Evaluation of the effects of Aegle marmelos and Punica granatum in an experimental model of gastrointestinal barrier dysfunction

Harshveer Singh M. Malhi; Sandhya K. Kamat; Akash R. Deogharkar; Nirmala N. Rege

doi:10.18203/2319-2003.ijbcp20163200

Authors

Harshveer Singh M. Malhi Department of Pharmacology and Therapeutics, Seth G.S. Medical College and KEM Hospital, Mumbai, India
Sandhya K. Kamat Department of Pharmacology and Therapeutics, Seth G.S. Medical College and KEM Hospital, Mumbai, India
Akash R. Deogharkar Department of Pharmacology and Therapeutics, Seth G.S. Medical College and KEM Hospital, Mumbai, India
Nirmala N. Rege Department of Pharmacology and Therapeutics, Seth G.S. Medical College and KEM Hospital, Mumbai, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20163200

Keywords:

Bacterial translocation, Obstructive jaundice, Disaccharidase, Sucrose, Villi

Abstract

Background: The study was conducted to evaluate the effects of Aegle marmelos and Punica granatum in an experimental model of gastrointestinal barrier dysfunction induced by common bile duct ligation.

Methods: Institutional animal ethics committee approval was obtained. Forty two Wistar rats (either sex, 150-250 gms) divided into seven groups (n=six/group), were subjected to sham operation (group 1) or bile duct ligation (groups 2-7) and treated with distilled water (groups 1 and 2); 0.75mg/kg glutamine (group 3); 0.27 g/kg and 0.54 g/kg of A. marmelos (groups 4 and 5); 3.6 g/kg and 7.2 g/kg P. granatum (groups 6 and 7) orally once daily for 10 days. On Day 11, animals were sacrificed and samples of the jejunum, ileum and mesenteric lymph nodes were obtained to study jejunal and ileal villous morphology, villous heights, jejunal mucosal sucrase enzyme activity and bacterial translocation to mesenteric lymph nodes.

Results: Glutamine prevented blunting of the intestinal villi, bacterial translocation and a fall in the sucrase enzyme activity. Both the plant drugs prevented blunting of the villi (except low dose A. marmelos for ileal villi) and a fall in the villous heights (except low dose P. granatum for jejunal villi), decreased the bacterial translocation (except low dose A. marmelos), and prevented a fall in the sucrase enzyme activity when compared to the disease control. The high doses of both A. marmelos and P. granatum were comparable to glutamine for all the variables tested.

Conclusions: Both A. marmelos and P. granatum maintained the gastrointestinal barrier function in this model.

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References

Blikslager AT, Moeser AJ, Gookin JL, Jones SL, Odle J. Restoration of barrier function in injured intestinal mucosa. Physio Rev. 2007;87(2):545-64.

Lopetuso LR, Scaldaferri F, Bruno G, Petito V, Franceschi F, Gasbarrini A. The therapeutic management of gut barrier leaking: the emerging role for mucosal barrier protectors. Eur Rev Med Pharmacol Sci. 2015;19(6):1068-76.

Farhadi A, Banan A, Fields J, Keshavarzian A. Intestinal barrier: an interface between health and disease. J Gastroenterol Hepatol. 2003;18(5):479-97.

Bischoff SC, Barbara G, Buurman W, Ockhuizen T, Schulzke JD, Serino M, et al. Intestinal permeability a new target for disease prevention and therapy. BMC gastroenterology. 2014;14(1):1.

Scaldaferri F, Pizzoferrato M, Gerardi V, Lopetuso L, Gasbarrini A. The gut barrier: new acquisitions and therapeutic approaches. J Clin Gastroenterol. 2012;46:12-7.

Gogte VM. Medicinal Plants. In: Ayurvedic Pharmacology and Therapeutic Uses of Medicinal Plants (Dravyagunavignyan). 1st English edition. Mumbai: Bharatiya Vidya Bhavan; 2000:392-442.

