To evaluate and compare antidepressant activity of Centella asiatica in mice by using forced swimming test


  • Padmavathi Golla ABB GISPL, Bangalore, Kamataka, India
  • Hemapriya Tirupathi SVIMS-Sri Padmavathi medical college for women Tirupathi, Andhra Pradesh, India



Centella asiatica, Mice, Immobility time, Anti-depressant like effect


Background: Centella asiatica is a great multipurpose miracle herb used in Oriental medicine that has several therapeutic (such as sedative and hypnotic) also heals depression, grief, nervous stress and tension. In the present study we evaluated antidepressant-like effect of Centella asiatica using forced swimming test (FST).The forced swim test is a commonly used stressor test, where mice are forced to swim in specially constructed tanks for a particular period where there is behavioural activation characterized by vigorous swimming and diving to search for alternate routes to escape. Animal health including human has been shown to affected by the stressful events of the life inducing situation which alters cognition, learning memory and emotional responses, causing mental disorders like depression and anxiety and stress in mice.

Methods: The experiment was carried out with 24 healthy albino mice of either sex weighing about 25-30 gm. These were acclimatized to their environment for one week prior to experientation. Two doses of aqueous extract (10 and 20 mg/kg) was injected intraperitoneally. After 30 min of injection, immobility and swimming times were measured and compared with control (negative control) and imipramine (positive control).

Results: In the present study Centella asiatica in the doses of 10 mg and 20 mg/kg significantly decreased the immobility time compared to the control than standard, thereby proving that it has anti-depresssant activity. The dose of 20 mg/kg body wt. showed anti-depressant activity almost similar to the standard drug imipramine (10 mg/kg).

Conclusions: Due to lack of certain records, it is envisaged that the change of medicine both discontinuation as well as addition was done because of blood glucose control, cost factor (in case of pioglitazone) as well as patient’s compliance.


Gopalreddygari V, Siva P, Sahitya C. Evaluation of anticonvulsant activity of Centella asiatica (gotu kola) in pentylenenetetrazole induced seizure with respect to cholinergic transmission. Epilepsy and behavior. 2000;17(3):332-5.

Nadkarni KM, Nadkarni AK. Indian material medica, III edition. Bombay: Popular Prakashan; 2005:662-5.

Brinkhaus B, Lindner M, Schuppan D, Hahn EG. Chemical, pharmacological and clinical profile of east asian madicinal plant Centella asiatica. Phytomedicine. 2000;7:427-48.

Maquart FX, Chastang F, Simeon A, BirembautP, Gillery P. Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds. Eur J Dermatol. 1999;9(4):289-96.

Nalini K, Aroor AR, Karanth KS, Rao A. Effect of Centella asiatica fresh leaf aqueous extract on learning and memory and biogenic amine turnover in albino rats. Fitoterapia. 1992;63:232-7.

Abdulla MA, Bayaty FH, Younis LT. Anti-ulcer activity of Centella asiatica leaf extract against ethanol-induced gastric mucosal injury in rats. Journal of Medicinal Plants Research. 2010;4(13):1253-9.

The Ayurvedic Pharmacopoeia of India, Ministry of health and family welfare. 2000;4:69-71.

Visweswari G, Prasad KS, Lokanatha V, Rajendra W. The antiepileptic effect of Centella asiatica on the activities of Na + /K +, Mg 2+ and Ca 2+ -ATPases in rat brain during pentylenetetrazol-induced epilepsy. Indian J of pharmacol. 2010;42(2):82-6.

Lee MK, Kim SR, Sung SH, Lim D, Kim H. Asiatic acid derivatives protect cultured cortical neurons from glutamate-induced excitotoxicity. Res Commun Mol Pathol Pharmacol. 2000;108(1):75-86.

Gupta YK, Veerendra Kumar MH and SrivastavaAK. Effect of Centella asiatica on pentylenetetrazole-induced kindling,cognition and oxidative stress in rats. Pharmacol Biochem Behav. 2003;74(3):579-85.

Gupta SK. Drug screening method. 1st edition. New delhi: Jaypee; 2004:92-5.

Chen Y, Han T, Qin L, Rui Y, Zheng H. Effect of total triterpenes from Centella asiatica on the depression behavior and concentration of amino acid in forced swimming mice. Pharmacol, biochem and behavior. 2003;26(12):870-3.

Thamarai SP, Senthil KM. Antidepressnt activity of etanolic extract of leaves Centella asiatica. Asian J Res Pharm Sci. 2012;2(2):76-9.




How to Cite

Golla, P., & Tirupathi, H. (2017). To evaluate and compare antidepressant activity of Centella asiatica in mice by using forced swimming test. International Journal of Basic & Clinical Pharmacology, 5(5), 2017–2020.



Original Research Articles