Influence of Tinospora cordifolia on wound healing in wistar rats


  • Nayana K. Hashilkar Department of Pharmacology, KLE University’s J. N. Medical College, Belagavi, Karnataka, India
  • Paragouda A. Patil Department of Pharmacology, USM-KLE international Medical Programme, Belagavi, Karnataka, India
  • Jayasheela G. Bagi Department of Physiology,KLE University’s J. N. Medical College, Belagavi, Karnataka, India
  • Sunita Y. Patil Department of Pathology KLE University’s J. N. Medical College, Belagavi, Karnataka, India
  • Netravathi B. Angadi Department of Pharmacology, KLE University’s J. N. Medical College, Belagavi, Karnataka, India



Excision wound, Dead space wound, Resutured incision wound, Tinospora Cordifolia


Background: T.cordifolia is widely used in veterinary folk medicine ayurvedic system of medicine. It is known to possess various properties. In a study T.cordifolia promoted wound healing in diabetic patients. However, one study showed that octacosanol content in T.cordifolia possessed anti-angiogenic activity which can hinder wound healing. Therefore, effect of T.cordifolia on wound healing appears to be controversial and there is scarcity of information regarding its effect on wound healing in animal models.

Methods: Excision wound, resutured incision wound and dead space wounds were inflicted under light thiopentone anesthesia in male wistar rats (n=6 in each group). Methanol extract of T.cordifolia stem in the dose of 250 mg/kg was administered orally once a day for 10 days in resutured incision (assessed by wound breaking strength), dead space (granuloma dry weight and histopathology of granulation tissue) excision wounds was monitored by planimetry. Data was expressed as mean±SEM and analyzed by student’s t-test. p <0.05 was considered as significant.

Results: The results of the present study revealed that T.cordifolia significantly promotes wound healing in all the three models viz. enhanced wound contraction and decreased days for complete epithelization in excision wound; increased breaking strength in resutured incision wound; increased granuloma dry weight and cellular infiltration in granulation tissue.

Conclusions: T.cordifolia significantly (p<0.05) promoted wound healing in all the three models of wound in male wistar rats.


Kumar V, Abbas AK, Fausto N, Mitchell RN. Robbins- Basic Pathology. 8th Edition Published by Elsevier at Thomson Press India Ltd; 2007:59-79.

Kissane JM. Anderson’s Pathology 9th ed. St. Louis: The C.V. Mosby Co; 1990:67-109

Atalay M, Oksala N, Lappalainen J, Laaksonen DE, Sen CK, Roy S. Heat shock proteins in diabetes and wound healing. Current Protein and Peptide Science. 2009;10(1):85-95.

Din SU, Volk S, Bayat A. Regenerative healing, scar-free healing and scar formation across the species: current concepts and future perspectives. Experimental Dermatology. 2014;23:615-9.

Adams SB, Sabesan VJ, Easley ME. Wound healing agents. Foot Ankle Clin. 2006;11:745-51.

Jorge BA, Schultz GS, Ernesto LM, Gerardo GN, Marianela GS, Luis HM. Glucose toxic effects on granulation tissue productive cells: the diabetics’ impaired healing. BioMed Research International. 2013;ID 256043:15.

Goforth KNF, Harken AH, Saba JD. Normalization of diabetic wound healing. Published in final edited form as. Surgery. 2010;147(3):446-9.

Liu R, Bal HS, Desta T, Behl Y, Graves DT. Tumor necrosis factor mediates diabetes enhanced apoptosis of matrix-producing cells and impairs diabetic healing. American Journal of Pathology. 2006;168(3):757-64.

Steed DL. The role of growth factors in wound healing. Surg Clin North Am. 1997;77:575-86.

Singla S, Singla S, Arun Kumar, Singla M. Role of epidermal growth factor in healing of diabetic foot ulcers. Indian J Surg. 2012;74(6):451-5.

Allen JR, Soares MA, Haberman ID, Schachar CSJ, Lin CD, Nguyen PD, et al. Combination therapy accelerates diabetic wound closure. PLOS ONE. | 2014;9(3):e92667.

Wieman TJ, Smiell JM, SU Y. Efficacy and safety of a topical formulation of recombinant human platelet derived growth factor-BB (becaplermin) in patients with chronic neuropathic diabetic ulcers. A phase III randomised placebo controlled double- blind study. Diabetes Care. 1998;21:822-7.

