Assessment of antimicrobial activity of Meliponula ferrugica in pathogenic wound samples

Authors

  • Jasmin Sajini Rajayan Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, SRIHER, Porur, Chennai, Tamil Nadu, India
  • Naveen Rangasamy Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, SRIHER, Porur, Chennai, Tamil Nadu, India
  • Niranjan Lakshmi Narayan Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, SRIHER, Porur, Chennai, Tamil Nadu, India
  • Vijila Helen Mary George Department of Microbiology, Malankara Catholic College, Kanyakumari District, Tamil Nadu, India
  • Karthik Rajendran Department of Bioanalytical, Scitus Pharma Services Private Limited, Thirumazhisai, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20241652

Keywords:

Antibacterial, Antifungal, Bactericidal, Bacteriostatic, Honey, Human infection, Wounds

Abstract

Background: Honey is a naturally occurring sweet material that bees make from nectar from flowers, secretions from plant parts, or excretions from plants that suck insects from plant parts. Honey’s antibacterial and antifungal qualities are widely known, and it has been used to heal burns, surgical wounds, and decubitus ulcers. Honey instantly sterilizes wounds affected by Staphylococcus aureus. The honey’s ability to fight bacteria can be ascertained by the nectar’s origin.  Many antibiotic resistances among the bacteria that cause infections in humans have directly evolved as a result of the use of antibiotics in clinical practice.

Methods: This study found that Bergey’s manual of determinative bacteriology can be used to identify Pseudomonas aeruginosa and Staphylococcus aureus. According to the study’s findings, honey has a capacity to neutralise Pseudomonas aeruginosa and Staphylococcus aureus that have been isolated from infected wounds. Honey’s antibacterial characteristics account for a significant portion of its antibacterial activity.

Results: Staphylococcus aureus and Pseudomonas aeruginosa were both susceptible to the antibacterial activity of the honey sample. Pseudomonas aeruginosa exhibited lower activity compared to Staphylococcus aureus.

Conclusions: The results of this investigation show that Bergey’s handbook of determinative bacteriology can be used to identify Staphylococcus aureus and Pseudomonas aeruginosa. According to the study’s findings, honey has the ability to neutralise Pseudomonas aeruginosa and Staphylococcus aureus that have been isolated from infected wounds.

Metrics

Metrics Loading ...

References

Codex Alimentarius (Codex). Report of the 24th Session of the Joint FAO/WHO Codex Alimentarius Commission. ALINORM 01/41. In: Joint FAO/WHO Codex Alimentarius Commission, 24th session. July 2-7, Geneva, Switzerland. Codex Alimentarius, Rome, Italy; 2001

Al-Waili NS. Natural honey lowers plasma glucose, C-reactive protein, homocysteine, and blood lipids in healthy, diabetic, and hyperlipidemic subjects: comparison with dextrose and sucrose. J Med Food. 2004;7(1):100-7.

Subrahmanyam M. Topical application of honey for burn wound treatment- an overview. Ann Burns Fire Disast. 2007;20(3):137-9.

Subrahmanyam M. Topical application of honey in the treatment of burns. Br J Surg. 1991;78:497-8.

Farouk A, Hassan T, Kashif H, Khalid SA, Mutaawali I, Adi MW. 1988. Studies on Sudanese bee honey: Laboratory and Clinical Evaluation. Int J Crude Drug Res. 1988;26(3):161-8.

Levy SB, Marshall B. Antibacterial resistance worldwide: causes, challenges and responses. Nat Med. 2004;10(12):S122-9.

Molan PC. The antibacterial activity of honey. Bee World. 1992;73:5-28.

Rubin RH. Surgical wound infection: epidermology, pathogenesis, diagnosis and management. BMC Infect Dis. 2006;6:171-2.

Brook I. (1996) Aerobic and anaerobic microbiology of necrotizing fasciitis in children. Pediatr Dermantol. 1996;13(4):281-4.

