Analgesic and anti-inflammatory activity of hydroalcoholic extract of Piper betle leaves in experimental animals


  • Pooja S. Reddy Department of Pharmacology,Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha district, Maharashtra- 442102, India
  • Rajesh K. Gupta Department of Pharmacology,Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha district, Maharashtra- 442102, India
  • Srikanth M. Reddy Department of Psychiatry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha district, Maharashtra- 442102, India



Piper betle leaf, Sub-therapeutic potential, Analgesic, Anti-inflammatory


Background: Piper betle leaf, commonly known as ‘paan’ has long been known for its various medicinal properties in traditional medicine but certain properties have remained less explored. We tried to assess the analgesic and anti-inflammatory activities of Piper betle leaves.

Methods: Hydroalcoholic extract of Piper betle leaves (HEPBL) was extracted using soxhlet apparatus and its phytochemical analysis was performed. Wistar rats and Albino mice were used for all the experiments. Acute toxicity study was also done according to OECD guideline no.425 and the test doses were decided accordingly. The experimental models of tail-flick method and acetic acid induced writhing were used to study the analgesic activity whereas carrageenan induced paw edema and cotton pellet granuloma models were used for anti-inflammatory action. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Dunnett's test.

Results: HEPBL showed significant analgesic activity at the doses of 100 mg/kg and 200 mg/kg, and showed significant anti-inflammatory activity at the doses of 50 mg/kg, 100 mg/kg and 200 mg/kg. The sub-therapeutic dose of HEPBL at 50 mg/kg also potentiated the analgesic effect of sub-therapeutic doses of standard analgesics. The analgesic and anti-inflammatory activity of P.betle may be attributed to the presence of various phyto constituents’ viz. flavonoids, tannins, phenols and glycosides.

Conclusions: HEPBL has significant analgesic and anti-inflammatory activity in experimental animals in our study.


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How to Cite

Reddy, P. S., Gupta, R. K., & Reddy, S. M. (2016). Analgesic and anti-inflammatory activity of hydroalcoholic extract of Piper betle leaves in experimental animals. International Journal of Basic & Clinical Pharmacology, 5(3), 979–985.



Original Research Articles