Analgesic activity of ethanolic extract of leaves of Nyctanthes arbor-tristis linn on experimental animal models


  • Pallavi Bordoloi Department of Pharmacology, Assam Medical College, Dibrugarh, Assam, India
  • Tarali Devi Department of Pharmacology, Assam Medical College, Dibrugarh, Assam, India
  • Mangala Lahkar Department of Pharmacology, Guwahati Medical College, Guwahati, Assam, India



Analgesic, Flavonoids, Nyctanthes arbortristis


Background: The present study has been undertaken to evaluate the pharmacological efficacy of ethanolic extract of leaves of Nyctanthes arbor-tristis (EENA) LINN as an analgesic agent in comparison with standard non-steroidal anti-inflammatory agents.

Methods: The extract was prepared by soxhlet method and acute toxicity test was performed as per OECD guidelines. The peripheral analgesic activity was assessed using acetic acid induced writhing method. The central analgesic activity was assessed using tail-flick method.

Results: It has been shown that EENA 400mg/kg and aspirin 100mg/kg significantly increased the pain threshold as assessed by increase in the latency period or basal reaction time. EENA (200mg, 400 mg/kg) and aspirin 100 mg/kg also significantly reduced acetic acid induced writhing response showing peripheral analgesic activity.

Conclusions: Since prostaglandins are involved in the pain perception and are inhibited by flavonoids, it could be suggested that the analgesic effect of Nyctanthes arbortristis might be due to its inhibitory action on PG biosynthesis.


Mersky H. Pain terms - a list with definitions and notes on usage. Pain. 1979;6:219-52.

Fields HL, Martin JB. Pain: Pathophysiology and Management. In: Kasper DL, Braunwald E, Fauci AS, Hauser SL, Longo DL, Jameson JL(eds). Harrison’s Principles of internal medicine, 17th ed. New York, McGraw Hill, 2008:81-86.

Sandhar HK, Kaur M, Kumar B, Prasher S. An update on Nyctanthes arbour-tristis Linn. Internationale Pharmaceutica Sciencea. 2011;1(1):77-86.

Kirtikar K R, Basu BD, Singh B, Singh MP. Indian medicinal plants. 1975;1(2):2392-93.

Nairn JG. Solutions, emulsions, suspension and extracts. In: Gennaro A, Marderosian AD, Hanson GR, Medwick T, Popovich NG, Schnaare RL. The science and practice of pharmacy, 20th edition. Philadelphia, Lippincott Williams and Wilkins; 2000: 721-752.

OECD Guidelines for testing of Chemicals (Internal), France: OECD publishing; 2006, Section 4, Health effects: Test No. 425: Acute oral toxicity: Up and down procedure (Adopted 2006, March 23).

Saxena RS, Gupta B, Saxena KK, Singh R, Prasad DN. Study of anti-inflammatory activity of the leaves of Nyctanthes arbortristis Linn.-An Indian medicinal plant. J Ethnopharmacol. 1984;11:319-30.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. International Pharmaceutica Sciencia. 2011;1(1):98-106.

D’Armour FE, Smith DL. A method for determining loss of pain sensation. J Pharmacol Exp Ther. 1941;72:74-9.

Ghosh MN. Toxicity studies. In: Fundamentals of Experimental Pharmacology 4th ed. Kolkata, India, Ghosh and others, 2008: 176-183.

Rao KV. Multiple comparision test procedures. In: Biostatistics. Rao KV, Balakrishnan N(eds). Biostatistics, 1st ed. New Delhi, India, Jaypee Brothers Medical Publishers, 1999:273-278.

Bhukya B, Anreddy RNR, William CM, Gottumukkda KM. Analgesic and anti-inflammatory activities of leaf extract of Kydea calycina Roxb. Bangladesh J Pharmacol. 2009;4:101-4.

Saxena RS, Gupta B, Saxena KK, Srivastava VK, and Prasad DN: Analgesic, antipyretic and ulcerogenic activity of Nyctanthes arbortristis leaf extract, Journal of Ethnopharmacol: 193-200, 1987.

Zaheer S.H, Prasad B., Chopra R.N., Santapan H and Krishnan M.S: The Wealth of India, Vol. VII, Publications and Information Directorate, C.S.I.R., New Delhi. 1966. pp. 69-70.

Ramaswamy S, Pillai NP, Gopal krishnan V, Parmar NS, Ghosh MN. Analgesic effect O-(β-hydroxy ethyl) rutoside in mice. Indian J Exp Biol. 1985;23:219-20.

Alcazar MJ, Jimenez MI, Flavonoids as anti-inflammatory agents. Filoterapia. 1988;59:25-38.




How to Cite

Bordoloi, P., Devi, T., & Lahkar, M. (2016). Analgesic activity of ethanolic extract of leaves of Nyctanthes arbor-tristis linn on experimental animal models. International Journal of Basic & Clinical Pharmacology, 5(6), 2598–2601.



Original Research Articles