Antifungal activity testing of curry leaf ethanol extract on the growth of Pityrosporum ovale and Candida albicans fungus

Authors

  • Ulwan Purnama Sari Postgraduate Program of Pharmaceutical Science, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Julia Reveny Departement of Pharmaceutical Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Siti Morin Sinaga Departement of Chemistry Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20233892

Keywords:

Pytrosporum ovale fungus, Candida albicans fungus, MIC, MBC, Curry leaf

Abstract

Background: The curry leaf is an Indonesian plant commonly utilized as a spice. Curry leaves are abundant in secondary metabolites, which endow this plant with numerous advantages, including antibacterial and antifungal properties, as well as the ability to reduce blood sugar levels and blood pressure. The objective of this study is to assess the efficacy of an ethanol extract derived from curry leaves in suppressing the proliferation of Pityrosporum ovale and Candida albicans fungus.

Methods: The symbiotic properties and phytochemical composition of curry leaf simplisia were examined. The antifungal efficacy of the ethanol extract derived from curry leaves was evaluated against Pityrosporum ovale and Candida albicans using the disc diffusion method. Calculate the precise values of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC).

Results: The simplisia of curry leaf fulfills the criteria for simplisia characterization. Curry leaves possess a variety of secondary metabolite chemicals, including alkaloids, flavonoids, tannins, saponins, glycosides, steroids, and triterpenoids. The activity against the inhibition of fungal growth of Pityrosporum ovale and Candida albicans can suppress fungal growth with inhibition zone diameters measuring 6.93±0.15 mm and 7.27±0.47 mm, respectively. The minimum lethal concentration of leaf ethanol extract for Pityrosporum ovale fungi is 8.75%, resulting in a decrease of 98.25%. For Candida albicans fungi, the minimum lethal concentration is 12.5%, resulting in a reduction of 98.37%.

Conclusions: The ethanol extract derived from curry leaves has the ability to hinder the proliferation of Pityrosporum ovale and Candida albicans fungus.

References

Amalyuri AG, Reveny J, Dalimunthe A. Antibacterial Potential of Ethanol Extract of Tamarind Seed Bark (Tamarindus indica L.) And Formulation of Anti-Acne Nanogel. Int J Sci Technol Manag. 2022;3(3):598-604.

Simanjuntak HA, Butar-Butar M. Uji Aktivittas Antifungi Ekstrak Etanol Umbi Bawang Merah (Allium Cepa L.) Terhadap Candida albicans Dan Pityrosporum Ovale. J Penelitian Pembelajaran MIPA. 2019;4:79.

Rani Z, Nasution HM, Kaban VE, Nasri N, Karo NB. Antibacterial activity of freshwater lobster (Cherax quadricarinatus) shell chitosan gel preparation against Escherichia coli and Staphylococcus aureus. J Appl Pharmac Sci. 2022;13(2):146-53.

Talapko J, Martina J, Tatjana M, Emina P, Sanja B, Ivan K et al. Candida albicans-the virulence factors and clinical manifestations of infection. J Fungi. 2021;7(2):79.

Gozali D, Mustarichie R. Hair tonic formulation of anti-alopecia of Angiopteris evecta extract. Res J Pharmacy Technol. 2019;12:1079-85.

Joste V, Justine B, Véronique H, Cécile P, Mathieu G, Emilie G et al. Plasmodium ovale wallikeri and P. ovale curtisi Infections and Diagnostic Approaches to Imported Malaria, France, 2013-2018. Emerg Infect Dis. 2021;27(2):372-84.

Gadge SS, Wankhade SP, Tapare S, Kalaskar SM, Holey SD. Formulation and evaluation of polyherbal antidandruff shampoo. J Pharmacognosy Phytochem. 2023;12:35-41.

Lubis MF, Kaban VE, Aritonang JO, Satria D, Mulina AA, Febriani H. Acute toxicity and antifungal activity of the ointment Murraya koenigii ethanol extract. Rasayan J Chem. 2022;15(1):256-61.

Abuga I, Sulaiman SF, Wahab RA, Ooi KL, Rasad MSB. A. In vitro antibacterial effect of the leaf extract of Murraya koenigii on cell membrane destruction against pathogenic bacteria and phenolic compounds identification. Europ J Integrative Med. 2020;33:101010.

