Comparative study of Eugenia jambolana seed and pulp for antidiabetic action on alloxan induced diabetic rats
Keywords:Antidiabetic, Eugenia jambolana, Alloxan, Ethanol extract
Background: Eugenia jambolana Lam., commonly known as black plum or “jamun” is an important medicinal plant in various traditional systems of medicine. It is effective in the treatment of diabetes mellitus, inflammation, ulcers and diarrhoea and preclinical studies have also shown it to possess chemopreventive, radioprotective and antineoplastic properties. The aim of the present study is to compare the action of extract of Eugenia jambolana (Jamun) seed with pulp on blood glucose level in alloxan-induced diabetic albino rats.
Methods: The extracts (100 mg/kg/d) were administered orally for four weeks to alloxan-induced diabetic rats. Blood glucose was estimated every week for four consecutive weeks.
Results: The test drugs that is Eugenia jambolana seed showed more significant (p<0.05) than pulp in reduction of blood glucose induced by alloxan.
Conclusions: Thus, Eugenia jambolana seeds show more antidiabetic effect than Eugenia jambolana pulp. However, further studies to isolate the active principle of Eugenia jambolana responsible for hypoglycemia, together with studies on serum insulin assay to confirm its insulin releasing action have to be undertaken.
Warrier P, Nambiar V, Ramankutty C. Indian Medical Plants. Volume 5. Hyderabad:Orient Longman Ltd;1996:225-228.
Ayyanar M, Subash-Babu P, Ignacimuthu S. Syzygium cumini (L.) Skeels., a novel therapeutic agent for diabetes: folk medicinal and pharmacological evidences. Complement Ther Med. 2013;21(3):232-43.
Power AC. Diabetes Mellitus. Harrison`s principles of internal medicine. Vol-2. 17th Edn. New York, NY: Mc Graw Hill Companies; 2008:2275.
King H, Aubert RE, Herman WH. Global burden of diabetics 1995-2025: prevalence numerical estimates and projections. Diabetes care. 1998;21:1413-4.
Bhatia IS, Bajaj KL. Chemical constituents of the seeds and bark of Syzygium cumini. Planta Medica. 1975;28:346-52.
Gordon A, Jungfer E. Phenolic constituents and antioxidant capacity of four underutilized fruits from the Amazon region. J Agricultural Food Chem. 2011;59:7688-99.
Sagrawat H, Mann A, Kharya M. Pharmacological potential of Eugenia jambolana: a review. Pharmacogenesis Magazice. 2006;2:96-104.
Rastogi R, Mehrotra B. Compendium of Indian medicinal plants. Central Drug Research Institute, Lucknow. 1990;1:388-9.
Pharmaceutical formulas. 11th ed. London: Chemist and Druggist Publishers; 1950;1:183-184.
Kumar A, Ilavarasan R, Jayachandran T, Decaraman M, Aravindhan P, Padmanabhan N, Krishan MRV. Phytochemical Investigation on a Tropical Plant, Syzygium cumini from Kattuppalayam, Erode District, Tamil Nadu, South India. Pakistan J Nutr. 2009;8(1):83-5.
Gowenlock AH, Janet R, Mc Murray D, Mc L. Glucose and other sugars and ketones. Valley’s practical clinical biochemistry. 6th ed. New Delhi; CBS publisher and distributors; 2002:321-323.
Chiplonkar SA, Rao KV. Analysis of variance. In: Rao KV, editor. Biostatistics: a manual of statistical methods for use in health, nutrition and anthropology. 1st Edn. New Delhi: Jaypee Brothers, Medical Publishers (P) Ltd.; 1996:237-272.
Rao KV, Balakrishna N. Multiple comparison test procedures: relative utility. In: Rao KV, editor. Biostatistics: a manual of statistical methods for use in health, nutrition and anthropology. 1st Edn. New Delhi: Jaypee Brothers, Medical Publishers (P) Ltd; 1996:273-284.
Kulkarni SK. Handbook of experimental pharmacology. #rd revised Edn. Delhi: Vallabh prakashan publishers; 2004:183-185.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50:536-46.
Kaneto H, Fujii J, Myint T, Miyazawa N, Islam KN, Kawasaki Y, et al. Reducing sugars trigger oxidative modification and apoptosis in pancreatic cells by provoking oxidative stress through the glycation reaction. Biochem J. 1996;320:855-63.
Chakkvarthy BK, Gupta S, Gambir SS, Gode KD. Pancreatic beta cell regeneration. A novel antidiabetic mechanism of Pterocarpus marsupium Roxb. Indian J Pharma. 1980;12:123-7.
Pietta P, Simonetti P Mauri P. Antioxidant activity of selected medicinal plants. J Agric Food Chem. 1998;46:4487-90.
Evans WC. Trease and Evans Pharmacognosy. 15th ed. Philadelphia: Saunders, an imprint of Elsevier Limited; 2002:214-252.
Kaneto H, Kajimoto Y, Miyagawa J, Matsuoka T, Fujitani Y, Umayahara Y, et al. Beneficial effects of antioxidants in diabetes: possible protection of cells against glucose toxicity. Diabetes. 1999;48:2398-406.