Protective effect of Ipomoea aquatica against reserpine induced oxidative stress in brain using mice
Keywords:Ipomoea aquatica, Haloperidol, Lipid peroxidation, Biochemical analysis, Oxidative stress
Background: The aim of the present study was to investigate the protective potential of alcoholic extract of Ipomoea aquatica extract against haloperidol induced catatonia, reserpine induced hypokinesia and reserpine induced oxidative stress.
Methods: The mice were treated with reserpine for three days every other day. It was observed that Ipomoea aquatica MA extract significantly decreased haloperidol induced catatonia at 30, 60, 90 and 120 minutes respectively. The biochemical analysis of brain showed that the treatment with reserpine significantly enhanced peroxidation of lipid and lower levels of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GSH), which will be an index for oxidative stress process. Co-administration of extract at (200 mg/kg and 400 mg/kg) significantly lowered the lipid peroxidation and reversed the decrease in brain SOD, CAT and GSH levels.
Results: It was observed that the alcoholic extract of Ipomoea aquatica had a protective role against haloperidol induced catatonia, reserpine induced hypokinesia, and reserpine induced oxidative stress.
Conclusions: Ipomoea aquatica showed significant activity at the dose of (400 mg/kg).
Gupta YK, Gupta M, Kohli K. Neuroprotective role of melatoninin oxidative stress vulnerable brain. Indian J Physiol Pharmacol. 2003;47:373-86.
Wolff SP, Garner A, Dean RT. Free radicals lipids and protein degradation. Trends Biol Sci. 1986;11:27-31.
Kumar A, Kulkarni SK. Effect of BR-16A (Mentat), a polyherbal formulation on drug induced catalepsy in mice. Indian J Exper Biol. 2006;44:45-8.
Farde L, Nordstrom AL, Wiesel FA, Pauli S, Halldin C, Sedvall G. Positron emission tomographic analysis of central D1 and D2 dopamine receptor occupancy in patients treated with classical neuroleptics and clozapine: relation to extra-pyramidal side effects. Arch Gen Psychiatry. 1992;49:538-44.
Sanberg PR. Haloperidol-induced catalepsy is mediated by postsynaptic dopamine receptors. Nature 1980;284:472-3.
Sagara Y. Induction of reactive oxygen species in neurons by haloperidol. J Neurochem. 1998;71:1002-12.
Arjuman A, Nair V, Gopalkrishna HN, Nandini M. Evaluation of the antioxidant potential of NR-ANX-C (a polyherbalformulation) and its individual constituents in reversing haloperidol induced catalepsy in mice. Indian J Pharmacol. 2007;39:151-4.
Sajak AAB, Abas F, Ismail A, Alfi K. Effect of different drying treatments and solvent ratios on phytochemical constituents of Ipomoea aquatic and co-relation with α-glucosidase inhibitory activity. International J Food Properties. 2016;19(8):1859-64.
Sivaraman D, Panneerselvam P, Muralidharan P. In-vitro screening for acetylcholinesterase enzyme inhibition potential and antioxidant activity of extracts of ipomoea aquatica forsk: therapeutic lead for alzheimer’s disease. J Appl Pharmac Scien. 2015;5:12-6.
Mital N, Manvar D, Desai T. Exploration of possible mechanisms for anti-inflammatory activity of Ipomoea aquatica. International J Phytophar. 2015;5:110-8.
Khandelwal KR. Practical pharmacognosy. Nirali Prakashan, Pragati Books Pvt. Ltd; 2008;107-13.
Agrawal D, Chaudagar K, Appanna V, Mehta A. An antihistaminic activity of vitamin d in experimental models. World J Pharm Pharmace Scien. 2016;5:1489-97.
Sudhakar P, Nair V, Dorababu P, Gopalakrishna HN. Effect of ethanolic extract of leaves of ocimum sanctum on haloperidol induced catalepsy in albino mice. Indian J Pharmacol. 2007;39:87-9.
Eftimov M, Dobreva C, Velkova D, Kuzmanova S. Effect of Aronia melanocarpa fruit juice on reserpine-induced hypokinesia and oxydative stress in rats. Scripta Scien Pharma. 2014;1:19-34.
Sivaraman D, Muralidaran P. Nootropic effect of Ipomoea aquatica forsk in rat hippocampus. International J PharmTech Res. 2010;2:475-9.
Somani RS, Kasture VS, Kasture SB. Haloperidol inhibits (-) bicuculline-induced seizures and bicuculline potentiates haloperidol-induced catalepsy in mice. Indian J Pharmacol. 1999;31:434-6.
Shivakumar BR, RavindranathV. Oxidative stress and thiol modification induced by chronic administration of haloperidol. J Pharmacol Exp Ther. 1993;265:1137-41.
Elkashef AM, Wyatt RJ. Tardive dyskinesia: possible involvement of free radical and treatment with vitamin E. Schizophr Bull. 1999;25:731-40.
Ravindranath V, Reed DJ. Glutathione depletion and formation of glutathione protein mixed disulfide following expose of brain mitochondria to oxidative stress. Bioche Biophys Re Commun. 1990;169:150-8.
Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxy alkenal, malondialdehyde and related aldehydes. Free Radic Biol Med. 1991;11:81-128.
Banaclocha MN. Acetylcysteine elicited increase in complex I activity in synaptic mitochondria from aged mice: implications for treatment of Parkinson’s disease. Brain Res. 2000;850:173-1.