Effect of Trigonella foenum (methi) on fasting blood glucose levels in alloxan induced diabetes in rabbits
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20164137Keywords:
Alloxan, Rabbits, Trigonella foenumAbstract
Background: Water-retaining fibers, especially the mucilaginous compounds, such as the gel fiber present in Fenugreek seeds, reduce the rate of glucose absorption and may also delay gastric emptying thereby preventing the rise in blood sugar levels following a meal. In addition to its hypoglycemic effects, the hyperlipidemic effect of Fenugreek fibers has also been documented. The objective of the study was to study the effect of Trigonella foenum (methi) on Fasting Blood Glucose levels in Alloxan induced diabetes in Rabbits.
Methods: Fenugreek seed extract was prepared. Study was carried out on alloxan induced diabetic rabbits. They were prepared for the study. Then they were grouped into 5 groups depending upon the drugs used for them. The drug solutions were prepared and administered to diabetic rabbits. The blood sugar levels were recorded before and after treatment for first 15 days and once in week for next three weeks. Daily fasting blood sugar readings were taken. The blood glucose was determined with standard procedures and methods.
Results: Alcohol extract of fenugreek seeds using soxhlet apparatus produced significant (P <0.001) reduction in the blood glucose levels in the alloxan induced diabetic rabbits in the 3 doses i.e. 0.5 gm/kg, 1 gm/kg and 1.5 gm/kg body weight after oral administration compared to the vehicle treated group (5 ml of 2% gum acacia) over the period of 5 weeks. The standard oral anti-diabetic drug (Metformin) in the dose of 62.5 mg/kg body weight also reduced the blood glucose level in the Alloxan induced diabetic rabbits over 5 weeks period of treatment compared to vehicle group. (5 ml of 2% gum acacia).
Conclusions: All the three doses of Trigonella foenum significantly (P<0.001) reduced blood glucose levels in Alloxan induced diabetic rabbits over a period of 5 weeks in comparison to vehicle (5 ml of 2% of gum acacia) orally.
References
Rother KI. Diabetes treatment – bridging the divide. N Engl J Med. 2007;356 (15):1499-501.
Chang AM, Halter JB. Aging and insulin secretion. Am J Physiol Endocrinol Metab. 2003;284(1):E7-12.
Cohen RV, Schiavon CA, Pinheiro JS et al. Duodenal-jejunal bypass for the treatment of type 2 diabetes in patients with body mass index of 22-34 kg/m2: a report of 2 cases". Surg Obes Relat Dis. 2007;3(2):195-7.
Garti N. Fenugreek Gum. The magic fiber for an improved glucose response and cholesterol reduction. NutraCos. 2002;1(3):5-10.
Asli Semiz. Various extraction procedures. African J Biotechnol. 2007;6(3):273-7.
Shani JA. Hypoglycemic effect of Trigonella Foenum Graceum in Alloxan Diabetic rats. Arch Interval Pharmacodyn. 1974;210(1):27-37.
Akthar AK, Athar MA, Yakub M. Effect of Momordica Charantia on blood sugar level of Normal and Alloxan rabbits. Planta Med. 1981;42:205-12.
Ojiako OA, Nwanjo HU. Effects of pliaglitazone on atherogenic risk predictor indices of alloxan induced diabetic rabbits. Biokemistri. 2005;17(2):179-84.
Babu V, Gangadevi T, Subram Oniam A. Antidiabetic activity of ethanol extract of cassia kleinii leaf in streptozotocin-induced diabetic rats and isolation of an active fraction and toxicity evaluation of the extract. Indian J Pharmacol. 2003;35(5):290-6.
Abdel-Barry JA, Abdel–Hassan IA, Al-Hakien MH. Hypoglycemic and antihyperglycemic effects of Trigonella foenum-graecum leaf in normal and alloxan induced diabetic rats. J Ethnopharmacol. 1997;58:149-55.
Vats V, Grover JK, Rathi SS. Evaluation of anti-hyperglycemic and hypoglycemic effect of Trigonella foenum-graecum Linn, Ocimum sanctum Linn and Pterocarpus marsupium Linn in normal and alloxanized diabetic rats. J Ethnopharmacol. 2002;79(1):95-100.
Khosla P, Gupta DD, Nagpal RK. Effect of Trigonella foenum graecum (Fenugreek) on blood glucose in normal and diabetic rats. Indian J Physiol Pharmacol. 1995;39(2):173-4.
Xue WL, Li XS, Zhang J et al. Effect of Trigonella foenum-graecum (fenugreek) extract on blood glucose, blood lipid and hemorheological properties in streptozotocin-induced diabetic rats. Asia Pac J Clin Nutr. 2007;16 Suppl 1:422-6.
Zia T, Hasnain SN, Hasan SK. Evaluation of the oral hypoglycaemic effect of Trigonella foenum-graecum L. (methi) in normal mice J Ethnopharmacol. 2001;75(2-3):191-5.
