Antibiotic susceptibility pattern of organisms causing surgical site infection

Saranya K. Lakshmi; Hema Narasimhe Gowda; Anuradha K.

doi:10.18203/2319-2003.ijbcp20203627

Authors

Saranya K. Lakshmi Department of Pharmacology, Mysore Medical College and Research Institute, Mysore, Karnataka, India
Hema Narasimhe Gowda Department of Pharmacology, Mysore Medical College and Research Institute, Mysore, Karnataka, India
Anuradha K. Department of Microbiology, Mysore Medical College and Research Institute, Mysore, Karnataka, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20203627

Keywords:

Antibiogram, Antibiotic resistance, Iatrogenic infection, Wound swab

Abstract

Background: Approximately 30-50% of antibiotic use in hospitals is now for pre-surgical prophylaxis. Selection of antibiotics for prophylaxis should be based on its activity against expected bacteria at the desired surgical site. Appropriate antibiotic prophylaxis can reduce the risk of postoperative morbidity and mortality. Abuse of antimicrobials increases the cost of treatment and supports the emergence of resistant bacteria. This study was undertaken with the primary objective of to test the antibiotic susceptibility pattern of organisms causing SSIs.

Methods: A descriptive study was carried out in general surgical wards of KR hospital, Mysore for a period of 18 months (2015-2016). The relevant data was collected from the case sheets of patients who were diagnosed with SSI. Pus samples were collected, and culture-sensitivity was done. Collected data were analysed using appropriate statistical tests.

Results: A total of 263 study subjects including both males and females were enrolled in the study. Out of 263 pus samples 92% were culture positive. The most common organisms causing SSI were E. Coli-ESBL (n=73) and MRSA (n=44). Gram-negative organisms were highly resistant to β-lactam antibiotics and Gram-positive organisms were resistant to cotrimoxazole, erythromycin, clindamycin, cefoxitin and ciprofloxacin.

Conclusions: Good hygienic measures should be followed by the patients and health-workers. A revision of hospital antibiotic policy according to the local antibiotic susceptibility pattern is recommended.

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Author Biographies

Saranya K. Lakshmi, Department of Pharmacology, Mysore Medical College and Research Institute, Mysore, Karnataka, India

Mysore Medical College and Research Institute ,Mysore

Hema Narasimhe Gowda, Department of Pharmacology, Mysore Medical College and Research Institute, Mysore, Karnataka, India

Professor of Pharmacology,Mysore Medical College and Research Institute, Mysore.

Anuradha K., Department of Microbiology, Mysore Medical College and Research Institute, Mysore, Karnataka, India

Professor and Head,Department of Microbiology,Mysore Medical College and Research Institute, Mysore.

References

Mujagic E, Zwimpfer T, Marti WR, Zwahlen M, Hoffmann H, Kindler C, et al. Evaluating the optimal timing of surgical antimicrobial prophylaxis: study protocol for a randomized controlled trial. Trials. 2014;15(188):1-11.

Prajapati V, Bhatt JD. Evaluation of rational usage of antimicrobial agents in the medicine department at tertiary teaching care hospital, Gujarat. Int J Pharm Sci Res. 2014;5(3):865-73.

George SS, Varghese SR, Samuel CJ. Evaluation of antibiotic prescribing patterns among medical practitioners in North India. Int J App Basic Med Res. 2013;2(8):952-7.

Odonkor ST, Addo KK. Bacteria resistance to antibiotics: recent trends and challenges. Int J Biol Med Res. 2011;2(4):1204-10.

Salkind AR, Rao KC. Antibiotic prophylaxis to prevent surgical site infections. Am Fam Physician. 2011;83(5):585-90.

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Analysis. 2016;6(2):71-9.

Akhi MT, Ghotaslou R, Beheshtirouy S, Asgharzadeh M, Pirzadeh T, Asghari B, Alizadeh N, Ostadgavahi AT, Somesaraei VS, Memar MY. Antibiotic susceptibility pattern of aerobic and anaerobic bacteria isolated from surgical site infection of hospitalized patients. Jundishapur J Microbiol. 2015;8(7).

