Evaluation of detoxifying activity of ORGCHP against acetaminophen induced hepatotoxicity in Sprague Dawley rats
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20202181Keywords:
Hepatotoxicity, Acetaminophen, Detoxification, Antioxidant, HepatoprotectiveAbstract
Background: Accumulation of toxins in the body over a period of time interferes with the normal body functioning. The removal of toxins from the body is called ‘detoxification’. The liver is the main organ involved in detoxification and damage to the liver may impede the removal of toxins. The present study assessed the hepatoprotective activity of an organic Chlorella vulgaris in acetaminophen-induced liver damage model.
Methods: A total of 35 animals were randomized to five groups with seven animals in each group. The test drug, organic Chlorella vulgaris (ORGCHP; 350 mg/kg and 700 mg/kg) was compared with a reference standard (200 mg/kg). The positive control group and the vehicle control group were administered 0.5% w/v carboxy methyl cellulose. All groups received dose volume of 10 ml/kg for 10 days. Acetaminophen was given on day 8 and day 9. Blood collections were done at baseline day 1, day 8 and day 10. Outcome measures were the change in body weight, oxidation biomarkers, histopathological evaluation, gross pathological evaluation and relative body weight.
Results: Test drug ORGCHP Chlorella vulgaris showed dose dependent reduction in hepatoxicity with high reduction in aspartate transaminase (AST) and modest lowering of alanine transaminase (ALT) (350 mg/kg; p.o.); while at high dose (700 mg/kg; p.o.) there was significant reduction in alkaline phosphatase (p<0.05), ALT (p<0.001) and AST (p<0.001) levels. No pathological or histopathological abnormality was seen in control group. In drug group, one animal showed minimal necrosis, while mild and moderate necrosis was seen in three animals each respectively.
Conclusions: The test drug exhibits detoxifying and hepatoprotective activity against acetaminophen induced hepatotoxicity.
References
Ernst E. Alternative detox. British Medical Bulletin. 2012;101(1):33-8.
Department of Health and Human Services Centers for Disease Control and Prevention. Fourth National Report on Human Exposure to Environmental Chemicals. 2009. Available at: https://www.cdc.gov/ exposurereport/pdf/fourthreport.pdf. Accessed on 12 December 2013.
MacIntosh A, Ball K. The effects of a short program of detoxification in disease-free individuals. Altern Ther Health Med. 2000;6:70-6.
Genues SJ, Sears ME, Schwalfenberg G, Hope J. Clinical Detoxification: Elimination of persistent toxicants from the Human body. Sci World J. 2013: 1-2.
Grant DM. Detoxification pathways in the liver. J Inherit Metab Dis. 1991;14(4):421-30.
Cullen JM, Stalker MJ. Liver and Biliary System. Jubb, Kennedy and Palmer's Pathology of Domestic Animals: Volume 2 (Sixth Edition). Elsevier; 2016.
Bjornsson ES. Hepatotoxicity by Drugs: The Most Common Implicated Agents. Int J Mol Sci. 2016;17(2):224.
Slaughter RL, Edwards DJ. Recent advances: the cytochrome P450 enzymes. Ann Pharmacother. 1995;29:619-24.
Gu X, Manautou JE. Molecular mechanisms underlying chemical liver injury. Expert Rev Mol Med. 2012;14.
Peng HY, Chu YC, Chen SJ, Chou ST. Hepatoprotection of chlorella against carbon tetrachloride-induced oxidative damage in rats. In Vivo. 2009;3(5):747-54.
Ahmad F, Tabassum N. Experimental models used for the study of antihepatotoxic agents. J Acute Dis. 2012;29:85-9.
David S, Hamilton JP. Drug-induced Liver Injury. US Gastroenterol Hepatol Rev. 2010;6:73-80.
Grunhage F, Fischer HP, Sauerbruch T, Reichel C. Drug and toxin-induced hepatotoxicity. Z Gastroenterol. 2003;41(6):565-78.
Cai X, Yang Q, Wang S. Antioxidant and hepatoprotective effects of pigment-protein complex from 2 Chlorella vulgaris on carbon tetrachloride-induced liver damage in vivo. RSC Advances. 2015;5:96097-104.
Serie AMM, Habashy NH, Attia WE. In vitro evaluation of the synergistic antioxidant and anti-inflammatory activities of the combined extracts from Malaysian Ganoderma lucidum and Egyptian Chlorella vulgaris. BMC Complement Altern Med. 2018;18(1):154.
Huyut Z, Beydemir S, Gulcin I. Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochem Res Int. 2017;2017:7616791.
Prabakarana G, Moovendhana M, Arumugam A, Matharasia A, Dineshkumara R, Sampathkumar P. Quantitative Analysis of Phytochemical Profile in Marine Microalgae Chlorella Vulgaris. Int J Pharmacy Biological Sci. 2018;8(2):562-5.
Huyut Z, Beydemir S, Gulcin I. Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochemistry Res Int. 2017;1:1-10.
Giannini EG, Testa R, Savarino V. Liver Enzyme Alteration: A Guide for Clinicians. Canadian Medical Association J. 2005;172(3):367-79.
