A study to evaluate the in-vivo anticancer activity of ethanolic extract of Holoptelea integrifolia leaves against Ehrlich ascites carcinoma model using Swiss albino mice

Pramod S. G., Jayanthi M. K., Mruthunjaya K., Manjula S. N.


Background: Herbs having ethnomedical uses is one of the best approaches in searching novel anticancer drugs. The aim of the present study is to evaluate the anticancer activity of ethanolic extract of Holoptelea integrifolia leaves against Ehrlich ascites carcinoma induced liquid tumor model using Swiss Albino mice.

Methods: Acute toxicity test was performed using Wistar albino rats before starting the in-vivo anticancer activity, were the MTD was more than 5000 mg/kg. Animals were divided into six groups of six animals each. 250mg/kg and 500 mg/kg of ethanolic extract of HI leaves, was administered orally for 9 days and Cisplatin (3.5 mg/kg, i.p., single dose). Various parameters like Change in body weight, Mean Survival Time, Percentage Increase in Life Span, Hematological & Biochemical parameters were assessed.

Results: All the parameters were considerably restored towards the normal values. HIAL500mg/kg showed more significant results than 250 mg/kg. Hence 500mg/kg was taken for combination study with standard drug Cisplatin.

Conclusions: On the basis of the above result it was suggested that, the in-vivo anticancer activity of ethanolic extract of Holoptelea integrifolia leaves possess significant anticancer property with the dose dependent effect. This may probably due to the presence of phytochemicals such as alkaloids, phenols and flavonoids.


Holoptelea integrifolia, Cisplatin, Ethanolic extract, Ehrlich Ascites Carcinoma, Swiss albino mice

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