DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20200170

A study to evaluate efficacy of Tinospora cordifolia (Guduchi) as analgesic agent using albino wistar rats as an experimental animal model

Purnendu Mandal, Asoke Kumar Das, Ananya Mandal, Tuhin Kanti Biswas

Abstract


Background: Pain is a very well-known symptom of many diseases and analgesics are used to relieve pain. The main problem with these drugs remains that of side effects. Herbal medicines are better in view of their cultural acceptability, better compatibility with human body systems and lesser incidence of side effects. Extract of Tinospora cordifolia (Guduchi) plant have been traditionally used to treat pain in traditional medicine.

Methods: Commercially available preparation of T. cordifolia plant has been used as test drug (aqueous extract). Healthy albino rats of either sex, weighing between 140-200 g were selected for the study, divided into 4 groups of 6 each (control, standard, 100 mg/kg, 300 mg/kg). Central analgesic activity was assessed by tail flick model (morphine as standard drug I.P). Acetic acid 1% 10 ml/kg aqueous solution I.P. was used for abdominal writhing model. Diclofenac 150 mg/kg oral as standard drug for assessment of peripheral analgesic activity. Results were analysed using SPSS version 16 and Microsoft office excel 2007.

Results: T. cordifolia extract significantly increased the tail flick latency time (sec) (mean tail flick latency control, T100, T300 6.833±0.25 sec, 8.65±017 sec, 10.01±0.14 respectively) (p value control vs T100, T300 at 90 min, 120 min, 0.0573, 0.0198, 0.0198 in between group) and decreased number of abdominal writhing in comparison with the control group (p value <0.0001).

Conclusions: Extract of T. cordifolia was found to possess analgesic activity and also exhibited dose and time dependant increase involving central and peripheral mechanisms. The analgesic activity of T. cordifolia found to be comparable to standard drug used.


Keywords


Analgesic, T. cordifolia, Abdominal writhing, Tail flick

Full Text:

PDF

References


Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014;4:177.

Aggarwal B, Prasad S, Reuter S. Identification of Novel Anti-Inflammatory Agents from Ayurvedic Medicine for Prevention of Chronic Diseases: ‘Reverse Pharmacology’ and ‘Bedside to Bench’ Approach. Curr Drug Targets. 2011;12:1595-653.

Calixto JB. Twenty-five years of research on medicinal plants in Latin America: a personal view. J Ethnopharmacol. 2005;100:131-4.

Ganapati M. Mandatory rural practice proposed in India. BMJ. 1995;311:1186.

Loeser JD. The Kyoto protocol of IASP Basic Pain Terminology. Pain. 2008;137:473-77.

Dewan SMR, Amin MN, Adnan T. Investigation of analgesic potential and in vitro antioxidant activity of two plants of Asteraceae family growing in Bangladesh. J Pharm Res. 2016;6:599-603.

Ministry of Environment, Forest and Climate Change Government of India. Compendium of CPCSEA. New Delhi: CPCSEA; 2018: 60-87.

Indian Council of Medical Research, New Delhi. 2008. Available at: https://www.icmr.nic.in/sites/ default/files/guidelines/GCLP.pdf. Accessed on 5 December 2019.

Ministry of Environment, Forest and Climate Change Government of India. Compendium of CPCSEA. New Delhi: CPCSEA; 2018: 62.

Karnam N. Screening of a novel-substituted furan compound for analgesic activity in mice. Int J Basic Clin Pharmacol. 2017;5:1759-63.

Paget GE. Evaluation of Drug Activities and Pharmacometrics: London NY: Academic Press; 1964.

UBC Animal Care Guidelines, SOP: ACC-2012-Tech09. Available at: ACC-2012-Tech09 Oral Dosing (Gavage) in the Mouse and Rat) Updated Feb 2015 final_cc, ka.pdf. Accessed on 5th Dec 2019.

UBC Animal Care Guidelines. SOP: ACC‐2012‐Tech10. Available at: https://animalcare.ubc.ca/sites/ default/files/documents/TECH%2010%20IP%20Injections%20in%20the%20Mouse%20and%20Rat.pdf. Accessed on 5th Dec 2019.

Kulkarni S. Handbook of Experimental Pharmacology. 4th edition. New Delhi: Vallabh Publication; 2012.

Medhi B, Prakash A. Practical Manual of Experimental and Clinical Pharmacology: Jaypee Brothers Medical Publishers (P) Ltd; 2010: 225-27.

OECD guideline for testing of chemicals. Available at: https://ntp.niehs.nih.gov/iccvam/suppdocs/ feddocs/oecd/oecd_gl423.pdf. Accessed on 5th Dec 2019.

Goel B, Pathak N, Nim DK, Singh SK, Dixit RK, Chaurasia R. Clinical Evaluation of Analgesic Activity of Guduchi (Tinospora Cordifolia) Using Animal Model. J Clin Diagn Res. 2014;8:1-4.

Hussain L, Akash MSH, NUA, Rehman K, Ibrahim M. The Analgesic, Anti-Inflammatory and Anti-Pyretic Activities of Tinospora cordifolia. Adv Clin Exp Med. 2015; 24:957-64.

Siddalingappa CM, RajeshT, Kudagi BL, Krishnakanth K, Sujith TR. Evaluation of analgesic and anti- inflammatory activities of Tinospora cordifolia in rodents. Int J Basic Med Sci. 2011;2:447.