Ramnath GM, Jayaraman S, Thirugnanasampandan R, Gunasekaran B, Gopalakrishnan A. Chemical composition analysis and evaluation of antioxidant, antiacetylcholinesterase and cytotoxicity of Murraya koenigii (l.) Spreng fruit oil. J Microbiol Biotechnol Food Sci. 2023;e6135.

Suthar P, Satish K, Vikas K, Devina V, Ajay S, Ajit S. Murraya koenigii (L.) Spreng: Speculative ethnobotanical perspectives of ubiquitous herb with versatile nutra/functional properties. S Afri J Botany. 2022;145(9):111-34.

Hidayanti N, Yusro F, Mariani Y. Bioaktivitas Minyak Daun Kari (Murraya koenigii (L.) Spreng Terhadap Bakteri Enterococcus faecalis dan Salmonella typhimurium. J Biologi Makassar. 2020;5:95-102.

Gurning K. Characterization and Screening of Phytochemical Secondary Metabolite of Seri (Muntingia calabura, L) Leaves which is Potential as an Anti-Diabetic based on Indonesian Herbal Medicine Standard. J Drug Delivery Therapeut. 2020;10:92-4.

Owuama CI. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. Afr J Microbiol Res. 2017;11(23):977-80.

Muhammad M, Nasri N, Kaban VE, Satria D, Cintya H. Antibacterial Potential Ethanol Extract of Papaya Leaves (Carica papaya Linn.) Towards Salmonella typhi. J Biol Educat Sains Technol. 2022;5(2):265-70.

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharmaceutical Analysis. 2016;6(2):71-9.

Shivanna VB, Subban N. Effect of various drying methods on flavor characteristics and physicochemical properties of dried curry leaves (Murraya koenigii L. Spreng). Drying Technol. 2014;32(8):882-90.

Ikhsan MW. Aktivitas antibakteri dan analisis bioautografi ekstrak etanol daun kari (Murraya koenigil (l) spreng) terhadap bakteri Propionibacterium acnes. Uumn al-washliyah 48 FAR. 2022.

Vats M, Singh H, Sardana S. Phytochemical screening and antimicrobial activity of roots of Murraya koenigii (Linn.) Spreng. (Rutaceae). Braz J Microbiol. 2011;42(2):1569-73.

Harbone JB. Phytochemical Methods London, 3rd edi. Chapman and Hall publications. 1973.

Bassino E, Gasparri F, Munaron L. Protective role of nutritional plants containing flavonoids in hair follicle disruption: A review. Int J Molecular Sci. 2020;21:523.

Ryu SY, Kim SD, Jang SY. Antifungal activity of plant extracts against Pityrosporum ovale and Candida albicans. Kor J Pharmacognosy. 2003;34(4):303-7.

Takahashi M, Ushijima T, Ozaki Y. Biological activity of Pityrosporum. II. Antitumor and immune stimulating effect of Pityrosporum in mice. J National Cancer Institute. 1986;77(5):1093-7.

Gurning K, Siahaan D, Iksen I. Antibacterial Activity Test of Extract Ethanol of Jackfruit Leaves (Artocarpus heterophyllus. Lamk.) of Bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis and Salmonella typhi. J Pharmaceutical Sci. 2019;2(2):49-54.

Septama AW, Panichayupakaranant P. Antibacterial assay-guided isolation of active compounds from Artocarpus heterophyllus heartwoods. Pharmaceutical Biol. 2015;53(11):1608-13.

Satria D, Sofyanti E, Wulandari P, Pakpahan SD, Limbong SA. Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC® 25175TM and Staphylococcus aureus ATCC® 6538TM. Pharmacia. 2022;69:195-202).

Mardiana RN, Handayani N. Antibacterial activity of the sambiloto leaf extracts (Andrographis paniculata) to Bacillus cereus and Pseudomonas aeruginosa. Asian J Natural Product Biochem. 2016;14:19-24.

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Published

2023-12-26

How to Cite

Purnama Sari, U., Reveny, J., & Morin Sinaga, S. (2023). Antifungal activity testing of curry leaf ethanol extract on the growth of Pityrosporum ovale and Candida albicans fungus. International Journal of Basic & Clinical Pharmacology, 13(1), 57–63. https://doi.org/10.18203/2319-2003.ijbcp20233892

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Original Research Articles