Evaluation of minimum inhibitory concentration of methanol extract of cacao seeds on growth of Streptococcus mutans bacteria by microdilution method

Authors

  • Cut Aja Nuraskin Poltekkes Kemenkes Aceh Jl. Soekarno Hatta, Tingkeum, Darul Imarah, Lheu Blang, Banda Aceh, Aceh Besar District, Aceh, Indonesia
  • T. Iskandar Faisal Poltekkes Kemenkes Aceh Jl. Soekarno Hatta, Tingkeum, Darul Imarah, Lheu Blang, Banda Aceh, Aceh Besar District, Aceh, Indonesia
  • Reca Poltekkes Kemenkes Aceh Jl. Soekarno Hatta, Tingkeum, Darul Imarah, Lheu Blang, Banda Aceh, Aceh Besar District, Aceh, Indonesia
  • Teuku Salfiyadi Poltekkes Kemenkes Aceh Jl. Soekarno Hatta, Tingkeum, Darul Imarah, Lheu Blang, Banda Aceh, Aceh Besar District, Aceh, Indonesia
  • Ainun Mardiah Poltekkes Kemenkes Aceh Jl. Soekarno Hatta, Tingkeum, Darul Imarah, Lheu Blang, Banda Aceh, Aceh Besar District, Aceh, Indonesia
  • Arifin Ahmad Poltekkes Kemenkes Aceh Jl. Soekarno Hatta, Tingkeum, Darul Imarah, Lheu Blang, Banda Aceh, Aceh Besar District, Aceh, Indonesia
  • Rivansyah Suhendra Department of Information Technology, Faculty of Engineering, Universitas Teuku Umar, Aceh Barat, Indonesia

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20231110

Keywords:

Mouth washing, Laban leaves, Steeping water, Plaque accumulation

Abstract

Background: Indonesia is famous for its medicinal plants. Cocoa bean (Theobroma cacao L.) is a plant widely used as a traditional medicine alternative to cure diseases. Cocoa beans contain alkaloids, flavonoids, saponins and tannins which are antibacterial and have potential as antioxidants. Various dental and oral diseases that occur are caused by bacteria, one of which is Streptococcus mutans which causes dental caries. Bacteria that play an important role in the occurrence of dental caries is Streptococcus mutans. Dental caries can be prevented by chemically and mechanically maintaining oral hygiene using antiseptics that have antibacterial properties. This study aimed to test the minimum inhibitory concentration of methanol extract of cocoa seeds on the growth of Streptococcus mutans with the microdilution method.

Methods: The research method is the microdilution method with concentrations of 0.753%, 1.563%, 3.125%, 6.25%, 12.5%, 25%, 50%, 100%. Streptococcus mutans bacteria were obtained from the University of Indonesia's veterinary laboratory.

Results: The results showed that the minimum inhibitory concentration of methanol extract of cocoa beans on the growth of Streptococcus mutans was at a concentration of 1.563%.

Conclusions: In conclusion, the methanol extract of cocoa beans has an inhibitory effect on Streptococcus mutans bacteria and there is a minimum inhibitory concentration at a concentration of 1.563%. It is recommended to continue to toothpaste medication and do preclinical trials.

References

Chassagne F, Hul S, Deharo E, Bourdy G. Natural remedies used by Bunong people in Mondulkiri province (Northeast Cambodia) with special reference to the treatment of 11 most common ailments. J Ethnopharmacol. 2016;191:41-70.

Hafidhah N, Hakim RF, Fakhrurrazi F. Pengaruh ekstrak biji kakao (Theobroma cacao L.) terhadap pertumbuhan enterococcus faecalis pada berbagai konsentrasi. J Caninus Denstistry. 2017;2(2):92-6.

Purnamasari DA, Munadziroh E, Yogiartono RM. Konsentrasi ekstrak biji kakao sebagai material alam dalam menghambat pertumbuhan Streptococcus mutans. PDGI. 2010;59(1):14-8.

