Effect of ethanolic extract of Centella asiatica on pentylene tetrazol induced seizure in albino mice
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20204101Keywords:
Centella asiatica, Epilepsy, Pentylene tetrazolAbstract
Background: Various preparations of the plant Centella asiatica were used in traditional medicine for treating memory disorders, insanity, epilepsy, headache, fever and dysentery. Hence this study was conducted to test the effect of Centella asiatica on classical animal model of epilepsy.
Methods: First the ethanolic extract of Centella asiatica was subjected to acute toxicity study. Intraperitoneal injection of pentylene tetrazol in albino mice produces seizure. Plant extract was evaluated for protection from these experimental seizures. Four groups of animals were given the extract orally at 100mg, 200mg, 500mg and 1000mg per kilogram body weight. One group received phenobarbitone as standard drug and another group received distilled water as control. Time of onset of clonus is the study parameter. Statistical analysis of data was done using analysis of variance followed by Dunnet's test.
Results: Various doses of plant extract ranging from 100mg to 1000mg/kg exhibited protection from experimentally induced seizures. Compared to control, the plant extract showed statistically significant delay in time of onset of clonus.
Conclusions: Centella asiatica is potential source of anti-epileptic drug. Detailed phytochemical studies and animal experiments are recommended.
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References
Raymond DA, Maurice Victor, Allan HR. Principles of neurology. 6th ed. New York: Oxford University Press; 1997:335-8.
Rang HP, Dale MM, Ritter JM. Pharmaco. 4th ed. Edinburgh: Churchill Livingstone; 1999:575-77.
Chopra RN, Chopra IC, Handa KL, Kapur LD. Indigen Drug Ind. Calcutta: Academic Publishers. 1994:48-52.
Warrier PK, Nambiar VP, Ramankutty C. Indian Medicinal Plants, A Compendium of 500 Species. Madras: Orient Longm Ltd. 1997;2(1):52-5.
Bhishagratna KL. Sushrutasamhita English Translation. 1st ed. Vol. 2. New Delhi: Cosmo publications; 1996:523.
Sivaraman P, Shah HK, Vahora T. Investigation of Dimagheen used in unani medicine for learning and memory. Indi Dru. 2003;40:160-5.
Nadkarni KM. Indian Materia Medica. Bombay: Popular Prakashan. 1976;3(1):934-5.
Seshadri K, Srinivasan R. Antianxiety effect of an ayurvedic compound drug cross over trial. J Reasea Ayurve Siddh. 1992;13:107-16.
Mukherji B. Indian Pharmaceutical Codex. 1st ed. New Delhi: Council of Scientific and Industrial Research; 1953:60.
Ghosh GK. Herbs of Manipur. New Delhi: APH Publishing Corporation; 1992;1(2):280-1.
Rosenthaler L. The Chemical Investigations of Plants. 1st ed. London: G Bell and Sons Ltd; 1930:67-69.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy 13th ed. Pune: Nirali Prakashan Publisher. 2002;13:11-4.
OECD guideline for the testing of chemicals. Acute oral toxicity in animals. Adopted 17th December. 2001.
Ghosh MN. Fundamentals of Experimental Pharmacology. Calcutta: Hilton and Company. 1984:147.
Fischer RS, Animal models of epilepsies. Bra Resea Revi.1959;14:245-8.
Swinyard, Brown, Goodman. Comparative assay of antiepileptic drugs in mice and rats. J Pharmaco Experime Therapeut. 1952;106:319-30.
Katzung BG, Basic and Clinical Pharmacology. New York, NY: McGraw-Hill. 2001;8:395-6.
Marc AD. The epilepsies and convulsive disorders. In Isselbacher, Braunwald, Wilson, Martin, Fauci, Kasper editors. Harrison's Principles of Internal Medicine. New York, NY: McGraw-Hill. 1994;5:2223-24.
Craig CR, Stitzel RE. Modern Pharmacology with clinical applications. 5th Ed. Boston, Little Brown and Company. 1994;5:413.
Gupta YK, Jatinder M, George B, Kulkarni SK. Methods and considerations for experimental evaluation of antiepileptic drugs. Ind J Physiol Pharmacol. 1999;43:25-43.
Somchit MN, Zurain S, Israf S, Moin. Antinociceptive and anti-inflammatory effects of Centella asiatica. Ind J Pharmac. 1978;10:377-80.
Vaidya A. The status and scope of Indian medicinal plants acting on CNS. I J Pharmaco. 1997;29:340-43.
Njimi T, Ayafar. Screening of some African medicinal plants for antiulcer activity. Phytother Resea. 1997;11:45-7.
Bhuvaneswari, Silambuchelvi P. The activity of enzymic antioxidants in selected green leaves. Indian journal of nutrition and dietetics 1998;35:1-3.
Srivastava, Shukla, Darokar. Antibacterial activity of Centella asiatica. Fitotera. 1997;68:466-67.
Patil H, Nagavi DS, Ramesh S, Vijayakumar. A study on the immunostimulant activity of Centella asiatica in rats. Indi Drugs. 1998;35:711-52.
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