Evaluation of the antiulcer and antimicrobial activities of methanol leaf extract of Helianthus annuus

Authors

  • Innocent F. Ajegi Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria
  • Gloria O. Ajegi Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria
  • Obinna C. Ajaegbu Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria
  • Matthew O. Nwokike Department of Pharmacology, Prince Abubakar Audu University, Anyigba, Nigeria
  • Mansur A. Ramalan Department of Pharmacology and Therapeutics, Bayero University, Kano, Nigeria
  • Victor I. Eje Department of Physiology, Federal University of Health Sciences, Otukpo, Nigeria
  • Godwin C. Akuodor Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nigeria

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20230381

Keywords:

Helianthus annuus, Leaf, Aspirin, Histamine, Microbes

Abstract

Background: Medicinal plants are widely used in treating and preventing specific diseases and are known to play an important role in health care. Helianthus annuus is one of such plants whose therapeutic applications no doubt have numerous folkloric background. This study aimed at assessing Helianthus annuus methanol leaf extract for antiulcer and antimicrobial potentials

Methods: The antiulcer activity was evaluated using aspirin, ethanol and histamine methods in Wistar rats, while the antimicrobial activity was carried out on selected microorganisms. The acute toxicity test and phytochemical screening of the extract were also conducted.

Results: The methanol leaf extract exhibited significant (p<0.05 and p<0.01) antiulcer effect in both model. The ulcer protection effect of the extract is comparable to omeprazole. The extract also significantly inhibited the growth of test organisms. The acute toxicity test produced no lethality in rats, whereas the phytochemical analysis revealed the presence of alkaloids, saponins, tannins, flavonoids, terpenoids and steroids.

Conclusions: The findings confirm the ethnomedicinal use of H. annuus leaf as a valuable natural agent for the treatment of ulcer and microbial infections.

References

Rakh MS, Chaudhari SR. Evaluation of CNS depressant activity of Momordica dioica Roxb willd fruit pulp. Int J Pharm Pharm Sci. 2010;2(4):124-6.

Gill NS, Bajwa J, Dhiman K, Sharma P, Sood S, Sharma PD, et al. Evaluation of therapeutic potential of traditionally consumed Cucumis melo seeds. Asian J Plant Sci. 2011;10(1):86-91.

Huang C, Zhou T, Chen Y, Zhang S, Chen G. Epicatechin regulation of hydroxysteroid sulfotransferase STa (rSULT2A1) Expression in Female Rat Steroidogenic Tissues. J Pharmacol Toxicol. 2011;6(4):349-60.

Akande IS, Kehinde EO, Adeshakin AO, Gbadero D. Effects of Faradin Natural Mixture on anaemia and anti-oxidant status of phenylhydrazine-induced anaemia in rats. Afr J Endocrinol Metabol. 2017;11(2):36-63.

Alam MB, Hossain MS, Chowdhury NS, Mazumder MEH, Haque ME, Islam A. In vitro and in vivo antioxidant and toxicity evaluation of different fractions of Oxalis corniculata Linn. J Pharmacol Toxicol. 2011;6(4):337-48.

Dwivedi A, Sharma GN, Kaushik AY. Evaluation of Helianthus annuus L. leaves extract for antidiarrheal and antihistaminic activity. Int J Res Ayurveda Pharm. 2015; 6(1):118-124

Onoja SO, Anaga AO. Bioassay-guided fractionation, antihyperglycemic and antioxidant properties of the methanol leaf extract of Helianthus annuus. Int J Pharmacog Phytochem Res. 2015;7(2):340-6.

Ejebe DE, Siminialayi IM, Emudainohwo JOT, Ofesi U, Morka L. Analgesic and anti-inflammatory activities of the ethanol extract of the leaves of Helianthus annuus in Wistar rats. Asian Pac J Trop Med. 2010;3:341-7.

Ejebe DE, Siminialayi IM, Nwadito C, Emudainowho JOT, Akonghrere R, Ovuakporaye SI. Effects of ethanol extract of leaves of Helianthus annuus on the reproductive system of male Wistar rats: Testicular histology, epididymal sperm properties, and blood levels of reproductive hormones. Biomed Pharmacol J. 2008;1:65-78.

Onoja SO, Anaga AO. Evaluation of the antidiabetic and antioxidant potentials of methanolic leaf extract of Helianthus annuus L. on alloxan-induced hyperglycemic rats. Comp Clin Pathol. 2014;23:1565-73.

Mahomoodally MF. Quantitative ethnobotanical study of common herbal remedies used against 13 human ailments catergories in Mauritius. Afr J Tradit Complement Altern Med. 2014;11(6):1-32.

Upadhyay RK, Ahmad S. Ethno-medicinal plants and their pharmaceutical potential. J Pharm Res. 2012;5(4):2162-73.

Onoja SO, Udem SC, Anaga AO. Effect of chronic administration of hydromethanol leaf extract of Helianthus annuus on erythrocytic profile in normal rats. J Nutr Ther. 2017;6(2):59-63.

Fatrcová-Šramková K, Nôžková J, Máriássyová M, Kačániová M. Biologically active antimicrobial and antioxidant substances in the Helianthus annuus L. bee pollen. J Environ Sci Health B. 2016;51(3):176-81.

Guide for the Care and Use of Laboratory Animals. 8th ed. Bethesda: NIH publisher; 2011: 82-3.

Harborne JB. Phytochemical methods. London: Chapman and Hall Ltd; 1978:49-188.

