Lead acetate toxicity on glucose level and liver enzymes ameliorated by camel’s milk in wistar albino rat

Authors

  • Marwan M. Draid Department of Pharmacology, Toxicology and Forensic,Biochemistry and Animal Nutrition Medicine, University of Tripoli-13662, Tripoli, Libya
  • Mujahed O. Bushwereb Department of Physiology, Biochemistry and Animal Nutrition Medicine, University of Tripoli-13662, Tripoli, Libya
  • Shadia G. Ramadan Department of Pharmacology, Toxicology and Forensic,Biochemistry and Animal Nutrition Medicine, University of Tripoli-13662, Tripoli, Libya

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20161580

Keywords:

Lead acetate, Camel milk, Blood glucose, Liver enzymes

Abstract

Background: The present work was conducted to investigate the effects of lead acetate intoxication on glucose and liver functions in albino rats, and the possible effectiveness of using camel milk to protect against lead induced toxicity.

Methods: Eighteen male albino rats were divided into three groups of six, the first was a control group, the second received orally lead acetate in water as      (2 ml saline containing 5 mg/kg body weight of lead acetate) and the third received the same lead acetate dose and supplemented with 2 ml of camel milk, the experiment lasted for three weeks.

Results: The results indicated that exposure of animals to lead acetate caused a significant increase (p<0.05) in the activities of aspartate aminotransferase (AST) and decrease (p<0.05) in the alanine aminotransferase (ALT) compared with control group. Treatment with camel milk seemed to offer a marked improvement of the blood glucose parameter and the liver enzymes compared with lead acetate group. The parameters were reversed towards the normal values significantly.

Conclusions: The ability of camel milk to reduce lead toxicity may relate to its antioxidant actions or enhancing, the metal chelating action. In conclusion, Supplementation of daily diets with camel milk may be recommended to improve the body in case of lead contamination.

References

Heskel DL. A model for the adoption of metallurgy in the ancient middle east. Current Anthropology, 1983;24:362-6.

Dioka CE, Orisakwe OE, Adenyl FA, Meludu SC. Liver and renal function tests in Artisans occupationally exposed to lead in Mechanic village in Nnewi, Nigeria. Int J Environ Res Health. 2004;1:21-5.

Hepp NM, Mindak WR and Cheng J. Determination of total lead in lipstick: Development and single lab validation of a microwave-assisted digestion, inductively coupled plasma-mass spectrometric method. J Cosmet Sci. 2009;60:405-14.

Herman DS, Geraldine M, Venkatesh T. Case report: evaluation, diagnosis, and treatment of lead poisoning in a patient with occupational lead exposure: a case presentation. J Occup Med Toxicol. 2007;2:7-10.

Farmer AA, Farmer AM. Concentrations of cadmium, lead and zinc in livestock feed and organs around a metal production centre in eastern Kazakhstan. Sci total Environ. 2000;257:53-60.

Ziegler EE, Edwards BB, Jensen RL, Mahaffey KR, Fomon SJ. Absorption and retention of lead by infants. Pediatr Res. 1978;12:29-34.

Bogden JD, Gertner SB, Christakos S, Kemp FW, Yang Z, Katz SR, et al. Dietary calcium modifies concentrations of lead and other metals and renal calbindin in rats. J Nutr. 1992;122:1351-60.

Barltrop D, Meek F. Effect of particle size on lead absorption from the gut. Arch Environ Health. 1979;34:280-5.

Ballatori N, Clarkson TW. Biliary secretion of glutathione and glutathione-metal complex. Fundam. Appl. Toxicol. 1985;5:816-31.

Dalvi RR, Robbins TJ. Comparative studies on the effect of cadmium, cobalt, lead, and selenium on hepatic microsomal mono oxygenase enzymes and glutathione levels in mice. J Environ Pathol Toxicol. 1978;1:601-7.

McGowan C, Donaldson WE. Effect of lead toxicity on the organ concentration of glutathione and glutathione related free amino acids in the chick. Toxicol Lett. 1987;38:265-70

Yagil R. Camels and camel milk. Italy: FAO (Food and Agricultural Organization of the UN).

Redwan El-RM, Tabll A. Camel lactoferrin markedly inhibits hepatitis C virus genotype 4 infection of human peripheral blood leukocytes. J Immunoassay Immunochem. 2007;28:267-77.

Saltanat H, Li H, Xu Y, Wang J, Liu F, Geng XH. The influences of camel milk on the immune response of chronic hepatitis B patients. 2009:25:431-3.

Althnaian T, Albokhadaim I, El-Bahr SM. Biochemical and histopathological study in rats intoxicated with carbontetrachloride and treated with camel milk. Springer Plus. 2013;2:57.

Sharmanov TS, Zhangabylov AK, Zhaksylykova RD. Mechanism of the therapeutic action of whole mare’s and camel’s milk in chronic hepatitis. Vopr Pitan. 1982;1:17-23.

