Evaluation of anti-aging effects of swimming exercise on inflammatory parameters in D-galactose-treated Wistar albino male rats
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20262858Keywords:
Aging, C-reactive protein, D-galactose, Inflammation, Interleukin-6, Swimming exerciseAbstract
Background: The present study was undertaken to investigate the anti-ageing effect of swimming exercise on inflammatory parameters in D-galactose-induced Wistar albino male rats.
Methods: Wistar albino rats were divided into four groups, namely, normal control, D-galactose-treated group (positive control), normal control with swimming exercise, and D-galactose-treated rats with swimming exercise. The experiment was conducted for 42 days. D-galactose was administered as a compound to induce ageing-like changes, and swimming exercise was used as a non-pharmacological treatment. Plasma was separated from blood samples on the 43rd day for estimation of inflammatory markers, interleukin-6 (IL-6) and c-reactive protein (CRP) taken from the tail vein.
Results: The plasma levels of IL-6 and CRP were significantly higher in the D-galactose-treated rats than in the normal control rats, suggesting an enhanced inflammatory response. The swimming exercise significantly reduced IL-6 and CRP levels in D-galactose-treated rats compared with the positive control group.
Conclusions: Swimming exercise was found to have a significant anti-inflammatory and anti-aging effect on Wistar albino male rats treated with D-galactose, as the plasma levels of IL-6 and CRP were reduced. Therefore, swimming exercise could be regarded as a useful non-pharmacological method to prevent inflammatory changes associated with aging.
References
López-Otín C, Blasco MA, Partridge L, Serrano M, Kroemer G. Hallmarks of aging: an expanding universe. Cell. 2023;186(2):243-78.
Franceschi C, Garagnani P, Parini P, Giuliani C, Santoro A. Inflammaging: a new immune-metabolic viewpoint for age-related diseases. Nat Rev Endocrinol. 2018;14(10):576-90.
Ferrucci L, Fabbri E. Inflammageing: chronic inflammation in ageing, cardiovascular disease, and frailty. Nat Rev Cardiol. 2018;15(9):505-22.
Ershler WB, Keller ET. Age-associated increased interleukin-6 gene expression, late-life diseases, and frailty. Annu Rev Med. 2000;51:245-70.
Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest. 2003;111(12):1805-12.
Azman KF, Zakaria R. D-galactose-induced accelerated aging model: an overview. Biogerontology. 2019;20(6):763-82.
Sadigh-Eteghad S, Majdi A, McCann SK, Mahmoudi J, Vafaee MS, Macleod MR. D-galactose-induced brain ageing model: a systematic review and meta-analysis. PLoS One. 2017;12(8):e0184122.
Radak Z, Suzuki K, Higuchi M, Balogh L, Boldogh I, Koltai E. Physical exercise, reactive oxygen species and neuroprotection. Free Rad Biol Med. 2016;98:187-96.
Huang CC, Chiang WD, Huang WC, Huang CY, Hsu MC, Lin WT. Hepatoprotective effects of swimming exercise against D-galactose-induced senescence rat model. Evid Based Complement Alternat Med. 2013;2013:275431.
Parameshwaran K, Irwin MH, Steliou K, Pinkert CA. D-galactose effectiveness in modeling aging. Rejuv Res. 2010;13(6):729-35.
Gobatto CA, de Mello MA, Sibuya CY, de Azevedo JR, dos Santos LA, Kokubun E. Maximal lactate steady state in rats submitted to swimming exercise. Comp Biochem Physiol Mol Integr Physiol. 2001;130(1):21-7.
Engvall E, Perlmann P. Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972;109(1):129-35.
Bruunsgaard H, Pedersen BK. Age-related inflammatory cytokines and disease. Immunol Allerg Clin North Am. 2003;23(1):15-39.
Petersen AMW, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol. 2005;98(4):1154-62.
Pedersen BK, Febbraio MA. Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiol Rev. 2008;88(4):1379-406.
Gleeson M, Bishop NC, Stensel DJ, Lindley MR, Mastana SS, Nimmo MA. The anti-inflammatory effects of exercise: mechanisms and implications for prevention and treatment of disease. Nat Rev Immunol. 2011;11(9):607-15.
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