Brijesh S, Daswani P, Tetali P, Antia N, Birdi T. Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: validating its traditional usage. BMC Complement Altern Med. 2009;9:47.

Qnais EY, Elokda AS, Abu Ghalyun YY, Abdulla FA. Antidiarrheal activity of the aqueous extract of Punica granatum (Pomegranate) Peels. Pharmaceutical Bio. 2007;45(9):715-20.

Wang B, Wu G, Zhou Z, Dai Z, Sun Y, Ji Y, et al. Glutamine and intestinal barrier function. Amino Acids. 2015;47(10):2143-54.

Xue H, Sawyer MB, Field CJ, Dieleman LA, Murray D, Baracos VE. Bolus oral glutamine protects rats against CPT-11-induced diarrhea and differentially activates cytoprotective mechanisms in host intestine but not tumor. J Nutr. 2008;138(4):740-6.

Lee E. The effect of obstructive jaundice on the migration of reticulo-endothelial cells and fibroblasts into early experimental granulomata. Br J Surg. 1972;59(11):875-7.

Lee FD, Toner PG. The normal intestinal mucosa. In. Lee FD, Toner PG Eds. Biopsy Pathology of small intestine. London; Champal and Hall: 1977; 19-31.

Dahlqvist A. Assay of intestinal disaccharidases. Anal Biochem. 1968;22(1):99-107.

Perrone EE, Liu L, Turner DJ, Strauch ED. Bile salts increase epithelial cell proliferation through HuR-induced c-Myc expression. J Surg Res. 2012;178(1):155-64.

Gencay C, Kilicoglu SS, Kismet K, Kilicoglu B, Erel S, Muratoglu S, et al. Effect of honey on bacterial translocation and intestinal morphology in obstructive jaundice. World J Gastroenterol. 2008;14(21):3410-5.

Geyik MF, Aldemir M, Hosoglu S, Ayaz C, Satilmis S, Buyukbayram H, et al. The effects of Saccharomyces boulardii on bacterial translocation in rats with obstructive jaundice. Ann R Coll Surg Engl. 2006;88(2):176-80.

Assimakopoulos SF, Scopa CD, Vagianos CE. Pathophysiology of increased intestinal permeability in obstructive jaundice. World J Gastroenterol. 2007;13(48):6458-64.

Das SK, Roy C. The protective role of Aegle marmelos on aspirin-induced gastro-duodenal ulceration in albino rat model: a possible involvement of antioxidants. Saudi J Gastroenterol. 2012;18(3):188.

Shiban MS, Al MM, Al NS. Antioxidant activity of pomegranate (Punica granatum L.) fruit peels. Food Nutrition Sci. 2012;3(7):991.

Murthy K, Reddy V, Veigas J, Murthy U. Study on wound healng activity of Punica granatum peel. J Med Food. 2004;7(2):256-9.

Berg RD. Bacterial translocation from the gastrointestinal tract. Trends Microbiol. 1995;3(4):149-54.

Pai V, Chanu TR, Chakraborty R, Raju B, Lobo R, Ballal M. Evaluation of the antimicrobial activity of Punica granatum peel against the enteric pathogens: an in vitro study. Asian J Plant Sci Res. 2011;1(2):57-62.

Betanzos CG, Montes PY, Fabela HE, Belefant MH, Cancino DJC. Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis. Food Nutrition Res. 2015;59:27620.

Prasad D, Kunnaiah R. Punica granatum: A review on its potential role in treating periodontal disease. J Indian Society Periodontology. 2014;18(4):428.

Varga J, Toth Jr S, Toth S, Tomeckova V, Gregova K, Vesela J. The relationship between morphology and disaccharidase activity in ischemia-reperfusion injured intestine. Acta Biochim Pol. 2012;59(4):631-8.

Mohammadi S, Sagar S. Host Immunity and pathogen strain contribute to intestinal disaccharidase impairment following gut impairment. J Immunol. 2011;187(7):3769-75.