Evaluation of invitro antidiabetic activity using silver nanoparticles of Galinsoga parviflora leaf extract
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20242420Keywords:
Traditional medicine, Silver nanoparticles, α-Glucosidase inhibition, Antidiabetic activity, G. parviflora, Glucose uptake, α-Amylase inhibitionAbstract
Background: Diabetes mellitus, characterized by chronic hyperglycemia, can lead to severe complications if untreated. G. parviflora, a plant from the Astereaceae family, is traditionally recognized for its medicinal properties, including hypoglycemic effects. This study investigates the antidiabetic potential of silver nanoparticles synthesized from G. parviflora leaf extract. To evaluate the in-vitro antidiabetic activity of silver nanoparticles from G. parviflora by assessing their α-amylase and α-glucosidase inhibitory activities, and glucose uptake using the everted gut sac method.
Methods: Silver nanoparticles were synthesized from G. parviflora and tested for α-amylase and α-glucosidase inhibitory effects using colorimetric methods. Glucose uptake activity was assessed with an everted gut sac model from chicken ileum. Statistical analysis employed one-way ANOVA and Tukey’s post-hoc test, with significance set at p<0.05.
Results: The nanoparticles exhibited significant α-amylase inhibitory activity, with 90.2% inhibition at 200 µl and 91.9% at 400 µl, surpassing the standard drug Acarbose. They also showed dose-dependent α-glucosidase inhibitory activity, achieving 34.5% inhibition at 200 µl and 32.6% at 400 µl. Glucose uptake analysis revealed significant reductions at 150 minutes for both the standard drug and nanoparticles compared to the control (p<0.05). However, at 300 minutes, only the standard treatment maintained significant differences.
Conclusions: Silver nanoparticles of G. parviflora exhibit significant in vitro antidiabetic activity by inhibiting key digestive enzymes and reducing glucose uptake. These findings suggest their potential for developing novel antidiabetic therapies.
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References
K.D Tripathi essentials of medical Pharmacology edition 6th JP, New Delhi. 2003.
American Diabetes Association, 2010a. Diabetes 2010 Vital statistics. Alexandra,VA: ADA.
Kitabchi AE, Umpierrez GE, Miles JM, Fisher JN. "Hyperglycemic crises in adult patients with diabetes". Diabetes Care. 209;32(7):1335-43.
"Diabetes". World Health Organization. archived from the original on 2023. Retrieved 2023.
Boulos L. Flora of Egypt, (VerbenaceaeCompositae). AlHadara Publishing, Cairo, Egypt. 2002;233-4.
Surywanshi V, Yadava RN. New potential allelochemicals from Galinsoga parviflora CAV. Chem Sci Rev Lett 2015; 4(13):405-13.
Bazylko A, Stolarczyk M, Derwinska M, Kiss AK. Determination of antioxidant activity of extracts and fractions obtained from Galinsoga parviflora and Galinsoga quadriradiata, and a qualitative study of the most active fractions using TLC and HPLC methods. Nat Prod Res. 2012;26(17):1584-93.
Hamill FA, Apio S, Mubiru, NK, Mosango M, Bukenya ZR, Maganyi, OW, Soejarto, DD. Traditional herbal drugs of southern Uganda, I. J Ethnopharmacol. 2000;70(3):281-300.
Agra MD, Baracho GS, Nurit K, Basílio IJ, Coelho VP. Medicinal and poisonous diversity of the flora of “CaririParaibano”. J Ethnopharmacol. 2007;111(2):383-95.
Tolossa, K, Debela, E, Athanasiadou, S, Tolera, A, Ganga, G, Houdijk, JGM, Ethno-medicinal study of plants used for treatment of human and livestock ailments by traditional healers in South Omo, Southern Ethiopia. J Ethnobiol Ethnomed. 2013;9:32.
Bazylko, A, Boruc, K, Borzym, J, Kiss, AK, Aqueous and ethanolic extracts of Galinsoga parviflora and Galinsoga ciliata-composition investigations using HPTLC and HPLC-DAD-MS methods. Phytochem Lett. 2015;11:394-8.
Pande PC, Tiwari L, Pande HC. Ethnoveterinary plants of Uttaranchal- a review. Indian J Trad Know 2007;6(3):444-58.
Watt JM, Breyer-Brandwijk MG. The medicinal and poisonous plants of southern Africa and eastern Africa. Livingstone. 1962;1457.
Mostafa I, El-aziz EA, Hafez S, El-shazley A. Chemical constituents and biological activities of Galinsoga parviflora Cav. (Astraceae) from Egypt. Z. Naturforsch C Bio Sci. 2013;68(7-8):285-92.
Odhav B, Beekrum S, Akula US, Baijnath H. Preliminary assessment of nutritional value of traditional leafy vegetables in KwaZulu-Natal, South Africa. J Food Comp Anal. 2007;20(5):430-5.
Vainio-Mattila K. Wild vegetables used by the Sambaa in the Usambara Mountains, NE Tanzania. Ann Bot Fenn. 2000; 37:57-67.
Plummer, D. An introduction to practical biochemistry. McGraw-HILL, London.1978.
Sagbo IJ, van de Venter M, Koekemoer T, Bradley G. In vitro antidiabeticactivity and mechanism of action of Brachylaena elliptica (Thunb.) DC evidence based complementary and alternative medicine. 2018;1-13.
Geetha K Damodaran, Practical Biochemistry. Jaypee Brothers. Medical Publishers Pvt. Limited. 2010.
Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Biochem. 1969;6(1):24-7.
Sekar V, Chakraborty S, Mani S, Sali VK, Vasanthi HR. Mangiferin from Mangifera indica fruits reduces post-prandial glucose level by inhibiting alphaglucosidase and alpha-amylase activity. S Afr J Bot. 2019;120:129-34.
Therasa SV, Thirumalai T, Tamilselvan N, David E. In-vivo and ex-vivo inhibition of intestinal glucose uptake: a scope for anti-hyperglycemia. J Acute Dis. 2014;3;1-3.
Ferheen S, Rehman AU, Afza N, Malik A, Iqbal L, Azam RM, Irfan AM, Bakhsh TR. Galinsosides A and B, bioactive flavanone glucosides from G. parviflora. J Enzyme Inhib Med Chem 2009;24(5):1128-32.
Mostafa I, El-aziz EA, Hafez S, El-shazley A. Chemical constituents and biological activities of Galinsoga parviflora Cav. (Astraceae) from Egypt. Z Naturforsch C Bio Sci. 2013;68(7-8):285-92.
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