Formulation and evaluation of herbal gel containing ethanolic extract of Momordica charantia against socransky’s periodontal pathogens of the oral cavity-an in vitro study

Authors

  • Shruti Karvekar Department of Periodontics, KAHER’s KLE V.K Institute of Dental Sciences, Belgaum, Karnataka, India India
  • Jasleen Thakker Department of Periodontics, KAHER’s KLE V.K Institute of Dental Sciences, Belgaum, Karnataka, India India
  • Vilas Pattar Department of Periodontics, KAHER’s KLE V.K Institute of Dental Sciences, Belgaum, Karnataka, India India
  • Vinita Krishna Department of Periodontics, KAHER’s KLE V.K Institute of Dental Sciences, Belgaum, Karnataka, India India
  • Ritashna Kaur Dhaleria Department of Periodontics, KAHER’s KLE V.K Institute of Dental Sciences, Belgaum, Karnataka, India India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20243038

Keywords:

Antimicrobial activity, Herbal gel, Momordica charantia, Periodontal pathogens

Abstract

Background: Periodontitis is a multifaceted disease initiated by periodontal pathogens which when left untreated results in inflammation of the supporting tissues of the teeth, resorption of alveolar bone, loss of the periodontal ligament attachment, periodontal pocket formation and/or recession of the gingiva. Hence, the current study aimed at evaluating the efficacy of Momordica charantia leaves extract on Socransky’s periodontal pathogens and formulating a herbal gel for subgingival local drug delivery for the potential treatment of moderate periodontitis.

Methods: M. charantia leaves were authenticated from a recognized taxonomist. They were coarsely powdered following which an ethanol-based extract preparation was done. The extract obtained was then assessed for minimum inhibitory concentration, minimum bactericidal concentration followed by gel formulation which was then subjected to time kill assay and zone of inhibition on periodontal pathogens, the efficacy of which was comparatively evaluated against 0.12% chlorhexidine (CHX) gluconate. Kruskal-Wallis Test was employed with the statistical significance set at p≤0.05.

Results: The concentration of M. charantia ethanolic extract against periodontal pathogens was determined to be 1 gm/100 ml and a statistically significant difference was found in the effectiveness between the gel so formulated and CHX.

Conclusion: The antibacterial activity was evident in the ethanolic extract of M. charantia which when used to formulate a herbal gel against anaerobic periodontal pathogens demonstrated efficacy comparable to that of CHX.

References

Dubey P, Mittal N. Periodontal diseases- a brief review. Int J Oral Health Dent. 2020;6(3):177-87

Teles RP, Haffajee AD, Socransky SS. Microbiological goals of periodontal therapy. Periodontol. 2006;42:180-218.

Lou Z, Wang H, Zhu S, Ma C, Wang Z. Antibacterial activity and mechanism of action of chlorogenic acid. J Food Sci. 2011;76:2-9.

Islam T, Hayat BA, Datta S, Akter S. Antimicrobial activity of medicinal plants on Streptococcus mutans, a causing agent of dental caries. Int J Eng Res Technol. 2012;1:1-6.

Leelaprakash G, Rose J, Gowtham BM, Prasad S. A in vitro antimicrobial and antioxidant activity of Momordica charantia leaves. Pharmacophore. 2011;2:244-52.

Kemparaj U, Chavan S, Pavani T. Effect of bitter gourd against S. mutans and L. acidophilus-An in vitro study. Int Ayurvedic Med J. 2014;2:374-9.

Mada SB, Garba A, Mohammed HA, Muhammad A, Olagunju A, Muhammad AB. Antimicrobial activity and phytochemical screening of aqueous and ethanol extracts of Momordica charantia L. leaves. J Med Plant Res. 2013;7(10):579-86.

Basch E, Gabardi S, Ulbricht C. Bitter melon (Momordica charantia): a review of efficacy and safety. American Journal of Health-System Pharmacy. 2003;60(4):356-9.

Costa JG, Nascimento EM, Campos AR, Rodrigues FF. Antibacterial activity of M. charantia (Curcubitaceae) extracts and fractions. J Basic and Clinical Pharm. 2010;2(1):45.

Kumar DS, Sharathnath KV, Yogeswaran P, Harani A, Sudhakar K, Sudha P, Banji D. A medicinal potency of Momordica charantia. Int J Pharm Sci Rev Res. 2010;1(2):95-100.

Grover JK, Yadav SP. Pharmacological actions and potential uses of Momordica charantia: a review. Journal of ethnopharmacology. 2004;93(1):123-32.

Brennan KT, Kirby JE. Antimicrobial synergy testing by the inkjet printer-assisted automated checkerboard array and the manual time-kill method. J Vis Exp. 2019;18:58636.

Rubab S, Bahadur S, Hanif U, Durrani AI, Sadiqa A, Shafique S, et al. Phytochemical and antimicrobial investigation of methanolic extract/fraction of Ocimum basilicum L. Biocatal Agric Biotechnol 2021;31:101894.

Joshi RK. Chemical composition and antimicrobial activity of the essential oil of Ocimum basilicum L. (Sweet Basil) from Western Ghats of North West Karnataka, India. Anc Sci Life. 2014;33:151-6.

Kaya I, Yigit N, Benli M. Antimicrobial activity of various extracts of Ocimum basilicum L. And observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Afr J Tradit Complement Altern Med. 2008;5:363-9.

Palombo EA. Traditional medicinal plant extracts and natural products with activity against oral Bacteria: Potential application in the prevention and treatment of oral diseases. Evid Based Complement Alternat Med. 2011;2011:680354.

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Published

2024-10-23

How to Cite

Karvekar, S., Thakker, J., Pattar, V., Krishna, V., & Dhaleria, R. K. (2024). Formulation and evaluation of herbal gel containing ethanolic extract of Momordica charantia against socransky’s periodontal pathogens of the oral cavity-an in vitro study. International Journal of Basic & Clinical Pharmacology, 13(6), 877–883. https://doi.org/10.18203/2319-2003.ijbcp20243038

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Original Research Articles