High levels of cardiac and oxidative stress biomarkers ameliorated following treatment with aspirin, vitamin C, and Justicia secunda hydromethanol leaf extract on epinephrine induced-myocardial infarction in male Wistar rats

Samuel K. Mobisson; Iheanyichukwu  Wopara; Justin B. Monye; Uchechukwu L. Iyanyi; Desmond Izunwanne; Agona O. Obemebe

doi:10.18203/2319-2003.ijbcp20252561

Authors

Samuel K. Mobisson Department of Human Physiology, Faculty of Basic Medical Sciences, Federal University Wukari, Taraba State, Nigeria
Iheanyichukwu Wopara Department of Medical Biochemistry, Faculty of Sciences, Rivers State University, Nkpolu, Nigeria
Justin B. Monye Department of Human Physiology, Faculty of Basic Medical Sciences, Madonna University, Nigeria
Uchechukwu L. Iyanyi Department of Pharmacology, Faculty of Pharmacy, David Umahi Federal University of Health Sciences, Uburu, Ebonyi State, Nigeria
Desmond Izunwanne Department of Human Physiology, Faculty of Basic Medical Sciences, Imo State University, Owerri, Nigeria
Agona O. Obemebe Department of Human Physiology, Faculty of Basic Medical Sciences, University of Calabar, Cross River State, Nigeria

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20252561

Keywords:

Epinephrine, Justicia secunda, Lactate dehydrogenase, Myocardial infarction, Troponin T

Abstract

Background: Myocardial infarction is an increasing cardiac disorder globally. This study investigated the ameliorative potentials of Justicia secunda hydromethanol leaf extract on epinephrine-induced myocardial infarction in male Wistar rats.

Methods: Twenty-four rats weighing (70-170 gm) were assigned into 6 groups (n=4). Group A was control, group B received epinephrine (0.08 mg/kg) intraperitoneally for 2 days; groups C-F received epinephrine followed by oral treatments: Aspirin (2 mg/kg), Justicia secunda (1 mg/kg), Justicia secunda (2 mg/kg) and vitamin C (2 mg/kg) respectively for 14 days.

Results: There was a significant elevation of lactate dehydrogenase and troponin T and disruption in electrolyte balance, indicative of myocardial injury. Treatment with Justicia secunda (2 mg/kg), aspirin, and vitamin C significantly reduced LDH and troponin T levels (p<0.01), improved antioxidant status (increased SOD, CAT, GSH; decreased MDA), and slightly normalized electrolytes. Justicia secunda (2 mg/kg), exhibited improvement in high-density-lipoproteins and reduction in low-density-lipoprotein levels.

Conclusions: Treatment with Justicia secunda (particularly at 2 mg/kg), aspirin, and vitamin C ameliorated the epinephrine-induced myocardial infarction. Hence, if these results apply to humans, using Justicia secunda in management of myocardial infarction and oxidative stress should be encouraged as a potential natural adjunct.

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