclusion: The study revealed promising wound healing activity of ethanolic extract of A. indica and provides a scientific rationale for the traditional use in the management of wounds.


Keywords


Traditional medicine, Framycetin sulfate ointment, Soft white paraffin

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References


Clark RA. Wound repair: overview and general considerations. In: Clark RA, Henson PM, editors. The Molecular and Cellular Biology of Wound Repair. 2nd Edition. New York: Plenum Press; 1996: 473-88.

Akerele O. Nature’s medicinal bounty: don’t throw it away. World Health Forum. 1993;14(4):390-5.

Pirbalouti AG, Koohpayeh A, Karimi I. The wound healing activity of flower extracts of Punica granatum and Achillea kellalensis in Wistar rats. Acta Pol Pharm. 2010;67(1):107 10.

Biswas K, Chattopadhyay I, Banerjee RK, Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Curr Sci. 2002;82(11):1336-45.

Shafiuddin M, Khan A, Ali S. Wound healing activity of traditional herbal formulation. Int J Chem Sci. 2009;7(2):639 43.

Morton JJ, Malone MH. Evaluation of vulneray activity by an open wound procedure in rats. Arch Int Pharmacodyn Ther. 1972;196(1):117-26.

Singhal A, Gupta H, Bhati V. Wound healing activity of Argyreia nervosa leaves extract. Int J Appl Basic Med Res. 2011;1(1):36-9.

Ehrich HP, Hunk TK. Effect of cortisone and anabolic steroids on tensile strength of healing wound. Ann Surg. 1969;170:203-6.

Lee KH. Studies on the mechanism of action of salicylate. II. Retardation of wound healing by aspirin. J Pharm Sci. 1968;57(6):1042-3.

Barua CC, Talukdar A, Barua AG, Chakraborty A, Sharma RK, Bora RS. Evaluation of the wound healing activity of methanolic extract of Azadirachta indica (Neem) and Tinospora cordifolia (Guduchi) in rats. Pharmacol Online. 2010;1:70-7.

Lee G, Luna HT. Manual of Histological Staining Methods of the Armed Forces, Institute of Pathology. 3rd Edition. New York, Toronto, London, Sydney: American Registry of Pathology, The Blakiston Division; 1968: 68-9.

Pandey IP, Ahmed SF, Chhimwal S, Pandey S. Chemical composition and wound healing activity of volatile oil of leaves of Azadirachta indica A. juss. Adv Pure Appl Chem. 2012;1(3):62-6.

Amer H, Helmy WA, Taie HA. In vitro antitumor and antiviral activities of seeds and leaves neem (Azadirachta indica) extracts. Int J Acad Res. 2010;2(2):47-51.

Mandal R, Dhaliwal PK. Antifertility effect of Melia azedarach Linn. (Dharek) seed extract in female albino rats. Indian J Exp Biol. 2007;45(10):853-60.

Girish K, Shankara Bhat S. Neem - A green treasure. Electron J Biol. 2008;4(3):102-11.