Published: 2020-01-24

Evaluation of cytotoxic profile of hydroalcoholic extract of fruit rinds of Garcinia pedunculata on human embryonic kidney and breast carcinoma cells

Sukrant Sharma, Ravi Mundugaru, Pradeepa H. Dakappa, Pundalik R. Naik


Background: The fruit rinds of Garcinia pedunculata has potential medicinal properties and used in many chronic ailments. It has been demonstrated that cytoprotective effects in various experimental research works. But its cytotoxic effect has not been evaluated. The present study was aimed to screen its relative cytotoxic effect on normal and cancer cell lines.

Methods: In the present study, the cytotoxic effect of hydro alcoholic extract of Garcinia pedunculata was evaluated on normal human embryonic kidney (HEK-293) and M.D. Anderson metastatic breast cancer cell lines (MDA-MB 231) using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay.

Results: Higher dose level of hydro alcoholic extract of Garcinia pedunculata (HAGP) (500 μg/ml) has shown considerable increase (112.503) in the percentage viability of HEK-29 whereas; there is a remarkable decrease in the viable cell population (77.490) in MDA-MB 231.

Conclusions: Based on the observed results we could conclude that HAGP has potential cytotoxic effect on the cancer cell line without altering the normal cell growth and proliferation. Thus it has potential to develop as a safer chemotherapeutic agent. Further detailed exploration is required to confirm its therapeutic efficacy in different cancer cell lines.


Human embryonic kidney cells, Metastatic breast cancer, Cisplatin, Nephroprotective, Cytotoxic

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Kagyung R, Gajurel PR, Rethy P. Singh B. Ethnomedicinal plants used for gastrointestinal diseases by Aditribes of Dehang-Debang Biosphere Reserve in Arunachal Pradesh. Indian J Tradit Know. 2010;9:496-501.

Sahu A, Das B, Chatterjee A. Polyisoprenylated benzophenones from Garcinia pedunculata. Phytochemistry. 1989;28(4):1233-5.

Jayaprakasha GK, Jena BS, Sakariah KK. Improved liquid chromatographic method for determination of organic acids in leaves, pulp, fruits, and rinds of Garcinia. J AOAC Int. 2003;86(5):1063-8.

Ravi M, Senthilkumar S, Padmaja U, Sudhakara B. Cardio protective activity of fruits extract of Garcinia pedunculata. Bangladesh J Pharmacol. 2016;11:5-9.

Ravi M, Madhan C, Ravishankar B. Hepatoprotective activity of fruits extract of Garcinia pedunculata. Bangladesh J Pharmacol. 2014;9:4-8.

Negi PS, Jayaprakasha GK, Jena BS. Antibacterial activity of the extracts from the fruit rinds of Garcinia cowa and Garcinia pedunculataagainst food borne pathogens and spoilage bacteria. LWT-Food Sci Technol. 2008;41(10):1857-61.

Jayaprakasha GK, Negi PS, Jena BS. Antioxidative and antimutagenic activities of the extracts from the rinds of Garcinia pedunculata. Innovative Food Science Emerging Technologies. 2006;7(3):246-50.

Mundugaru R, Joy F, Shrinidhi R, Das L, Sudhakara RB. Anti-inflammatory activity of aqueous extract of fruits of Garcinia pedunculatain experimental animals. Am J Pharma Tech Res. 2014;4(3).

Ravi M, Sivanesan S, Febin J, Sunil Kumar KN, Lekshmi R, Alfarhan AH, et al. Quality Standardization and Nephroprotective Effect of Garcinia Pedunculata roxb. Fruit rind. IJPER. 2017:51:136-44.

Mosmann T. Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays. J Immunol Methods.1983;65(1-2):55-63.

Desai AG, Qazi GN, Ganju RK, El-Tamer M, Singh J, Saxena AK, et al. Medicinal plants and cancer chemoprevention. Curr Drug Metab. 2008;9(7):581-91.