Evaluation of analgesic activity of aqueous extract of leaves of hibiscus sabdariffa in albino rats
Keywords:H. sabdariffa Linn, Analgesic, Indomethacin, Albino rats
Background: Analgesia and inflammation continues to be an area of great interest for research, probably due to the non-availability of a safer and more effective analgesic and anti-inflammatory agent. This has led to increase in demand for natural products with analgesic and anti-inflammatory activity having fewer side effects. Non-steroidal anti-inflammatory drugs (NSAIDs) such as indomethacin are used in the treatment of inflammation, fever and pain. However, NSAIDs cause gastric damage as a major adverse reaction. In this study, the analgesic and anti-inflammatory activity of aqueous extract H. sabdariffa Linn was studied using hot plate method in albino rats.
Methods: In this study, analgesic activity of H. sabdariffa was studied and compared with standard drug, indomethacin using Hot plate method. Aqueous extract of leaves of H. sabdariffa (200 mg/kg or 400 mg/kg) and indomethacin (25 mg/kg) were used to separate groups of rats and reaction time was measured at 20, 60, 90 minutes and compared with control group.
Results: All the test groups showed significant (P<0.05) analgesic effect in increasing the reaction time in hot plate method during the entire test period of 90 minutes.
Conclusions: H. sabdariffa possesses significant analgesic activity probably due to presence of flavonoids and organic acids like ascorbic acid and citric acid as well as polysaccharides reported to be present in this plant.
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