Purandare H, Supe A. Immunomodulatory role of Tinospora cordifolia as an adjuvant in surgical treatment of diabetic foot ulcers: a prospective randomized controlled study. Indian Journal of Medical Sciences. 2007;61(6):347-55.

Singh SS, Pandey SC, Shrivastava S, GuptaVS, Patro B, Ghosh AC. Chemistry and medicinal properties of Tinospora cordifolia (Guduchi). Ind J Pharmacol. 2003;35:83-91.

Mishra A, Kumar S, Pandey A. Scientific validation of the medicinal efficacy of Tinospora cordifolia. The Scientific World Journal. 2013;ID 292934:8.

Chopra RN, Chopra LC, Handa KD, Kapur LO. Editors indigenious drugs of India, 2nd ed, Kolkata: M/S Dhar VN and sons; 1992.

Upadhyay AK, Kumar K, Kumar A, Mishra HS. Tinospora cordifolia (Willd.) Hook F and Thoms. (Guduchi)-Validation of the Ayurvedic pharmacology through experimental and clinical studies. Int J Ayurveda Res. 2010;1(2):112-21.

Rajalaxmi M, Eliza J, Priya CE, Niramala A, Daisy P. Anti-diabetic properties of Tinospora cordifolia stem extracts on streptozocin-induced diabetic rats. African Journal of Pharmacy and Pharmacology. 2009;3(5):171-80.

Thippeswamy G, Sheela ML, Salimath BP. Octacosanol isolated from Tinospora cordifolia downregulates VGEF gene expression by inhibiting nuclear translocation of NF-B and its DNA binding activity. Eur J Pharmacol. 2008;588(2-3):141-50.

Jagetia GC, Rao SK. Evaluation of antineoplastic activity of Guduchi (Tinospora cordifolia) in Ehrlich ascites carcinoma bearing mice. Biol Pharm Bull. 2006;29:460-6.

Patil PA, Kulkarni DR. Effect of antiproliferative agents on healing of dead space wounds in rats. Indian J Med Res. 1984;79:445-7.

Sivakumar V, Dhana Rajan MS. Standardization and characterization of Tinospora cordifolia (Willd.) Meirs ex Hook F and Thoms. Plant stem extract in different solvent fractions. Asian Journal of Biochemical and Pharmaceutical Research. 2011;4(1):105-12.

Morton JJP, Malone MH. Evaluation of vulnary activity by an open wound procedure in rats. Arch Int Pharmacodyn. 1972;196:117-26.

Ehrlich HP, Hunt TK. Effects of cortisone aid on the tensile strength of healing wounds. Ann Surg. 1969;170:633-41.

Lee KH. Studies on the mechanism of action of salisylates: retardation of wound by aspirin. J Pharm Sci. 1968;57:1042-3.

D’Arcy PF, Howard EM, Muggleton PW, Jownsend SB. The anti-inflammatory action of Griseofulvin in experimental animals. J Pharm Pharmacol. 1960;17:379-82.

Dipasquale G, Meli A. Effect of body weight changes on the formation of cotton pellet induced granuloma. J Pharm Pharmacol. 1965;17:379-82.

Ambrish C, Torgal SS, Patil PA, Malur PR, Hiremath SV. Influence of oral anti-diabetic agents on wound healing in euglycemic male Wistar rats. Pharmacologyonline. 2009;1:476-83.

Jagetia GC, Rao SK. Evaluation of cytotoxic effects of Dichloromethane extract of Guduchi (Tinospora cordifolia Miers ex Hook F and THOMS) on cultured HeLa cells. Evid Based Complement Alternat Med. 2006;3(2):267-72.

Saha S, Ghosh S. Tinospora cordifolia: One plant, many roles. Anc Sci Life. 2012;31(4):151-9.

Cantürk NZ, Vural B, Esen N, Cantürk Z, Oktay G, Kirkali G, et al. Effects of granulocyte-macrophage colony-stimulating factor on incisional wound healing in an experimental diabetic rat model. Endocr Res. 1999;25(1):105-16.

Fang Y, Shen J, Yao M, Beagley KW, Hambly BD, Bao S. Granulocyte-macrophage colony-stimulating factor enhances wound healing in diabetes via upregulation of proinflammatory cytokines. Br J Dermatol. 2010;162(3):478-86.




How to Cite

Hashilkar, N. K., Patil, P. A., Bagi, J. G., Patil, S. Y., & Angadi, N. B. (2016). Influence of Tinospora cordifolia on wound healing in wistar rats. International Journal of Basic & Clinical Pharmacology, 5(3), 923–928.



Original Research Articles