Lee H, Churey JJ, Worobo RW. Antimicrobial activity of bacterial isolates from different floral sources of honey. Int J Food Microbiol. 2008;126(1-2):240-4.

Mandal S, Mandal MD, Pal NK. Antimicrobial resistance pattern of Salmonella typhi isolates in Kolkata, India during 1991-2001: a retrospective study. Japan J Infect Dis. 2002;55(2):58-9.

Mandal S, Deb Mandal M, Pal NK. Synergistic anti-Staphylococcus aureus activity of amoxicillin in combination with Emblica officinalis and Nymphae odorata extracts. Asian Pac J Trop Med. 2010;3:711-4.

Tan HT, Rahman RA, Gan SH, Halim AS, Hassan SA, Sulaiman SA, et al. The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complement Altern Med. 2009;9:34.

Lusby PE, Coombes AL, Wilkinson JM. Bactericidal activity of different honeys against pathogenic bacteria. Arch Med Res. 2005;36(5):464-7.

Basson NJ, Grobler SR. Antimicrobial activity of two South African honeys produced from indigenous Leucospermum cordifolium and Erica species on selected micro-organisms. BMC Complement Altern Med. 2008;8:41.

Chauhan A, Pandey V, Chacko KM, Khandal RK. Antibacterial activity of raw and processed honey. Electron J Biol. 2010;5:58-66.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniiques of plant analysis. 3rd edn. London: Chapman and Hall Publication; 1992:22-26.

Thompson J. A revision of the genus Leptospermum (Myrtaceae). National herbarium of New South Wales, Royal Botanic Gardens, Sydney, Australia. Telopea. 1988;3:301-449.

Macrae R, Robinson RK, Saler MJ. Saponins. In: Food Science, Food Technology and Nutrition. Vol 6. New York: Academic Press Ltd; 1993.

Willix DJ, Molan PC, Harfoot CG. A comparison of the sensitivity of wound‐infecting species of bacteria to the antibacterial activity of manuka honey and other honey. J Appl Bacteriol. 1992;73(5):388-94.

Selcuk H, Nevin K. Investigation of antimicrobial effect of honey collected from various regions of turkey. Pak J Biol Sci. 2002;5(3):325-8.

Subrahmanyam M, Hemmady AR, Pawar SG. Antibacterial activity of honey on bacteria isolated from wounds. Ann Bums Fire Disast. 2001;14(1):198-201.

Molan P. The evidence and the rationale for the use of honey as a wound dressing. Wound Pract Res. 2011;19(4):201-21.

Basualdo C, Sgroy VS, Finola MM, Marioli J. Comparison of the antibacterial activity of honey from different provenance against bacteria usually isolated from skin wounds. Veter Microbiol. 2007;124:375-81.

Bilal AN, Molan PC, Sallal AK. Antimicrobial activity of honey on selected microorganisms: a preliminary study. Biomed Res. 1998;9:51-4.

Alaux C, Ducloz F, Crauser D, Leconte Y. Diet effects on honeybee immune competence. Biol Lett. 2010;6(4):562-5.

Weston RJ, Mitchell KR, Allen KL. Antibacterial phenolic components of New Zealand manuka honey. Food Chem. 1999;64(3):295-301.

Majtan J, Kaludiny J, Bohova J, Kohutova L, Dzurova M. Methylglyoxal-induced modifications of significant honeybee proteinous components in manuka Honey: possible therapeutics implications. Fitoterapia. 2012;83(4):671-7.

Dustman JH. Antibacterial effect honey. Apiacta 1970;14:7-11.

Downloads

Published

2024-06-25

How to Cite

Rajayan, J. S., Rangasamy, N., Narayan, N. L., George, V. H. M., & Rajendran, K. (2024). Assessment of antimicrobial activity of Meliponula ferrugica in pathogenic wound samples. International Journal of Basic & Clinical Pharmacology, 13(4), 511–519. https://doi.org/10.18203/2319-2003.ijbcp20241652

Issue

Section

Original Research Articles