Beg OU, Von Bahr-Lind Strom H, Zaidi ZH, Jornvall H. Characterristic of camel milk protein rich in prolline identifies a new beta casein fragment. Regulatory Peptides. 1989;15:55-61.

Sharmanov TS, Kadyrova RK, Shlygina OE, Zhakslykova RD. Changes in the indicators of radioactive isotopes studies of the liver of patients with chronic hepatitis during treatment with whole camel’s milk and mare’s milk. Voprsy Pitaniya. 1978;1:9-17.

Agrawal RP, Sahani MS, Tuteja FC. Hypoglycemic Activity of Camel Milk in Chemically Pancreatectomized Rats-An Experimental Study. Int J Diabetes Dev Ctries. 2005;25:75-9.

Snedecor GW, Cochran GW. Statistical methods. 6th Edition. Ames: Iowa State University Press; 1973.

Goyer RA. Lead toxicity: from overt to subclinical to subtle health effects. Environ Health Perspect. 1990;86:177-81.

Bellinger DC. Lead, Pediatrics. 2004;113:1016-22.

Mudipall A. Lead hepatotoxicity and potential health effects. Indian J Med Res. 2007;126:518-27.

Lyn Patrick ND. Lead toxicity part II: The role of free radical damage and the use of antioxidant in the pathology and treatment of lead toxicity (Review) Altern. Med Rev. 2006;11:114-27.

Al-Humaid AI, Mousa HM, El-Mergawi RA, Abdel-Salam AM. Chemical composition and antioxidant activity of dates and dates-camel-milk mixtures as a protective meal against lipid peroxidation in rats. Am J Food Technol. 2010;5:22-30.

Nabil MI, Eweis ES, El-Beltagi HS, Yasmin E. Effect of lead acetate toxicity on experimental male albino rat . Asian Pacific J of Tropical Biomedicine. 2012;2:41-6.

Agrawal RP, Dogra R, Mohta N, Tiwari R, Singhal S, Sultania S. Beneficial effect of camel milk in diabetic nephropathy. Acta Biomed. 2009;80:131-4.

Agrawal RP, Jain S, Shah S, Chopra A, Agarwal V. Effect of camel milk on glycemic control and insulin requirement in patients with type 1 diabetes: 2-years randomized controlled trial. Eur J Clin Nutr. 2011;65:1048-52.

Mohamad RH, Zekry ZK, Al-Mehdar HA. Camel milk as an adjuvant therapy for the treatment of type 1 diabetes: verification of a traditional ethnomedical practice. J Med Food. 2009;12:461-5.

Zagòrski O, Maman A, Yafee A, Meisles A, van Creveld C, Yagil R. Insulin in milk: a comparative study. Int J Anim Sci. 1998;13:241-4.

Bujanda L, Hijona E, Larzabal M, Beraza M, Aldazabal P, García-Urkia N, et al. Resveratrol inhibits nonalcoholic fatty liver disease in rats. BMC Gastroenterology. 2008;8:40-8.

Haque MM. Effects of calcium carbonate, potassium iodide and zinc sulfate in lead induced toxicities in mice. MS Thesis, Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh; 2005.

Berney PJ, Cote LM, Buck WB. Low blood lead concentration associated with various biomarkers in household pets. Am J Vet Res. 1994;55:55-62.

Hamad EM, Abdel-Rahim EA, Romeih EA. Beneficial effect of camel milk on liver and kidneys function in diabetic sprague-dawley rats. Int J Dairy Sci. 2011;6:190-7.

Hassoun EA, Stohs SJ. Comparative studies on oxidative stress as a mechanism for the fetotoxic of TCDD. endrin and lindane in C57BL/6J and DBA/2J mice. Teratology. 1995;51:186.

Abdou HM, Newairy AA. Hepatic and reproductive toxicity of lead in female rats and attenuation by flaxseed lignans. JMRI. 2006;27:295-302.

Singh B, Dhawan D, Garg ML, Mangal PC, Chand B, Trehan PN. Impact of lead pollution on the status of other trace metals in blood and alterations in hepatic functions. Biol Trace Elemen Res. 1994;40:21-9.

Allouche L, Hamadouche M, Touabti A, Khennouf S. Effect of long-term exposure to low or moderate lead concentrations on growth, lipid profile and liver function in albino rats. Advan Biol Res. 2011;5:339-47.

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Published

2016-12-30

How to Cite

Draid, M. M., Bushwereb, M. O., & Ramadan, S. G. (2016). Lead acetate toxicity on glucose level and liver enzymes ameliorated by camel’s milk in wistar albino rat. International Journal of Basic & Clinical Pharmacology, 5(3), 1125–1130. https://doi.org/10.18203/2319-2003.ijbcp20161580

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Original Research Articles