Hubab M. Antibiotic susceptibility profile of bacterial isolates from post-surgical wounds of patients in tertiary care hospitals of Peshawar, Pakistan. J Pak Med Assoc. 2019;69(3):448.

Sawdekar H, Sawdekar R, Wasnik VR. Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to antibiotic agents at super specialty hospital, Amravati city, India. Int J Res Med Sci. 2015;3(2):433-9.

Adegoke AA, Mvuyo T, Anthony O, Steve J. Studies on multiple antibiotic resistant bacterial isolated from surgical site infection. Scient Res Essays. 2010;5(24):3876-81.

Mengesha RE, Kasa BGS, Saravanan M, Berhe DF, Wasihun AG. Aerobic bacteria in post-surgical wound infections and pattern of their antimicrobial susceptibility in Ayder Teaching and Referral Hospital, Mekelle, Ethiopia. BMC Res Notes. 20147:575.

Reichman DE, Greenberg JA. Reducing surgical site infections: a review. Rev Obstet Gynecol. 2009;2(4):212-21.

Madhusudan E, Jakribettu RP, Boloor R, Rao SN. Study of pre-surgical antimicrobial prescribing pattern and correlation with microbiological data in a tertiary care hospital. EC Microbiol. 2015;2(4):365-73.

Amare B, Abdurrahman Z, Moges B, Ali J, Muluken L. Postoperative surgical site bacterial infections and drug susceptibility patterns at Gondar University Teaching Hospital, Northwest Ethiopia. J Bacteriol Parasitol. 2011;2:126.

Akinkunmi EO, Adesunkanmi AR, Lamikanra A. Pattern of pathogens from surgical wound infections in a Nigerian hospital and their antimicrobial susceptibility profiles. Afr Health Sci. 2014;14(4):802-9.

Shaikh S, Fatima J, Shakil S, Rizvi SMD, Kamal MA. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi J Biol Sci. 2015;22(1):90-101.

Kahsay A, Mihret A, Abebe T, Andualem T. Isolation and antimicrobial susceptibility pattern of Staphylococcus aureus in patients with surgical site infection at Debre Markos Referral Hospital, Amhara Region, Ethiopia. Arch Public Health. 2014;72(1):16.

Mama M, Abdissa A, Sewunet T. Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia. Ann Clin Microbiol Antimicrob. 2014;13(14):1-10.

Pal N, Guhathakurtha R. Surgical site infection in surgery ward at a tertiary care hospital: the infection rate and the bacteriological profile. IOSR J Pharm. 2012;2(5):1-5.

Green BN, Johnson CD, Egan JT, Rosenthal M, Griffith EA, Evans MW. Methicillin-resistant Staphylococcus aureus: an overview for manual therapists. J Chiropract Med. 2012;11(1):64-76.

Raza MS, Chander A, Ranabhat A. Antimicrobial susceptibility patterns of the bacterial isolates in post-operative wound infections in a tertiary care hospital, Kathmandu, Nepal. Open J Med Microbiol. 2013;3(3):159-63.

Bhatt CP, Baidya R, Karki P, Shah RK, Miya R, Mahashate P, et al. Multi drug resistance bacterial isolates of surgical site infection. Open J Med Microbiol. 2014;4:203-9.

Akhi MT, Ghotaslou R, Beheshtirouy S, Asgharzadeh M, Pirzadeh T, Asghari B, et al. Antibiotic susceptibility pattern of aerobic and anaerobic bacteria isolated from surgical site infection of hospitalized patients. Jundishapur J Microbiol. 2015;8(7):e20309.

Dessie W, Mulugeta G, Fentaw S, Mihret A, Hassen M, Abebe E. Pattern of bacterial pathogens and their susceptibility isolated from surgical site infections at selected referral hospitals, Addis Ababa, Ethiopia. Int J Microbiol. 2016;2016:1-8.

Asok PNS, Lal S, San S, Fousiya KS, Sreeja PA. Infectious wounds: assessing risk factors, severity and anti-microbial sensitivity patterns. World J Pharm Pharm Sci 2016;5(7):1298-316.

Patil SB, Paramne A, Harsh S. antibiotic susceptibility of wound isolates in plastic surgery patients at a tertiary care centre. Indian J Plast Surg. 2016;49(2):198-205.