Adewunmi CO, Ojewole JAO. Safety of traditional medicines complementary and alternative medicine in Africa. Afr J Traditional Med. 2004;1:1-3

Lorke D. A new approach for acute toxicity testing. Arch Toxicol. 1983;54:275-87.

Akuodor GC, Essien AD, David-Oku E, Chilaka KC, Akpan JL, Ezeokpo B, Ezeonwumelu JOC. Gastroprotective effect of the aqueous leaf extract of Guiera senegalensis in Albino rats. Asian Pacific J Trop Med. 2013;3:771-5.

Akuodor GC, Idris-Usman M, Mbah CC, Megwas UA, Akpan JL, Ugwu TC, et al. Studies on anti-ulcer, analgesic and antipyretic properties of the ethanolic extract of Gongronema latifolium in rodents. Afr J Biotechnol. 2010;9: 2316-21.

Akuodor GC, Akpan JL, Ezeunala Mercy N, Ajoku Gloria A, Essien AD, Megwas AU, et al. Evaluation of anti-ulcer and antimicrobial effects of Verbena hastata leaf extract. Afr J Pharm Pharmacol. 2012;6(11):778-82.

Barrowman JA, Pfeifer CJ. Carbenoxolone a critical analysis of its clinical value in peptic ulcer: In drugs and peptic ulcer. CRC Press Boca Raton. 2012;3:123-32.

Megwas AU, Akuodor GC, Chukwu LC, Okorie EM, Desmond DO, Chukwumobi AN, Ikoro NC, Unekwe PC. Gastroprotective potentials of Annona senegalensis ethanol leaf extract in wistar rats. J Biol Stud. 2022;5(2):270-84.

Essiet GA, Anwankwo MU, Akuodor GC, Ajoku GA, Ofor CC, Megwas AU, et al. Antibacterial and toxicological evaluation of the ethanol leaf extract of Anthonotha macrophylla. J Herbmed Pharmacol. 2019;8(3):205-11.

Akuodor GC, Essiet GA, Ekenjoku JA, Udoh FV, Ogiji ED, Ibiam GA, Nnorom FO. Antidiarrhoeal and antimicrobial effects of ethanol root bark extract of Salacia lehmbachii. J Herbmed Pharmacol. 2021;10(2):188-93.

Gregory M, Vithalrao KP, Franhlin G, Kalaichelavan V. Ulcerpreventive activity of Ficus arnottiana miq(Moraceae) leaf methanolic extract. Am J Pharmacol Toxicol. 2009;4(3):89-93.

Darmani P, Palit G. Exploring Indian medicinal plants for antiulcer activity. Indian J Pharmacol. 2006;35:95-9.

Olaleye SB, Farombi EO. Attenuation of indomethacin and HCI/ ethanol-induced oxitative gastric mucosa damage in rats by Kolaviron: A natural biflavonoid of Garcina kola seed. Phytothera Res. 2006;20:14.

Akuodor GC, Udia PM, Akpan JL, Ezeokpo BC, Okezie OA, Ghasi SI, Nwadike KI. Ulcer protective effect of Byrsocarpus coccineus leaf extract in different experimental animal models. J Coastal Life Med. 2015;3(9):728-32.

Sen S, Chakraborty R, De B, Majumder M. Plants and phytochemicals for peptic ulcer: an overview. Pharmacog Rev. 2009;3:270-9.

Shah AH, Khan ZA. Gastroprotective effects of pretreatment with Zizyphussativa fruits against toxic damage in rats. Fitoterapia. 2014;3:226-34.

Essiet GA, Akuodor GC, Essien AD, Akpan JL, Asika EC, Nworie EM, Chilaka KC. Protective effects of salacia lehmbachii aqueous root bark extract againstgastric ulcer in wistar rats. Saudi J Med Pharma Sci. 2017;3(4):306-9.

Nwagba CA, Ezugwu CO, Eze CC, Anowi FC, Ezea SC, Nwakile CD. Anti-ulcer activity of Bombax buonopozense P. Beauv. aqueous leaf extract (Fam: Bombacaceae). J Appl Pharm Sci. 2013;3(2):139-42.

Amazu LU, Antia BS, Okokon JE. Antiulcerogenic activity of Solenostemon monostachyus. J Phytopharmacol. 2015;4(2):97-101.

Paguigan ND, Castillo DH, Chichioco-Hernandez CL. Anti-ulcer activity of leguminosae plants. Arq Gastroenterol. 2014;51(1):64-7.

Abebaw M, Mishra B, Gelayee D. Evaluation of anti-ulcer activity of the leaf extract of Osyris quadripartita Decne. (Santalaceae) in rats. J Experimenta Pharm. 2017;9:1-11

Maria D. In vitro and in vivo anti Helicobacter pylori activity of natural products, peptic ulcer disease; Arq Gastroenterol. 2011;23:978-53.

Sanyal AK, Banerjee CR, Das PK. Studies on peptic ulceration. Part II-role of banan in restraint and prednisolone-induced ulcer in albino rats. Arch Int Pharmacy. 2014;155:283-4.

Harris A, Misiewicz J. Helicobacter pylori. London: Blackwell healthcare; 1996:127.

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Published

2023-02-22

How to Cite

Ajegi, I. F., Ajegi, G. O., Ajaegbu, O. C., Nwokike, M. O., Ramalan, M. A., Eje, V. I., & Akuodor, G. C. (2023). Evaluation of the antiulcer and antimicrobial activities of methanol leaf extract of Helianthus annuus. International Journal of Basic & Clinical Pharmacology, 12(2), 161–166. https://doi.org/10.18203/2319-2003.ijbcp20230381

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Original Research Articles