Evaluation of analgesic activity of Aegle marmelos steam bark in experimental animals


  • Sarang Gajanan Ghodki Department of Pharmacology, RKDF Medical College, Hospital and Research Centre, Bhopal, Madhya Pradesh, India
  • Sanjio Bhimrao Borade Department of Pharmacology, SMBT Medical College, Hospital and Research Centre, Nashik, Maharashtra, India
  • Harshal Pise Department of Pharmacology, Swami Ramanand Tirth Rural Govt. Medical College, Ambajogai, Maharashtra, India
  • Vijay Motghare Department of Pharmacology, Govt. Medical College, Nagpur, Maharashtra, India
  • Rekha Sanjay Mehani Department of Pharmacology, RKDF Medical College, Hospital and Research Centre, Bhopal, Madhya Pradesh, India
  • Prashant Wadgbalkar Department of Pharmacology, RKDF Medical College, Hospital and Research Centre, Bhopal, Madhya Pradesh, India




Aegle marmelos, AMSBAE, Analgesia, Tail flick method, Writhes


Background: Alleviation of pain has always remained a prime concern of medicine. Numbers of anti-inflammatory and analgesic drugs like aspirin, indomethacin, phenylbutazone etc. are in practice, but because of their side effects, there is extensive search for new drugs and molecules with fewer side effects. Search for newer anti-inflammatory and analgesic agents having better or at least equal efficacy with minimal side effects is continuing throughout the world. Therefore, the search should continue and it is felt that herbal medicine has still a lot in store. Aegle marmelos is one of the most frequently used plant in the traditional and folklore systems of medicine and in religious rituals. Various activities of different parts like roots, leaves, flowers, fruits and seeds of AM were evaluated. It has analgesic, anti-inflammatory, antipyretic, antidiabetic, antioxidant, hepatoprotective and many other activities.

Methods: Analgesic activities of AMSBAE (50,100 and 200 mg/kg p.o.) were studied using tail flick test and acetic acid-induced writhes in rats and mice respectively.

Results: AMSBAE produces dose dependent analgesic activity in the tail flick test and acetic acid-induced writhing in mice (P<0.05).

Conclusions: AMSBAE has analgesic activity. The analgesic activity of AMSBAE was comparable to that of tramadol and Aspirin. Hence, AMSBAE could be a possible alternative to NSAIDs.


IASP Sub-committee on Taxonomy. Pain terms: A list with definitions and notes on usage. Pain. 1980;8:249-52.

Patkar Atul N, Desai Nilesh V, Ranage Akkatai A, Kalekar Kamalakar S. A review on Aegle marmelos: a potencial medicinal tree. International Research Journal of Pharmacy. 2012;3(8):86-91.

Malviya R, Kumar A, Singh A, Kulkarni GT. Pharmacological screening, ayurvedic values and commercial utility of aegle marmelos. International Journal of Drug Development and Research. 2012;4(1):28-37.

Chopra RN, Dr. K. M. Nadkarni’s Indian Materia Medica. Popular Prakashan Pvt. Ltd. Mumbai; 1986:1:45-49.

Kirtikar KR, Basu BD. Indian Medicinal Plants. Periodical expert Book Agency, Delhi. 2nd ed.; 1984:1:499-502.

Brijesh S, Daswani P, Tetali P, Noshir A, Tannaz B. Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: Validating its traditional usage. BioMed Central Complementary and Alternative Medicine. 2009;9:47.

OECD Guidelines for the testing of chemicals. 425. Acute oral toxicity up-and-down-procedure (UDP); 2008. Available at http://iccvam.niehs.nih.gov/SuppDocs/FedDocs/OECD/OECDtg425.pdf.

D’Amour FE, Smith DN. A method for determining loss of pain sensation. Journal of Pharmacology and Experimental Therapeutics. 1941;72:74-9.

Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Federation Proceedings. 1959;18:412.

Wong CH, Day P, Yarmush JG, Wu WH, Zbuzek VK. Nifedipine induced analgesia after epidural injections in rats. Anesth Analg. 1994;79:303-6.

Knighton RS, Dumke PR. The production of analgesic activity in man by animal testing. In: Knighton RS, Dumke PR, editors. Pain. Boston: Little Brown and Co.; 1966:163-82.

Shankarananth V, Balakrishnan N, Suresh D, Sureshpandian G, Edwin E, Sheeja E. Analgesic activity of methanol extract of Aegle marmelos leaves. Fitoterapia. 2007;78(3):258-9.

Kothari S, Kushwah A, Kothari D. Involvement of opioid and monoaminergic pain pathways in Aegle marmelos induced analgesia in mice. Indian Journal of Pharmacology. 2013;49(4):371-5.

Jones CK, Petre SC, Shanon HE. Efficacy of duoloxetine a potent and balanced serotonergic and noradrenergic reuptake inhibitor in inflammatory and acute pain models. J Pharmacol Exp Ther. 2005;2:726-32.

Vogel HG. Analgesic, anti-inflammatory and antipyretic activity. In drug discovery and evaluation: Pharmacological assays. 2nd ed. Germany: Springer Verlag Berlin Heidelberg. 2002;2:716-7.

Liju Vijayastelter B, Kottarapat J, Kuttan R. An evaluation of antioxidant, anti-inflammatory, and antinociceptive activities of essential oil from Curcuma longa. L Indian Journal of Pharmacology. 2011;43(5):526-31.

Gupta M, Kanti MU, Kumar RS, Kumar TS. Studies on anti-inflammatory, analgesic and antipyretic properties of methanol extract of caesalpinia bonducella leaves in experimental animal models. Iranian Journal of Pharmacology and Therapeutics. 2003;2(2):30-4.

Arul V, Miyazaiki S, Dahananjaya R. Studies on the anti-inflammatory, antipyretic, analgesic properties of the leaves of Aegle marmelos. Journal of Ethnopharmacology. 2005;96(1-2):159-63.

Bhavani Shankar R, Nagaraja Perumal G, Tirumal Rao K, Sravanthi D, Omer MA, Phukon M. A preliminary study on analgesic and hypoglycemic activity of 50% ethanolic extract of Aegle marmelos (corr.) leaves and its phytochemical studies. Contemporary Investigations and Observations in Pharmacy. 2012;1(2):92-5.




How to Cite

Ghodki, S. G., Borade, S. B., Pise, H., Motghare, V., Mehani, R. S., & Wadgbalkar, P. (2016). Evaluation of analgesic activity of Aegle marmelos steam bark in experimental animals. International Journal of Basic & Clinical Pharmacology, 5(3), 1081–1086. https://doi.org/10.18203/2319-2003.ijbcp20161572



Original Research Articles