Antinociceptive effect of methanolic extract of Murraya koenigii leaves in swiss albino mice

Arunkumar J., Vijayalakshmi M., Yesodha S., YousufAli A. S., Parthiban R.


Background: The objective of the study was to evaluate anti-nociceptive effect of methanolic extract of Murraya koenigii leaves on thermal and mechanical pain in swiss albino mice.

Methods: Thirty adult male swiss albino mice weighing 25-30 grams were selected and allocated in to five groups. Each group consists of six animals. The control group received vehicle (10 ml/kg), standard group received morphine (10 mg/kg) and test groups received dried methanolic extract of Murraya koenigii leaves (100 mg/kg, 200 mg/kg, 400 mg/kg per oral respectively) 1 hour before placing the animal over the hot plate at temperature of 55⁰C . A cut off period of 10 sec was observed to avoid damage of the paw. The response in the form of withdrawal of paws or licking of the paws. The delay in the reaction time denotes analgesic activity. The latency was recorded before and after 15, 30, 60, 120 minutes administration of drug. After washout period of 1 month the same group of animals were utilized to evaluate the analgesic effect by tail clip method for better comparison.

Results: All the doses of Murraya koenigii leaves significantly delayed reaction time in hot plate method and tail clip method. The results were comparable to that produced by standard drug morphine.

Conclusions: Murraya koenigii leaves has analgesic activity which was comparable to morphine.


Analgesia, Hot plate method, Murraya koenigii, Tail clip method

Full Text:



Rang HP, Dale MM, Ritter JM. Pharmacology. 5th ed. New York: Churchill Livingstone; 2003: 217-227.

Michel YB, Dubois, Christopher G, Allen HL. Chronic pain management. In: Healy TEJ, Knight PR, eds. Wylie and Churchil-Davidson’s A practice of Anaesthesia. 7th edition. London: Hodder Arnold; 2003: 1235-1139.

Satyavati GV, Raina MK, Sharma M. Medicinal plants of India. Indian Council Med Res. 1987;2:289-99.

Kirtikar KR, Basu BD. Indian medicinal plants. Indian Medicinal Plants. 2nd edition. 1993: 472-474.

Rao LJ, Ramalakshmi K, Borse B, Raghavan B. Antioxidant and radicalscavengingcarbazole alkaloids from the oleoresin of curry leaf (MurrayakoenigiiSpreng.) Food Chem. 2007;100:742-7.

Chakrabarty M, Nath AC, Khasnobis S, Chakrabarty M, Konda Y, Harigaya Y, et al. Carbazole alkaloids from Murraya koenigii. Phytochem. 1997;46:751-5.

Vogel HG, Vogel WH, Scholkens BA, Sandow J, Muller G, Vogel WF. Drug discovery and evaluation 2nd edition. Berlin Heidelberg: Springer-Verlag; 2002: 716-717.

Ghosh MN. Fundamentals of experimental pharmacology. 5 th ed. Kolkata: Hilton and company; 2008:151-152.

Bianchi C, Franceschini J. Experimental observations on Haffner's method for testing analgesic drugs. Brit J Pharmacol Chemother. 1954;9(3):280-4.

Haffner F. Experimentelle prüfung schmerzstillender mittel. DMW-Deutsche Medizinische Wochenschrift. 1929;55(18):731-3.

Sharmin Ani N, Chakraborty S, Moniruzzaman M. The Methanolic Extract from Murraya koenigii L. Inhibits Glutamate-Induced Pain and Involves ATP-Sensitive K. Adv Pharmacol Sci. 2016: 1-6.

Gupta S, George M, Singhal M, Sharma GN, Garg V. Leaves extract of Murraya koenigii Linn for anti-inflammatory and analgesic activity in animal models. J Adv Pharmaceut Technol Res. 2010;1(1):68-77.

Patil RA, Langade PM, Dighade PB, Hiray YA. Antinociceptive activity of acute and chronic administration of Murraya koenigii. leaves in experimental animal models. Indian J Pharmacol. 2012;44:15-9.

Kartik SJ, Mirunalini R, Jervin M, Manimekalai K. Evaluation of analgesic activity of Murraya koenigii and Coriandrum sativum leaves extract in animal model. Asian J Pharmaceut Clini Res. 2018;11(1):328-31.