A comparative study of thiamine with metformin on fasting blood glucose of diabetic albino rats


  • Subhankar Choudhury Department of Pharmacology and Therapeutics, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India
  • Bhulan Prasad Loc Department of Pharmacology and Therapeutics, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India
  • Rati Ranjan Debbarma Department of Pharmacology and Therapeutics, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India
  • Arijit Das Department of Pharmacology and Therapeutics, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India




Thiamine, Diabetes mellitus, Streptozotocin, Fasting blood glucose


Background: Thiamine is a member of the vitamin B family. Thiamine is necessary for normal insulin synthesis and secretion. In diabetes thiamine and its derivative benfotiamine showed promising results in prevention of microvascular complications. Some experimental and clinical studies have shown the antihyperglycaemic effect of thiamine. This study compared the antihyperglycaemic effect of thiamine with metformin in streptozotocin-nicotinamide induced diabetic albino rats.

Methods: 24 albino rats were taken and divided into four groups of six rats in each group. The groups were normal control, diabetic control, diabetic rats treated with thiamine, diabetic rats treated with metformin. Diabetes was induced in three groups by intraperitoneal injection of Streptozotocin in the dose of 60 mg/kg. To have an ideal type 2 diabetes model nicotinamide was administered 120 mg/ kg intraperitoneally fifteen minutes before streptozotocin administration. After successful induction of diabetes thiamine and metformin were given to the respective group for a period of 6 weeks. Fasting blood glucose was estimated on day 0, 7, 14, 21, 28, 35, 42 of treatment.

Results: In this study both thiamine and metformin showed significant antihyperglycaemic effect (p<0.05). Further studies are needed to evaluate and compare the antihyperglycaemic effect of thiamine with other established anti diabetic drugs.

Conclusions: From this study we concluded that individually both thiamine and metformin were effective in controlling hyperglycaemia but metformin was better in achieving normal mean FBS. Further studies are required to validate the antihyperglycaemic effect of thiamine. Study taking different doses of thiamine or with increasing the duration of study period can elaborate the role of thiamine in achieving proper glycemic control.


Zimmet P. Preventing diabetic complications: a primary care perspective. Diabetes Res Clin Pract. 2009;84:107-16.

Raheja BS, Kapur A, Bhoraskar A, Sathe SR, Jorgensen LN, Moorthi SR, et al. Diab Care Asia: India Study: diabetes care in India - current status. J Assoc Physicians India. 2001;49:717-22.

Goodman Gillman. Insulin, Oral Hypoglycaemic agents and the pharmacology of endocrine pancreas. The Pharmacological Basis of Therapeutics Eleventh Edition. 2006;60:1686-10.

Gubler CJ, Thiamine, Handbook of Vitamins: Nutritional, Biochemical, and Clinical Aspects (L. J. Machlin, ed.), Marcel Dekker, New York; 1984:233.

Rathanaswami P, Pourany A, Sundaresan R. Effects of thiamine deficiency on the secretion of insulin and the metabolism of glucose in isolated rat pancreatic islets. Biochem Int. 1991;25(3):577-83.

Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature. 2001;414:813-20.

Hammes HP, Du X, Edelstein D. Thiamine and benfotiamine blocks three major pathways of hyperglycaemic damage and prevents experimental diabetic retinopathy. Nat Med. 2003;9:294-99.

Larkin JR, Thornalley PJ. High glucose causes a decrease in expression of thiamine transporters in human proximal tubule epithelial cells in vitro. Diabetologia. 2008;51:219.

Hoyumpa AM, Strickland R, Sheehan JJ, Yarborough G, Nichols S. Dual system of intestinal thiamine transport in humans. J Lab Clin Med. 1982;99(5):701-8.

Thornalley PJ, BJ R, Karachalias N, Rabbani N. Prevention of decline in glycaemic control in streptozocin-induced diabetic rats by thiamine but not by Benfotiamine. Diabet Med. 2010;27(1):74.

Mehdi JRK, Reza KH, Saba M, Kamilia M. Effect of supplementary consumption vitamin B1 (thiamine) on blood glucose changes during and after maximal aerobic exercise. ISSN. 2013;3(7):195-201.

Mohan C. Calbiochem buffers. A guide for the preparation and use of buffers in biological systems. 2014;1:17-22.

Nayak. Antidiabetic activity of benzopyrone analogues in nicotinamide-streptozotocin induced type 2 diabetes in rats. The Scientific World Journal Volume. 2014;2014:854267.

Sadek KM, Taha N, Korshom M, Mandour A. Thiamine ameliorate hepatic, renal dysfunction and dyslipidemia in diabetic rats. Journal of current research in science. 2013;1(1):35-9.

Rao AS. Pharmacological screening methods and toxicology. First edition. 2014;1:42-56.

Pacal L, Tomandl J, Svojanovsky J, Krusova D, Stepankova S, Rehorova J, et al. Role of thiamine status and genetic variability in transketolase and other pentose phosphate cycle enzymes in the progression of diabetic nephropathy. Nephrol Dial Transplant. 2011;26(4):1229-36.

Beltramo E, Berrone E, Tarallo S, Porta M. Effects of thiamine and benfotiamine on intracellular glucose metabolism and relevance in the prevention of diabetic complications. Acta Diabetol. 2008;45:131-41.

Pandhiani MB. Effect of thiamine on glycemic control in induced diabetic rat model. Journal of current research in science. 2013;1(1):68-74.

Gonzalez-Ortiz M, Martinez-Abundis E, Robles-Cervantes JA, Ramirez-Ramirez V, Ramos-Zavala MG. Effect of thiamine administration on metabolic profile, cytokines and inflammatory markers in drug-naive patients with type 2 diabetes. Eur J Nutr. 2011;50:145-9.

Smithline HA, Donnino M, Greenblatt DJ. Pharmacokinetics of high-dose oral thiamine hydrochloride in healthy subjects. BMC Clinical Pharmacology. 2012;12:4.




How to Cite

Choudhury, S., Loc, B. P., Debbarma, R. R., & Das, A. (2017). A comparative study of thiamine with metformin on fasting blood glucose of diabetic albino rats. International Journal of Basic & Clinical Pharmacology, 5(4), 1539–1543. https://doi.org/10.18203/2319-2003.ijbcp20162468



Original Research Articles