In vitro evaluation of antifungal property of ethanolic extract of Syzygium aqueum fruit in leucorrhoea using zone of inhibition

Deepika Lakshmi P.; Sridevi S. A.; Rishaba Varma P.

doi:10.18203/2319-2003.ijbcp20254150

Authors

Deepika Lakshmi P. Department of Pharmacology, ACS Medical College and Hospital, Chennai, Tamil Nadu, India
Sridevi S. A. Department of Pharmacology, ACS Medical College and Hospital, Chennai, Tamil Nadu, India
Rishaba Varma P. Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20254150

Keywords:

Antifungal, Ethanolic extract, Leucorrhoea, Syzygium aqueum, Zone of inhibition

Abstract

Background: Leucorrhoea, characterized by abnormal vaginal discharge, is commonly associated with Candida albicans infection. Conventional antifungal agents face resistance, creating the need for plant-based alternatives. Syzygium aqueum (water apple) is known to possess antimicrobial activity.

Methods: Ethanolic extract of Syzygium aqueum fruit was prepared by cold maceration. Antifungal activity was evaluated against Candida albicans using agar well diffusion on potato dextrose agar medium. Extract concentrations (10, 5, 2.5, 1.25 mg/ml) were tested. DMSO served as negative control, and ketoconazole (50 mg/ml) as positive control. Zones of inhibition were measured after incubation at 37°C for 24 hours.

Results: Extract exhibited concentration-dependent antifungal activity with maximum inhibition zone of 20 mm at 10 mg/ml, while ketoconazole showed 31 mm inhibition.

Conclusions: Syzygium aqueum demonstrated moderate antifungal efficacy against Candida albicans, supporting its potential as a natural antifungal option in leucorrhoea management.

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