Evaluation of antioxidant effect of Salacia oblonga against aluminum chloride induced visceral toxicity in albino rats


  • S. Nathiya Department of Pharmacology,Dr. ALMPGIBMS, Taramani Campus, Chennai - 600 113,Tamil Nadu,India
  • A. Nandhini Department of Pharmacology,Dr. ALMPGIBMS, Taramani Campus, Chennai - 600 113,Tamil Nadu,India


Oxidative stress, Antioxidant, Aluminum chloride, Salacia oblonga


Background: Aluminum is present in several manufactured foods and medicines and is also used in water purification. It is known that aluminum induces an oxidative stress characterized by an increase in lipid peroxidation and depletion of antioxidants. Therefore, the present experiment was undertaken to determine the effectiveness of hydroalcoholic extract of root bark of Salacia oblonga (SOHE) in modulating the aluminum chloride (AlCl3) induced oxidative stress in rats.

Methods: Animals were assigned into four groups: control; AlCl3 300 mg/kg b.w.; Salacia 67 mg/kg; AlCl3 (300 mg/kg b.w.) plus Salacia (67 mg/kg b.w.), respectively. Rats were orally administered their respective doses daily for 36 days. The effect of these treatments in influencing the aluminum induced biochemical changes on liver, kidney, lungs, and heart were studied.

Result: The results showed that S. oblonga produced significant (p<0.05) reduction of malondialdehyde, while the activities of superoxide dismutase, reduced glutathione, glutathione S-transferase and catalase were positively modulated.

Conclusion: Our data demonstrated that S. oblonga protects against aluminum-induced oxidative stress, which is an important finding that further reinforces the antioxidant properties of this natural product.


El-Gendy AM. Amelioration of aluminium - Intake oxidative stress by some antioxidants in male albino rats. Egypt J Hosp Med. 2011;45:536-46.

Neelu S, Sharma N, Johri S. Phytotherapeutic approach against aluminium induced biochemical changes in albino mice. J Pharm Res Opin. 2011;1:121-5.

Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological Profile for Aluminium. Atlanta, GA: US Department of Health and Human Services, Public Health Service; 1990.

Alfrey AC, LeGendre GR, Kaehny WD. The dialysis encephalopathy syndrome. Possible aluminum intoxication. New Engl J Med. 1976;294:184-8. [DOI via Crossref]

Al-Hashem FH. Camel's milk alleviates oxidative stress and lipid peroxidation induced by chronic aluminum chloride exposure in rat's testes. Am J Appl Sci. 2009;6(11):1868-75. [DOI via Crossref]

Nan Z, Ping L, LiNa H. Protection of aluminum chelating agent against cardiac toxicity in rats exposed to aluminum chloride. J Shandong Univ. (Health Sci). 2009;4, 8:125-7.

Mohammadirad A, Abdollahi M. A systematic review on oxidant/antioxidant imbalance in aluminium toxicity. Int J Pharmacol. 2011;7:12-21. [DOI via Crossref]

Moumen R, Ait-Oukhatar N, Breau F. Aluminium increases xanthine oxidase activity and disturbs antioxidant status in the rat. J Trace Elem Med Bio. 2001;15:89-93. [DOI via Crossref]

Vaidyaratnam PS. In: Warrier PK, Nambiar VPK, Ramankutty C, editors. Indian Medicinal Plants: A Compendium of 500 Species. Madras, India: Orient Longman; 1996: 47-8.

Singh NK, Biswas A, Rabbani SI, Devi K, Khanam S. Preventive effect of hydroalcoholic extract of Salacia oblonga root bark on mitomycin-C induced DNA damage using micronucleus test system in rats. Pharmacologyonline. 2009;21:127-33.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979:95;351-5. [DOI via Crossref]

Sinha KA. Colorimetric assay of catalase. Anal Biochem. 1972;47:389-94. [DOI via Crossref]

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47:469-74. [DOI via Crossref]

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82:48670-7. [DOI via Crossref]

Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974;249:7130-9.

Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951;193:265-75.

Missel JR, Schetinger MR, Gioda CR, Bohrer DN, Packolski L, Zanatta N, et al. Chelating effects of novel pyrimidines in a model of aluminum intoxication. J Inorg Biochem. 2005;99:1853-7. [DOI via Crossref]

Nicholas JCB, Dawes PT, Davies JD, Freemon AJ. Persisting aluminium-related bone disease after cadaveric renal transplantation. Nephrol Dial Transplant. 1999;14:202-4. [DOI via Crossref]

Gibanananda R, Hussain SA. Oxidants. Indian J Exp Biol. 2002;40:1213-32.

Patra RC, Swarup D, Senapati SK. Effect of cadmium on lipid peroxides and superoxide dismutase in hepatic, renal and testicular tissues of rats. Vet Hum Toxicol. 1998;41:65-7.

Al-Hashem FH. Camel's Milk protect against aluminium chloride induced toxicity in liver and kidney of white albino rats. Am J Biochem Biotechnol. 2009;5:98-108. [DOI via Crossref]

Katyal R, Desigan B, Sodhi CP, Ojha S. Oral aluminium administration and oxidative injury. Biol Trace Elem Res. 1997;57:125-30. [DOI via Crossref]

Abubakar MG, Taylor A, Ferns GA. The effects of aluminium and selenium supplementation on brain and liver antioxidant status in the rat. Afr J Biotechnol. 2003;3(1):88-93.

Halliwell B. Free radicals, antioxidants and human disease: curiosity, cause and cosequence? Lancet. 1994;344:721. [DOI via Crossref]

Abubakar MG, Taylor A, Ferns GA. Aluminium administration is associated with enhanced hepatic oxidative stress that may be offset by dietary vitamin E in rats. Int J Exp Pathol. 2003;84:49-54. [DOI via Crossref]

Kumaran A, Karunnakaran RJ. In vitro antioxidant activities of methanol extracts of Phyllanthus amarus species from India. LWT-Swiss Soc Food Sci Technol. 2007;40:344-52. [DOI via Crossref]




How to Cite

Nathiya, S., & Nandhini, A. (2017). Evaluation of antioxidant effect of Salacia oblonga against aluminum chloride induced visceral toxicity in albino rats. International Journal of Basic & Clinical Pharmacology, 3(2), 315–319. Retrieved from https://www.ijbcp.com/index.php/ijbcp/article/view/428



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