In-vitro anti-oxidant studies on ethanolic extract of Alpinia galanga linn
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20161868Keywords:
Antioxidant assays, Nitric oxide, Free radicals, Alpinia galanga, Vitamin EAbstract
Background: The free radical scavenging activity by in-vitro antioxidant assay is performed on ethanolic extract of Alpinia galanga. Free radicals are highly reactive molecules produced as a by-product during metabolism of oxygen. From the current understanding of pathophysiology, it is extensively proved about the positive role of reactive oxygen species in degenerative disease. Nature has provided abundant fruits, vegetables and medicinal plants with rich source of antioxidants as the natural defense against free radical induced damage to living organisms.
Methods: The present study is to screen and document the antioxidant property of Alpinia galanga from Zingiberaceae family by lipid peroxidation, nitric oxide and 1,1-diphenyl-2-picrylhydrazil radical scavenging in vitro antioxidant assay.
Results: The results are the percentage inhibitory concentration (IC) of Alpinia galanga are as follows IC50 102.70 mcg/ml, IC50 63.35 mcg/ml and IC50 8.80 mcg/ml and for control vitamin E is IC50 29 mcg/ml, IC50 15 mcg/ml and IC50 18 mcg/ml by Lipid peroxidation, 1,1-diphenyl-2-picrylhydrazil and nitric oxide free radical scavenging activity respectively.
Conclusions: The Alpinia galanga root ethanolic extracts from Zingiberaceae family has significant nitric oxide free radical scavenging activity.
References
Abdollahi M, Ranjbar A, Shadnia S, Nikfar S, Rezaiee A. Pesticides and oxidative stress: a review. Med Sci Monit. 2004;10:141-7.
Ames BN, Shigenega MK, Hagen TM. Oxidants and the degenerative diseases of ageing. Proc Nati Acad Sci. 1993;90:7915-22.
Shenoy R, Shirwaikar A. Anti-inflammatory and free radical scavenging studies of Hyptis suaveolens (labiatae). Indian drugs. 2002;39:574-7.
Patil S, Jolly CI, Narayanan S. Free radical scavenging activity of Acacia catechu and Rotula aquatica: implications in cancer therapy. Indian drugs. 2003;40:328-32.
Buyukokuroglu ME, Gulcin I, Oktay M, Kufrevioglu OI. In-vitro antioxidant properties of dantrolene sodium. Pharmacol Res. 2001;44:491-4.
Bergendi L, Benes L, Durackova Z, Ferencik M. Chemistry, physiology and pathology of free radicals. Life Sci. 1999;65:1865-74.
World Health Organisation. Traditional medicine. 2008. Available at http:// www.who.int/ mediacentre/factsheets /fs134/en/. Accessed on 23 April 2016.
Ohkawa H, Ohishi N, Yagi K. Assay of lipid peroxides in animal tissues by thio babituric acid reaction. Anal Biochem. 1979;95:346-51.
Sabina EP, Rasool M. Therapeutic efficacy of Indian ayurvedic herbal formulation triphala on lipid peroxidation, antioxidant status and inflammatory mediator TNF- α in adjuvant-induced arthritic mice. Int J Biol Chem. 2007;1:149-55.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate and in biological fluids. Anal Biochem. 1982;126:131.
Koleva II, Van Beek TA, Linssen JPH, De-Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis. 2002;13:8-17.
Matsuda H. Gastroprotectjve effects of phenylpropanoids from the rhizomes of Alpinia galanga in rats: structural requirements and mode of action. European J Pharmacol. 2003;471:59-67.
Subash KR, Muthulakshmi Bhaarathi G, Cheriyan BV. Phytochemical screening and acute toxicity study of ethanolic extract of Alpinia galanga in rodents. Int J Med res Health Sci. 2012;2(1):93-100.
Al-Nimer MSM, Ali EA. Dual effects of interaction between meloxicam, diclofenac sodium or tramadol and nitrogen species radicals: In vitro comparative study. Int J Pharmacol. 2009;5:86-9.
Subapriya R, Kumaraguruparan R, ramahandran CR, Nagini S. Antioxidant status in patients with oral squamous cell carcinoma at different oral sites. Clin Biochem. 2002;35:489-93.
Medeiros MV, Binhara IM, Morena JH, Zatz R, De Nucci, Antunes E. Effect of chronic nitric oxide synthesis inhibition on the inflammatory responses induced by carrageenan in rats. Eur J Pharmacol. 1995;285:109-11.
Sarkar D, Dutta A, Das M, Sarkar K, Mandal C, Chatterjee M. Effect of Aloe vera on nitric oxide production by macrophages during inflammation. Indian J Pharmacol. 2005;37:371-5.
Landino LM, Crews BC, Timmons MD, Morrow JD, Marnett LJ. Peroxynitrite the coupling product of nitric oxide and superoxide activates prostaglandin synthesis. Proc Natl Acad Sci USA. 1996;93:15069-74.
