Combination of purple sweet potato (Ipomoea batatas L.) leaf extract with metformin on blood glucose and total cholesterol levels of albino rats induced by high-fat diet and streptozotocin

Authors

  • Marselina Saing Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Urip Harahap Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Panal Sitorus Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20240001

Keywords:

Diabetes mellitus, SPLE, Metformin, Total cholesterol

Abstract

Background: Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to impaired insulin secretion, often accompanied by hypercholesterolemia. Metformin is a first-line antihyperglycemic drug that is often combined with other antihyperglycemic drugs. Purple sweet potato leaves have been widely studied to reduce glucose and cholesterol levels.

Methods: This study was an experimental study using rats induced with a high-fat diet and streptozotocin, which were divided into 8 treatment groups, namely groups given CMC Na 0.5%, Metformin 45 mg/kg BW, Purple Sweet Potato Leaf Extract (SPLE) 200 mg/kg BW, SPLE 400 mg/kg BW, SPLE 800 mg/kg BW, SPLE 200 mg/kg BW with metformin 45 mg/kg BW, SPLE 400 mg/kg BW with metformin 45 mg/kg BW, and SPLE 800 mg/kg BW with metformin 45 mg/kg BW.

Results: After treatment for 28 days with SPLE doses of 200 mg, 400 mg, and 800 mg/kg BW, both single doses and combinations with metformin showed a decrease in fasting blood glucose levels and total cholesterol, which were statistically significantly different (p<0.05) between treatment groups using the one-way ANOVA. The combination of SPLE 800 mg/kg BW with metformin normalized blood glucose levels of 93.50±4.93 mg/dl.

Conclusion: The combination of purple sweet potato leaf extract with metformin is more effective in reducing blood glucose and total cholesterol levels compared to the single administration of metformin and SPLE.

References

Janapati YK, Junapudi S, Dachani SR. Optimization of Diabetes by Herbal Medicine. Technol Innovat Pharma Res. 2021;6:1-18.

International Diabetes Federation. Available at: https://diabetesatlas.org/idfawp/resource files/2021/07/IDF.pdf. Accessed on 20 February 2023.

Tan J, Kou J. Advances in Research on the Pathogenesis of Type 2 Diabetes Complicated with Gallstone. Int J Clin Med. 2019;10:161-73.

Dhoj TS, Raj KCS, Santosh G, Deepika G. Dyslipidemia in type 2 diabetes mellitus. J Pathol Nepal. 2017;7:1149-54.

Chen H, Li R. Introduction of Diabetes Mellitus and Future Prospects of Natural Products on Diabetes Mellitus. In: Structure and Health Effects of Natural Products on Diabetes Mellitus. Singapore: Springer; 2021:1-15.

Rahimi G, Heydari S, Rahimi B, Abedpoor N, Niktab I, Safaeinejad Z, et al. A combination of herbal compound (SPTC) along with exercise or metformin more efficiently alleviated diabetic complications through down-regulation of stress oxidative pathway upon activating Nrf2-Keap1 axis in AGE rich diet-induced type 2 diabetic mice. Nutr Metab (Lond). 2021;18(1):14.

Rena G. Hardie DG, Pearson ER. The mechanisms of action of metformin. Divi Mole Clin Med. 2017;16: 1165-70.

Flory J, Lipska K. Metformin. JAMA; 2019;321(19): 1926-7.

Roxo DF, Arcaro CA, Gutierres VO, Costa MC, Oliveira JO, Lima TFO, et al. Curcumin combined with metformin decreases glycemia and dyslipidemia, and increases paraoxonase activity in diabetic rats. Diabetol Metab Syndr. 2019;11:33.

Nguyen HC, Chen CC, Lin KH, Chao PY, Lin HH, Huang MY. Bioactive Compounds, Antioxidants, and Health Benefits of Sweet Potato Leaves. Molecules. 2021;26(7):1820.

