DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20161573

Effect of portulaca olearecea and its interaction with vecuronium on neuromuscular junction in experimental animals

Anjali Tarai, P. Ansuman Abhisek, S. N. Pandey

Abstract


Background: The extracts from the plant Portulaca oleracrea have been mentioned to have muscle relaxant properties in traditional medicine literatures and used as a food supplement. The site of therapeutic action is directly on muscle fibres rather than neuromuscular transmission.The muscle relaxant property is found to be similar with dantrolene in frog rectus abdominis .So objectives of our study to analyze the effect of Portulaca oleracea and its interaction with vecuronium on the neuromuscular junction in experimental animal.

Methods: The study was conducted in two parts i.e. study 1 and study 2. In study 1:2 ml and 4 ml Portulaca oleracea fresh aqueous leave juice was given orally to 12 number rabbits and vecuronium 5 microgram/ml was given intravenously to all 6 rats through marginal ear vein with the help of scalp vein attached with 10 ml syringe. The head drop time is recorded and reversal of neuromuscular blockade was done with neostigmine (0.25 mg/kg) along with atropine (2 mg). Study 2: 2 ml and 4 ml of Portulaca oleracea fresh aqueous leave juice was tested on frog's isolated rectus abdominis muscle in Dales’s isolated organ bath and its interaction with vecuronium (1 mmole-32 mmole) was recorded.

Results: Portulaca oleracea has muscle relaxant properties, which was recorded which was indicated by decreasing the time of head drop and complete block in study 1. In study 2, it also decreased the Ach induced contraction in presence of vecuronium.

Conclusions: The results indicate the muscle relaxant properties fresh juice of Portulac Oleracea probably due to high content of potassium.


Keywords


Portulaca oleracea, Vecuronium, Neuromuscular junction, Muscle relaxation

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References


Patel TK, Patel PB, Tripathi CB. Effect of pantoprazole and its interactions with vecuronium on the neuromuscular junction. IJP. 2010;42(1):36-9.

Englehar T, Webster NR. Pulmonary aspiration of gastric contents in anaesthesia. Br J Anaesthesia. 1999;83:453-60.

Albert CC, Baker JO, COOK JE. A rational approch to preanaesthetic medication drugs. 1989;37:219-28.

Okamoto TAC, Fukushimak T. Neuromuscular blocking effect of ORG9428 A new non depolarising muscle relaxtant and its recovery with various antagonist in vitro muscle.1992;41:1467-73.

Olssson GL, Hallen B, HambralchJonzon K. Aspiration during anaesthesia. A computer aided study of 185-358 anaesthetics. Altaanaesthesiolscaned. 1986;30:84-92.

Habtemariam S, Harvey AL, Waterman PG. The muscle relaxtant properties of portulacaoleracea are associated with high concentration of potassium ions J Ethnopharmacol. 1993;40(3):195-200.

Varney RE, Linegar LR, Hoalday HA. The assay of curare by rabbit head drop method. J Pharmacol Exp Ther. 1949;97:72-83.

Lix Howe P. Zhou YF, XU ZH, HocartC, Zhang R. Fatty acids and b-carotene in Australian purslane portulacaoleracea varieties. J Chomatogi. 2000;893:207-13.

Barbosa Filho JM, Alencar AA, Nunes XP, Tomaz AC, SenaFilho JG, Athhhayde Fiho PF, et al. Sources of alpha, beta, gamma, delta and epsilon-carotenes. Atweentieth century review Rev Bras Famacogn. 2008;18:135-54.

Chatterjee A, Chandra S, Pakrashi. The treatise on Indian medicinal plants. Pubimform Directorate. 1956;1243-4.

Smopoulous AP, Noman HA, Gillaspy, Duke JA. Common purslane. A source of omega -3 fatty acids and anti-oxidants. J Am Coll Nur. 1992;11:374-82.

Party O, Marks JA, Okwuasab FK. The skeletal muscle relaxant action of portulacaoleracea. Role of potassium ions. J Ethinopharmacol. 1993;49:187-94.