Evaluation of analgesic and anti-inflammatory properties of the Allium cepa extract in rats
Keywords:Allium Cepa, Analgesic and anti-inflammatory activity, Rats
Background: The onion plant (Allium cepa) is the most widely cultivated species of the genus Allium. It has antibiotic, antidiabetic, antihypercholesterolemic, antioxidant, hemostatic, antimutagenic effects. The current study is aimed to evaluate analgesic and anti-inflammatory properties of Allium cepa extracts in rat models.
Methods: In this study analgesic activity in rats is tested by Eddy’s hot plate method, tail flick method. In Eddy’s hot plate method rats are individually placed on the heated plate of the analgesic meter maintained at 550C and mean reaction time was calculated at regular time intervals. In tail flick method the tail withdrawal from the heat (flicking response) is taken as the end point. Anti-inflammatory property is studied by carrageenan induced paw edema model, in which the paw volume is measured with a plethysmograph.
Results: In Eddy’s hot plate method the mean reaction time increased significantly (p<0.05) with high dose of Allium cepa at 1 hour and 2 hours when compared to control. In tail flick method the mean reaction time is increased significantly (p<0.05) with high dose of Allium cepa at 1 hour and 1.5 hour when compared to control. In carrageenan induced paw edema method Allium cepa exhibited anti-inflammatory activity by significant (p<0.05) suppression of paw edema when compare to control at 1 hour after carrageenan injection to rat paw.
Conclusions: Allium cepa has significant analgesic and anti-inflammatory activity in hot plate, tail flick and carrageenan induced paw edema model.
Devi B, Sharma N, Kumar D, Jeet K. Morusalbalinn: a phytopharmacological review. Int J Pharm Pharm Sci. 2013;5(2):14-8.
Michael W, Rabow SZ, Pantilat SS, Ramana KN. Palliative care & pain management In: Maxine AP, Stephen JM. Current medical diagnosis & treatment. 58th ed. USA: McGraw Hill; 2019:82.
Frederickson NF, David M. Introduction to immunity and inflammation. In: Bruntom LL, Lazo JS, Parker KL. Goodmans and Gilman’s the pharmacological basis of therapeutics. 13th ed. USA: McGraw Hill; 2018:630.
Brickell C. The royal horticultural society encyclopedia of gardening. USA: Dorling Kindersley; 1992:345.
Griffiths G, Trueman L, Crowther T, Thomas B, Smith B. Onions - A global benefit to health. Phytother Res. 2002;16(7): 603-15.
Sivam, GP. Protection against Helicobacter pylori and other bacterial infections by garlic. J Nutr. 2001;131(3):1106-8.
Akash MS, Rehman K, Chen S. Spice plant Allium cepa: dietary supplement for treatment of type 2 diabetes mellitus. Nutrition. 2014;30(10):1128-37.
Marrelli M, Amodeo V, Statti G, Conforti F. Biological Properties and Bioactive Components of Allium cepa L.: Focus on Potential Benefits in the Treatment of Obesity and Related Comorbidities. Molecules. 2018;24(1):119.
Boyle SP, Dobson VL, Duthie SJ, Kyle JA, Collins AR. Absorption and DNA protective effects of flavonoid glycosides from an onion meal. Eur J Nutr. 2000;39(5):213-23.
Kalus U, Pindur G, Jung F, Mayer B, Radtke H, Bachmann K, Mrowietz C, Koscielny J, Kiesewetter H. Influence of the onion as an essential ingredient of the Mediterranean diet on arterial blood pressure and blood fluidity. Arzneimittelforschung. 2000;50(9): 795-801.
Marefati N, Ghorani V, Shakeri F. A review of anti-inflammatory, antioxidant, and immunomodulatory effects of Allium cepa and its main constituents. Pharm Biol. 2021;59(1):287-302.
Gupta R, Tyagi P, Prakash J. Analgesic and anti-inflammatory drugs. In: Gupta SK eds. Drug screening methods. 2nd ed. New Delhi: Jaypee Brothers Medical; 2009:461-97.
Lanzottiv A. The analysis of onion and garlic. J Chrmotogr. 2006;11(12):213-20.
Lindegaard C, Gleerup KB, Thomsen MH, Martinussen T, Jacobsen N, Andersen PH. Anti-inflammatory effects of intra-articular administration of morphine in horses with experimentally induced synovitis. AJVR. 2010;71(1):69-75.
Dorsch W, Wagner H, Bayer T, Fessler B, Hein G, Ring J, et al. Anti-asthmatic effects of onions: Alkenyl sulfinothioic acid alkenyl-esters inhibit histamine release, leukotriene and thromboxane biosynthesis in vitro and counteract PAF and allergen-induced bronchial obstruction in vivo. Biochem Pharm.1988;37(23):4479-86.
Alpsoy S, Aktas C, Uygur R, Topcu B, Kanter M, Erboga M, et al. Antioxidant and anti-apoptotic effects of onion (Allium cepa) extract on doxorubicin-induced cardiotoxicity in rats. J Appl Toxicol. 2011;13(5):258-66.
Recio MC, Giner RM, Manes S, Gubells L, Gueh J, Julien HR, HoststtmannK. Anti-inflammatory activity of flavonol glycosides from Erythro-spermummonticolum depending on single or repeated local TPA administration. Planta Med. 1995;61(6):502-4.
Nouette-Gaulain K, Jose C, Capdevila X, Rossignol R. From analgesia to myopathy: When local anesthetics impair the mitochondrion. Int J Biochem Cell Biol. 2011;43(1):14-9.
Randolph BC, Peters MA. Analgesic effectiveness of ketorolac compared to meperidine in the rat formalin test. J Anesth Prog. 1997;44(1):11-6.
Willis WD. Role of neurotransmitters in sensitization of pain responses. Ann NY Acad Sci. 2001;933:142-56.
Yassaka RT, Hidetoshi H, Tsuchiyoshi FT. Enhanced activation of the transient receptor potential channel TRPA1 by ajoene, an allicin derivative. Neurosci Res. 2010;66(1):99-105.