Naomi R, Bahari H, Yazid MD, Othman F, Zakaria ZA, Hussain MK. Potential Effects of Sweet Potato (Ipomoea batatas) in Hyperglycemia and Dyslipidemia-A Systematic Review in Diabetic Retinopathy Context. Int J Mol Sci. 2021;22(19):10816.

Novrial D, Soebowo S, Widjojo P. Protective effect of Ipomoea batatas L leaves extract on histology of pancreatic langerhans islet and beta cell insulin expression of rats induced by streptozotocin. Molekul. 2020;15:48-55.

Husna F, Suyatna FD, Arozal W, Furwaningsih FH. Animal Model in Diabetes Research. Pharma Sci Res. 2019; 6(3):131-41.

Rafiu AA, Luka CD. Effect of Aqueous Sweet Potato Leaf Extract and Metformin on Some Biochemical Parameters in Streptozotocin- Induced Diabetic Rats. Am Sci Res J Engineer Technol Sci. 2018;39(1):82-92.

Zhang C, Liu D, Wu L, Zhang J, Li X, Wu W. Chemical Characterization and Antioxidant Properties of Ethanolic Extract and Its Fractions from Sweet Potato (Ipomoea batatas L.) Leaves. Foods. 2019;9(1):15.

Zhang L, Tu ZC, Yuan T, Wang H, Xie X, Fu ZF. Antioxidants and α-glucosidase inhibitors from Ipomoea batatas leaves identified by bioassay-guided approach and structure-activity relationships. Food Chem. 2016;208:61-7.

I'tishom R, Wafa IA, Budi DS. Oral delivery of purple sweet potato (Ipomoea batatas L.) Extract-Loaded Carboxymethyl Chitosan and Alginate Nanocapsule in Streptozotocin-induced Diabetic Mice. Indian J Pharma Edu Res. 2021;55(3):709-14.

El Barky AR, Hussein SA, Alm-Eldeen AA. Anti-diabetic activity of Holothuria thomasi saponin. Biomed. Pharmacother. 2016;84:1472-87.

Ntchapda F, Tchatchouang FC, Miaffo D, Maidadi B, Vecchio L, Talla RE, et al. Hypolipidemic and anti-atherosclerogenic effects of aqueous extract of Ipomoea batatas leaves in diet-induced hypercholesterolemic rats. J Integr Med. 2021;19(3):243-50.

Mbouche FMJ, Tatsadjieu NL, Ndjouenkeu R. Ipomea batatas Leaf Powder from Cameroon: Antioxidant Activity and Antihyperlipidemic Effect in Rats Fed with a High-Fat Diet. J Lipids. 2021;2021:5539878.

Kurata R, Kobayashi T, Ishii T, Niimi H, Niisaka S, Kubo M. Influence of sweet potato (Ipomoea batatas L.) leaf consumption on rat lipid metabolism. Food Sci Technol Res. 2017;23(1):57-62.

Nurkhasanah M, Nanik S, Muhammad S. The antihyperlipidaemic and hepatoprotective effect of Ipomoea batatas L. leaves extract in high-fat diet rats. Int J Public Health Sci. 2021;10:558.

Xiao N, Lou MD, Lu YT, Yang LL, Liu Q, Liu B, et al. Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice. Diabetologia. 2017;60(6):1084-93.

Nam SJ, Han YJ, Lee W, Kang B, Choi MK, Han YH, et al. Effect of Red Ginseng Extract on the Pharmacokinetics and Efficacy of Metformin in Streptozotocin-Induced Diabetic Rats. Pharmaceutics. 2018;10(3):80.

Downloads

Published

2024-01-02

How to Cite

Saing, M., Harahap, U., & Sitorus, P. (2024). Combination of purple sweet potato (Ipomoea batatas L.) leaf extract with metformin on blood glucose and total cholesterol levels of albino rats induced by high-fat diet and streptozotocin. International Journal of Basic & Clinical Pharmacology, 13(2), 203–207. https://doi.org/10.18203/2319-2003.ijbcp20240001

Issue

Section

Original Research Articles