Renal biomarkers of Rosemarinus officinalis L. on gentamicine induced acute kidney injury in adult male albino rats (Rattus norvegicus)

Authors

  • Kennedy W. Waswa Department of Human Anatomy, School of Medicine, Maseno University, Kenya
  • Scolastica Kori Department of Microbiology, School of Medicine, Maseno University, Kenya

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20242414

Keywords:

Gentamicin, Acute kidney injury, Biomarkers, Antioxidant, Rosmarinus officinalis, Oxidation

Abstract

Background: Gentamicin (GN) drug is a common choice for treating severe gram-negative bacteria infections especially in resource-limited settings where cost effective options are considered. However, its potential effect of causing acute kidney injury (AKI) has limited its wide use in clinical applications. R. officinalis (rosemary) is a traditional herb rich in antioxidants and anti-inflammatory properties. Conversely, renal biomarkers effect of Rosmarinus Officinalis (RO) on GN has not yet been elucidated. The main objective was to investigate the renal biomarkers and protective effect of R. officinalis against Gentamicin induced acute Kidney injury in adult Albino rats.

Methods: 25 adult male Albino rats were equally divided into five groups (5 Albino rats per group). Control group received rat pellets plus water ad libitum, GN (100 mg/kg/bwt/i.p), low dose RO (RO 100 mg/kg/bwt/po+GN), Medium RO(150 mg/kg/bwt/po+GN), and high dose RO (200 mg/kg/bwt/po+GN) groups. GN was administered intraperitoneal, and RO orally for seven consecutive days.  After completion of 24hours of the last drug administration, they were humanely sacrificed, serum biochemical analysis of renal biomarkers (creatinine and urea) was determined.

Results: Gentamicin was able to induced acute kidney injury by increasing levels of creatinine and urea. However, with concurrent administration of high dose of RO, Serum creatinine and urea remained within the normal ranges.

Conclusions: These findings showed that, Concurrent administration of a high dose of RO 200 mg/kg/bwt with Gentamicin protects kidneys against Gentamicin induced injuries.

Metrics

Metrics Loading ...

References

Hoste EA. Epidemiology of acute kidney injury in critically ill patients: the multinational AKI-EPI study. J Intensive Care Med. 2015;41:1411-23.

Mehta RL. International society of nephrology's 0by25 initiative for acute kidney injury (zero preventable deaths by 2025): a human rights case for nephrology. The Lancet. 2015;385(9987):2616-43.

Singu B. Set Plasma concentration and eGFR in preterm and term neonates receiving gentamicin or successive amikacin therapy. BMC pedia. 2023;23(1):24.

Duffy CR. Clindamycin, gentamicin, and risk for clostridium difficile infection and acute kidney injury during delivery hospitalizations. Obstet Gynecol. 2020;135(1):59.

Wu J. Effect of curcumin on glycerol-induced acute kidney injury in rats. Scientific reports, 2017;7(1):101-14.

Jewo P. The protective role of ascorbic acid in burn-induced testicular damage in rats. Burns. 2012;38(1):113-9.

Selby NM. Gentamicin-associated acute kidney injury. QJM. Int J Med. 2009;102(12):873-80.

Cui X. Acute kidney injury in patients with the coronavirus disease 2019: a multicenter study. Kidney Blood Press. 2020;45(4):612-22.

Ali B. Curcumin has a palliative action on gentamicin‐induced nephrotoxicity in rats. Fundam Clin Pharmacol. 2005;19(4):473-7.

De Oliveira JR, Camargo SEA, De Oliveira LD. Rosmarinus officinalis L (rosemary) as therapeutic and prophylactic agent. J Biomed Sci. 2019;26(1):5.

Ghaznavi H. Ameliorative effects of gallic acid on gentamicin-induced nephrotoxicity in rats. J Asian Nat Prod Res. 2018;20(12):1182-93.

Jafaripour L. Effects of rosmarinic acid on methotrexate-induced nephrotoxicity and hepatotoxicity in wistar rats. Indian J Nephrol. 2021;31(3):218.

Parasuraman SR, Raveendran R, Kesavan. Blood sample collection in small laboratory animals.JPP. 2010;1(2):87.

Liu B. Reversible acute kidney injury associated with sildenafil overdose. Cureus. 2018;10:9.

Hassanen NH. Protective effect of rosemary (Rosmarinus officinalis) against diethylnitrosamine-induced renal injury in rats. Biomarkers. 2020;25(3):281-9.

Sakr SA, Lamfon HA. Protective effect of rosemary (Rosmarinus officinalis) leaves extract on carbon tetrachloride-induced nephrotoxicity in albino rats. Life Sci J. 2012;9(1):779-85.

Kata FS. Short-time effects of malathion pesticide on functional and Histological changes of liver and kidney in female mice. PJBS. 2020;23(9):1103-12.

Kundu NK. Studies of lipid profile, liver function and kidney function parameters of rat plasma after chronic administration of sulavajrini vatika. PJBS. 2012;15(14):666-72.

Ribeiro, M.From the distinctive smell to therapeutic effects: Garlic for cardiovascular, hepatic, gut, diabetes and chronic kidney disease. Clinical Nutrition. 2021;40(7):4807-19.

Rafieian-Kopaei M, Baradaran A. Combination of metformin with other antioxidants may increase its renoprotective efficacy. JRIP. 2013;2(2):35.

Downloads

Published

2024-08-28

How to Cite

Waswa, K. W., & Kori, S. (2024). Renal biomarkers of Rosemarinus officinalis L. on gentamicine induced acute kidney injury in adult male albino rats (Rattus norvegicus). International Journal of Basic & Clinical Pharmacology, 13(5), 587–592. https://doi.org/10.18203/2319-2003.ijbcp20242414

Issue

Section

Original Research Articles