Sari DP, Pangemanan DHC, Uji J. Daya hambat ekstrak alga coklat (Padina australis Hauck) terhadap pertumbuhan bakteri Porphyromonas gingivalis secara in vitro. e-GIGI. 2016;4(2):23-9.

Iflahah MA, Puspawati NM, Suaniti NM. Aktivitas antioksidan biji kakao (Theobroma cacao L.) Dalam menurunkan kadar 8-hidroksi- 2. Deoksiguanosin Dalam. 2016;4:113-9.

Ren Z, Chen L, Li J, Li Y. Inhibition of Streptococcus mutans polysaccharide synthesis by molecules targeting glycosyltransferase activity. J Oral Microbiol. 2016;8(1):25-8.

Nuraskin CA, Reca R, Salfiyadi T, Abdurrahman A, Faisal TI, Soraya C. Toothpaste activity test of laban leaf methanol extract (Vitex pinnata) against the growth of streptococcus mutans bacteria. Maced J Med Sci. 2021;9:95-100.

Chen L, Jia L, Zhang Q. A novel antimicrobial peptide against dental-caries-associated bacteria. Anaerobe. 2017;47:165-72.

Dewi ZY, Nur A, Hertriani T. Efek Antibakteri Dan Penghambatan Biofilm Ekstrak Sereh (Cymbopogon nardus L.) Terhadap Bakteri Streptococcus mutans. Kedokt Gigi Univ Gajah Mada. 2015;1(2):136-41.

Riskesdas K. Hasil Utama Riset Kesehata Da sar (RISKESDAS). J Physic. 2018;44(8):200.

Nuraskin CA, Faisal TI, . R, Salfiyadi T, Mardiah A. Investigation on the effectiveness of mouth-washing using laban leaves (Vitex pinnata) steeping water in reducing plaque accumulation: a study in state elementary school 1 Pagar Air, Aceh Besar. Int J Basic Clin Pharmacol. 2022;11(6):545.

Nuraskin CA. The effect of using Laban leaf (Vitex pinnata) methanol extract toothpaste on saliva pH in Students of SD Negeri I Pagar AIR. Int J Basic Clin Pharmacol. 2022;11(5):368.

Tobergte DR, Curtis S. perbedaan pasta gigi dengan dan tanpa tambahan ekstrak etanol daun belimbing wuluh (Averrhoa bilimbi L.) konsentrasi 10,5% terhadap hambatan pertumbuhan Streptococcus mutans. J Chem Inf Model. 2013;53(9):1689-99.

Nuraskin CA, Reca, Wirza AM. Effectiveness of guava leaf steep water against the bacterial growth of S. Mutans with Microdillution Method. Res J Pharm Technol. 2021;14(11):5745-8.

Zakki M. Uji Ekstrak Cathecin Teh Putih Terhadap bakteri S. mutans. Int J Basic Clin Pharmacol. 2017;4: 108-13.

Zakki M. Uji aktivitas antibakteri ekstrak cathechin teh putih terhadap. Res J Pharm Technol. 2016;5:43-8.

Khairi N, Aksa R, Berek Y. Uji efektifitas formula pasta gigi ekstrak daun binahong Anredera cordifolia (Ten.) Steenis). Sebagai Antiplak. 2016;2:32-6.

Crisminina. Efek Ekstrak Buah Jamblang Terhadap Pertumbuhan Streptococcus mutan sebagai penyebab utama karies. Dentica. 2011;16 (2):144-8.

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Published

2023-04-27

How to Cite

Nuraskin, C. A., Faisal, T. I., Reca, Salfiyadi, T., Mardiah, A., Ahmad, A., & Suhendra, R. (2023). Evaluation of minimum inhibitory concentration of methanol extract of cacao seeds on growth of Streptococcus mutans bacteria by microdilution method. International Journal of Basic & Clinical Pharmacology, 12(3), 340–344. https://doi.org/10.18203/2319-2003.ijbcp20231110

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Original Research Articles