Optimization of nutrient media for glucoamylase production by Plackett-Burman design using Aspergillus niger

Srivani Gaddam

doi:10.18203/2319-2003.ijbcp20233904

Authors

Srivani Gaddam Pullareddy Institute of Pharmacy, Dundigal, Hyderabad, Telangana, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20233904

Keywords:

Wheat bran, Plackett-Burman design, Centrifugation

Abstract

Background: Glucoamylase is an important enzyme produced by various bacterial and fungal species as an intracellular or extra cellular enzyme. It is used to treat gastrointestinal issues, autoimmune diseases, inflammation, psoriasis, allergies, eczema, emphysema and asthma, and thus, supplements are often included in complexes for digestive disorders and also applications in different industries.

Methods: Natural ingredients are screened for glucoamylase production by stationary culture of solid-state fermentation technique and Plackett-Burman design microorganism used Aspergillus niger. Eight nutrient medias are designed and from this glucoamylase isolated from the medium by centrifugation at 1500 rpm and assayed by spectrophotometric method.

Results: high glucoamylase levels are found in nutrient media VI with optimized physical conditions like percentage inoculum- 2%, pH- 6, incubation temperature- 40⁰C upon incubation for 4 days.

Conclusions: High amount of glucoamylase is produced in the media VI due to the presence of high amounts of sucrose and optimum concentration of potato, peptone, wheat bran and yeast extract.

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