DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20163217

To evaluate and compare antidepressant activity of Rosa damascena in mice by using forced swimming test

Hemapriya Tirupathi, Padmavathi Golla

Abstract


Background: Rosa damascene mill L (R. damascena) is an ornamental plant that has several therapeutic (such as sedative and hypnotic) effects. It also heals depression, grief, nervous stress and tension. In the present study we evaluated antidepressant-like effect of R. damascena using forced swimming test (FST). The forced swim test is a commonly used stressor test, where mice are forced to swim in specially constructed tanks for a particular period and looked for behavioural activation characterized by vigorous swimming and diving to search for alternate routes to escape. Animal health including human has been shown to be affected by the stressful events of the life inducing situation which alters cognition, learning memory and emotional responses, causing mental disorders like depression and anxiety and stress in mice.

Methods: The experiment was carried out with 24 healthy albino mice of either sex weighing about 25-30 gm. These were acclimatized to their environment for one week prior to experimentation. Two doses of aqueous extract (20 and 40 mg/kg) were injected intraperitoneally. After 30 min of injection, immobility and swimming times were measured and compared with control (negative control) and imipramine (positive control).

Results: In the present study R. damascena in the doses of 20 mg and 40 mg/kg significantly decreased the immobility time compared to the control than standard, thereby proving that it has antidepresssant activity. The dose of 40 mg/kg body weight showed anti-depressant activity almost similar to the standard dug imipramine (10 mg/kg).

Conclusions: Antidepressant activity of R. damascena was studied by forced swimming test in mice in the doses of 20 mg/kg and 40 mg/kg .This test shows that R. damascena significantly decreased the immobility time in mice. The results suggest that R. damascena has dose dependent antidepressant activity comparable with imipramine.


Keywords


Rosa damascena, Mice, Immobility time, Antidepressant like effect

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